DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kusmierczyk-2011-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218560X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-2 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218573X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-3 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218569X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-4 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218565X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-5 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218561X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-6 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218566X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-7 ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218570X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-8 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218562X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-9 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218571X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-10 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218563X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-11 ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218567X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-12 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218568X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-13 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218572X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation kusmierczyk-2011-14 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. DIP original experiment: DIP-218564X Nature structural & molecular biology 2011 IMEx A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR., Kunjappu MJ., Kim RY., Hochstrasser M. Only protein-protein interactions imex curation ump1_yeast Proteasome maturation factor UMP1 UMP1 YBR1234 YBR173C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNIVPQDTFKSQVSTDQDKSVLSSAVPSLPDTLRQQEGGAVPLSTQLNDRHPLESTLKNWETTQRQRQMEQYRQIFGIAEPMKRTMEMEIVNRTDFNPLSTNGSIHRDILLNKECSIDWEDVYPGTGLQASTMVGDDVHSKIEKQLGI 7C774DD40F3FCD7C poc1_yeast Proteasome chaperone 1 YLR199C PBA1 POC1 Proteasome biogenesis-associated protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLFKQWNDLPEPKHLLDLPEISKNLQSLEVCPVPKVEFPQDLDVPQYSTAVITTKIMNPLFPKNLLQLTSIGEIKTTLTVKSPSLPQSSGKHSWNYDENFPNEVDPDQKNDTADETVYGFSFPIYSFGKTLLFSMEENFISISPIFGNMISRSIISQLAQFSPDIIVIGTSDKIASMKVMTENECTLQPPEFITGFIGSVLTQLIVGPSKGLKFKCLVAPSEGPNGFEKLSLSDMGSLVDLCGQWLGFEPSRYSEECYRLWRCDSAAIGAQSGLYI 783C930795771748 q6lys4_metmp MMP0914 protein protein metmp Methanococcus maripaludis (strain S2 / LL) MEYVSKKEIKYKEPLVISGFPGIGLVGSIASYHLLKNLKMEYVGYIEDPMIPEIMIVEEGIAYPPVRVYARDDLVIFFSDVMIPPELVYPMSVMISDRLKEINPKMVVTLEGFASMTPEKSFWVSSSEKILNSVKDEEIPALQLGMIGGISGALMKCCNDRDIPAACLITETVGLRPDPRGASKIIENLNKKYNLNADTEELIKEAENIEEKMKSLAKEHAKLMSKPKTENPMYM psb_metmp Proteasome subunit beta psmB 20S proteasome beta subunit Proteasome core protein PsmB MMP0695 protein protein metmp Methanococcus maripaludis (strain S2 / LL) MISNSEYHKEYMKGTTTVGLLCNDGVVLATDKRATMGNLIADKEAKKLYKIDDYIAMTIAGSVGDAQSLIRLISAEAKIYKMRTGNNMTPLSCTTLISNVLHGNRHYPLLTQLILGGYDLINGAKLFSLDPVGGINEESSFTATGSGSPTAYGVLESEYKSDIAIEKGLLIAVKALSSAMQRDAYSGNGISLAHINKDGVKLYSDAEIEGFLKKINRKR psa_metmp Proteasome subunit alpha psmA 20S proteasome alpha subunit Proteasome core protein PsmA MMP0251 protein protein metmp Methanococcus maripaludis (strain S2 / LL) MQQMVPASGYDRAITIFSPEGRLYQVEYAREAVRRGTTAVGIKCKDGVVLAVDRRITSKLIDVSSIEKIFQIDDHIVAATSGLVADARVLIDRARIEAQMNRVSYGEAITVEALAKKICDIKQAYTQHGGARPFGLALLITGIDRHSARLFETDPSGALIEYKATAIGSGRPIAMEVLESKYDENMTVSEGMELALYALSKTTEELKPENIDMAIIKDSGKLVEKISVDEIEKIVKAVYEKVKAEEEEAEKNKGEEDSE q6lwu3_metmp MMP1611 protein protein metmp Methanococcus maripaludis (strain S2 / LL) MVKIIENKLIDAEPLKDAVLIEGLPGIGHVGRVAAEHIVEEFNGEKVLELYCDDFPPQIVVKNDGTIEYMKNEFYLVKEPVPMVIVLGNTQALSPKGQYVLSEKIVDIAMKYGATKTYTLGGFGIGKISDELKVYVASTSKELSEQHKELGAEFRTDGGSIIGAAGLMLKFSKLKGIEGICLMGETPGYLVDPKSAGNVLEMLSKAIGFEIDMKKLDERAKEMEKFIEKVQMQEQGMQAPQHQDDLSYIG poc2_yeast Proteasome assembly chaperone 2 YKL206C ADD66 PBA2 POC2 Proteasome biogenesis-associated protein 2 Alpha-1-proteinase inhibitor-degradation deficient protein 66 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSCLVLPLVSVGNIPQLSIDWLLNSQANEWEYLEALDSKYLVEFVGPLDRPEDGSDSLYKDADMKYSSALEVFYNKKRGLFAIQQRTPLVSVNYLNNFIVEIILPFLSKYNISEICIWDSLYAMEDENGVIVRPQEVYSLGEFYFDDEAELLSNLHLNDQESMVNNWLHFTPTSFQDKISVDQPIFKILFQILNASQRPKALRSIKYCSCLANEGDNSLDSQQFLQWIISQKVIKNAPPIVKFVRPISWQGAYGMADARDKFVDLYN D635C3EBC2E37C11 pba1-ump1-1 3225146 N/A 3225160 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3225161 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false psma-psmb-2 3225147 N/A 3225162 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3225163 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3225164 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false psmb-psma-1 3225148 N/A 3225163 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3225164 unspecified role unspecified role prey prey Region 14-259 Region 14-259 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false q6lwu3_metmp-q6lys4_metmp-1 3225149 N/A 3225165 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3225162 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association false false false q6lys4_metmp-q6lwu3_metmp-1 3225150 N/A 3225165 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3225162 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false psma-psmb-3 3225151 N/A 3225163 unspecified role unspecified role prey prey Mature protein Region 15-219 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3225164 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false psma-psmb-4 3225152 N/A 3225163 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3225164 unspecified role unspecified role unspecified role unspecified role Region 14-259 Region 14-259 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false q6lys4_metmp-q6lwu3_metmp-2 3225153 N/A 3225165 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3225162 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false psmb-psma-2 3225154 N/A 3225162 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3225163 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3225164 unspecified role unspecified role prey prey ecolx Escherichia coli association association false false false psma-q6lwu3_metmp-1 3225155 N/A 3225164 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3225165 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association false false false psma-psmb-5 3225156 N/A 3225163 unspecified role unspecified role unspecified role unspecified role Mature protein Region 15-219 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3225164 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pba2-pba1-1 3225157 N/A 3225166 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3225161 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association false false false psma-psmb-6 3225158 N/A 3225162 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3225163 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3225164 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli association association false false false psma-q6lys4_metmp-1 3225159 N/A 3225162 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3225164 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false