DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ jore-2011-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218541X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation jore-2011-2 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218538X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation jore-2011-3 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218544X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation jore-2011-4 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218545X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation jore-2011-5 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218542X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation jore-2011-6 in vitro In vitro ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218548X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation jore-2011-7 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218539X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation jore-2011-8 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218546X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation jore-2011-9 in vitro In vitro ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218549X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation jore-2011-10 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218540X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation jore-2011-11 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218543X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation jore-2011-12 ecolx Escherichia coli ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218547X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation jore-2011-13 ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. DIP original experiment: DIP-218550X Nature structural & molecular biology 2011 IMEx Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER., Waghmare SP., Wiedenheft B., Pul U., Wurm R., Wagner R., Beijer MR., Barendregt A., Zhou K., Snijders AP., Dickman MJ., Doudna JA., Boekema EJ., Heck AJ., van der Oost J., Brouns SJ. Only protein-protein interactions imex curation cas6_ecoli CRISPR system Cascade subunit CasE casE CasE endoRNase cas6e crRNA endonuclease ygcH JW2726 b2756 protein protein ecoli Escherichia coli (strain K12) MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKRNTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKRLDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQTVCFEGVLTINDAPALIDLVQQGIGPAKSMGCGLLSLAPL cas5_ecoli CRISPR system Cascade subunit CasD casD cas5 ygcI b2757 JW5844 protein protein ecoli Escherichia coli (strain K12) MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACLGIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHETIQTWREYLCDASFTVALWLTPHATMVISELEKAVLKPRYTPYLGRRSCPLTHPLFLGTCQASDPQKALLNYEPVGGDIYSEESVTGHHLKFTARDEPMITLPRQFASREWYVIKGGMDVSQ casc_ecoli CRISPR system Cascade subunit CasC casC cas4 cse4 ygcJ JW2728 b2758 protein protein ecoli Escherichia coli (strain K12) MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSLKRAMRKSGYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKSVDEAEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDIALSGRMATSGMMTELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQEFSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPADMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSLSDVDPITAQVKQMPTLEQLKSWVRNNGEA cse2_ecoli CRISPR system Cascade subunit CasB casB cse2 ygcK JW2729 b2759 protein protein ecoli Escherichia coli (strain K12) MADEIDAMALYRAWQQLDNGSCAQIRRVSEPDELRDIPAFYRLVQPFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKSEQTTGISLGRALANSGRINERRIFQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA cse1_ecoli CRISPR system Cascade subunit CasA casA cse1 ygcL JW2730 b2760 CRISPR type I-E/Ecoli-associated protein CasA/Cse1 CRISPR-associated protein CasA/Cse1 protein protein ecoli Escherichia coli (strain K12) MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDMELAALALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQTKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGGGFKSGLRGGTPVTTFVRGIDLRSTVLLNVLTLPRLQKQFPNESHTENQPTWIKPIKSNESIPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGLWPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQFRNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTALRKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANVNFSQADEVIADLRDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG casa-casb-1 3224523 N/A 3224536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224537 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224538 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224539 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224540 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false casb-casa-1 3224524 N/A 3224536 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3224537 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3224538 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3224539 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3224540 unspecified role unspecified role prey prey ecolx Escherichia coli association association false false false casa-casb-2 3224525 N/A 3224536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224537 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224538 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224539 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224540 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false casb-casc-1 3224526 N/A 3224536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224537 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224538 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224539 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false casb-casc-2 3224527 N/A 3224536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224537 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224538 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224539 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false casb-casc-3 3224528 N/A 3224536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224537 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224538 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224539 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false casb-casc-4 3224529 N/A 3224536 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3224537 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3224538 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3224539 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli association association false false false casc-casd-1 3224530 N/A 3224536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224537 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224538 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false casb-1 3224531 N/A 3224539 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false casc-casd-2 3224532 N/A 3224536 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3224537 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3224538 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli association association false false false casc-casd-3 3224533 N/A 3224536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224537 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224538 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false casa-casb-3 3224534 N/A 3224536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224537 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224538 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224539 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224540 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false casa-casb-4 3224535 N/A 3224536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224537 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224538 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224539 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3224540 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false