DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ vojnic-2011-1 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Vojnic E., Mourão A., Seizl M., Simon B., Wenzeck L., Larivière L., Baumli S., Baumgart K., Meisterernst M., Sattler M., Cramer P. DIP original experiment: DIP-218516X Nature structural & molecular biology 2011 IMEx Structure and VP16 binding of the Mediator Med25 activator interaction domain. Vojnic E., Mourão A., Seizl M., Simon B., Wenzeck L., Larivière L., Baumli S., Baumgart K., Meisterernst M., Sattler M., Cramer P. Only protein-protein interactions imex curation vojnic-2011-2 in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined Vojnic E., Mourão A., Seizl M., Simon B., Wenzeck L., Larivière L., Baumli S., Baumgart K., Meisterernst M., Sattler M., Cramer P. DIP original experiment: DIP-218517X Nature structural & molecular biology 2011 IMEx Structure and VP16 binding of the Mediator Med25 activator interaction domain. Vojnic E., Mourão A., Seizl M., Simon B., Wenzeck L., Larivière L., Baumli S., Baumgart K., Meisterernst M., Sattler M., Cramer P. Only protein-protein interactions imex curation g8hbg2_hhv11 Alpha trans-inducing protein UL48 Alpha-TIF protein protein hhv11 Human herpesvirus 1 (strain 17) (HHV-1) Human herpes simplex virus 1 HHV-1 MDLLVDELFADMNADGASPPPPRPAGGPKNTPAAPPLYATGRLSQAQLMPSPPMPVPPAALFNRLLDDLGFSAGPALCTMLDTWNEDLFSALPTNADLYRECKFLSTLPSDVVEWGDAYVPERTQIDIRAHGDVAFPTLPATRDGLGLYYEALSRFFHAELRAREESYRTVLANFCSALYRYLRASVRQLHRQAHMRGRDRDLGEMLRATIADRYYRETARLARVLFLHLYLFLTREILWAAYAEQMMRPDLFDCLCCDLESWRQLAGLFQPFMFVNGALTVRGVPIEARRLRELNHIREHLNLPLVRSAATEEPGAPLTTPPTLHGNQARASGYFMVLIRAKLDSYSSFTTSPSEAVMREHAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG 8DDDEDEDB2A699D3 med25_human Mediator of RNA polymerase II transcription subunit 25 MED25 ACID1 ARC92 PTOV2 Mediator complex subunit 25 Activator-recruited cofactor 92 kDa component Activator interaction domain-containing protein 1 p78 TCBAP0758 protein protein human Homo sapiens Human MVPGSEGPARAGSVVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQPPTDVSQDPRHMVLVRGLVLPVGGGSAPGPLQSKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPGNLSAAQVAAQNAVEAAKNQKAGLGPRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPGAPKPPPASQPSLVSTVAPGSGLAPTAQPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQQRGMGGQQAPPGLGPILEDQARPSQNLLQLRPPQPQPQGTVGASGATGQPQPQGTAQPPPGAPQGPPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPPAQSWPAQLPPRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI med25-vp16-3 3223202 N/A 3223204 unspecified role unspecified role unspecified role unspecified role Transactivation domain Region 452-490 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3223205 unspecified role unspecified role unspecified role unspecified role Activator interaction domain Region 394-543 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false med25-vp16-4 3223203 N/A 3223204 unspecified role unspecified role unspecified role unspecified role Transactivation domain Region 413-490 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3223205 unspecified role unspecified role unspecified role unspecified role Activator interaction domain Region 394-543 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q71SY5:p.Arg466Glu R466E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false