DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ milbradt-2011-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Milbradt AG., Kulkarni M., Yi T., Takeuchi K., Sun ZY., Luna RE., Selenko P., Näär AM., Wagner G. DIP original experiment: DIP-218513X Nature structural & molecular biology 2011 IMEx Structure of the VP16 transactivator target in the Mediator. Milbradt AG., Kulkarni M., Yi T., Takeuchi K., Sun ZY., Luna RE., Selenko P., Näär AM., Wagner G. Only protein-protein interactions imex curation milbradt-2011-2 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Milbradt AG., Kulkarni M., Yi T., Takeuchi K., Sun ZY., Luna RE., Selenko P., Näär AM., Wagner G. DIP original experiment: DIP-218515X Nature structural & molecular biology 2011 IMEx Structure of the VP16 transactivator target in the Mediator. Milbradt AG., Kulkarni M., Yi T., Takeuchi K., Sun ZY., Luna RE., Selenko P., Näär AM., Wagner G. Only protein-protein interactions imex curation milbradt-2011-3 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Milbradt AG., Kulkarni M., Yi T., Takeuchi K., Sun ZY., Luna RE., Selenko P., Näär AM., Wagner G. DIP original experiment: DIP-218514X Nature structural & molecular biology 2011 IMEx Structure of the VP16 transactivator target in the Mediator. Milbradt AG., Kulkarni M., Yi T., Takeuchi K., Sun ZY., Luna RE., Selenko P., Näär AM., Wagner G. Only protein-protein interactions imex curation g8hbg2_hhv11 Alpha trans-inducing protein UL48 Alpha-TIF protein protein hhv11 Human herpesvirus 1 (strain 17) (HHV-1) Human herpes simplex virus 1 HHV-1 MDLLVDELFADMNADGASPPPPRPAGGPKNTPAAPPLYATGRLSQAQLMPSPPMPVPPAALFNRLLDDLGFSAGPALCTMLDTWNEDLFSALPTNADLYRECKFLSTLPSDVVEWGDAYVPERTQIDIRAHGDVAFPTLPATRDGLGLYYEALSRFFHAELRAREESYRTVLANFCSALYRYLRASVRQLHRQAHMRGRDRDLGEMLRATIADRYYRETARLARVLFLHLYLFLTREILWAAYAEQMMRPDLFDCLCCDLESWRQLAGLFQPFMFVNGALTVRGVPIEARRLRELNHIREHLNLPLVRSAATEEPGAPLTTPPTLHGNQARASGYFMVLIRAKLDSYSSFTTSPSEAVMREHAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG 8DDDEDEDB2A699D3 med25_human Mediator of RNA polymerase II transcription subunit 25 MED25 ACID1 ARC92 PTOV2 Mediator complex subunit 25 Activator-recruited cofactor 92 kDa component Activator interaction domain-containing protein 1 p78 TCBAP0758 protein protein human Homo sapiens Human MVPGSEGPARAGSVVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQPPTDVSQDPRHMVLVRGLVLPVGGGSAPGPLQSKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPGNLSAAQVAAQNAVEAAKNQKAGLGPRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPGAPKPPPASQPSLVSTVAPGSGLAPTAQPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQQRGMGGQQAPPGLGPILEDQARPSQNLLQLRPPQPQPQGTVGASGATGQPQPQGTAQPPPGAPQGPPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPPAQSWPAQLPPRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI med25-vp16-1 3223175 N/A 3223178 unspecified role unspecified role unspecified role unspecified role Transactivation domain Region 411-452 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3223179 unspecified role unspecified role unspecified role unspecified role VP16-binding domain Region 391-553 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false vp16-med25-2 3223176 N/A 3223178 unspecified role unspecified role bait bait Transactivation domain Region 411-490 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3223179 unspecified role unspecified role prey prey VP16-binding domain Region 391-553 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q71SY5:p.Gln451Glu Q451E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q71SY5:p.His499Glu H499E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q71SY5:p.Lys447Glu K447E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q71SY5:p.Lys545Glu K545E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false med25-vp16-2 3223177 N/A 3223178 unspecified role unspecified role unspecified role unspecified role Transactivation domain Region 411-452 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3223179 unspecified role unspecified role unspecified role unspecified role VP16-binding domain Region 391-553 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q71SY5:p.Gln451Glu Q451E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false