UniProt UniProt http://www.uniprot.org amano-2011-1 human-bosc_23 Homo sapiens embryonic kidney cell line BOSC-23 bosc_23 Human embryonic kidney cell line BOSC-23 The BOSC-23 cell line are a derivative of the 293T cell line. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Amano K., Hata K., Muramatsu S., Wakabayashi M., Takigawa Y., Ono K., Nakanishi M., Takashima R., Kogo M., Matsuda A., Nishimura R., Yoneda T. Origin of Sox9 according PubMed 18677406 following cited Hata et al, 2008, PubMed 19759178 rikonisi@dent.osaka-u.ac.jp rikonis@dent.osaka-u.ac.jp Molecular biology of the cell 2011 imex curation Only protein-protein interactions amano-2011-2 mouse-atdc5 mouse ATDC5 chondrocytic cell line Mouse atdc5 ATDC5 chondrogenic cell line confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Amano K., Hata K., Muramatsu S., Wakabayashi M., Takigawa Y., Ono K., Nakanishi M., Takashima R., Kogo M., Matsuda A., Nishimura R., Yoneda T. Origin of Sox9 according PubMed 18677406 following cited Hata et al, 2008, PubMed 19759178 rikonisi@dent.osaka-u.ac.jp rikonis@dent.osaka-u.ac.jp Molecular biology of the cell 2011 imex curation Only protein-protein interactions amano-2011-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Amano K., Hata K., Muramatsu S., Wakabayashi M., Takigawa Y., Ono K., Nakanishi M., Takashima R., Kogo M., Matsuda A., Nishimura R., Yoneda T. Origin of Sox9 according PubMed 18677406 following cited Hata et al, 2008, PubMed 19759178 rikonisi@dent.osaka-u.ac.jp rikonis@dent.osaka-u.ac.jp Molecular biology of the cell 2011 imex curation Only protein-protein interactions sox9_human Transcription factor SOX-9 SOX9 protein protein human Homo sapiens Human MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTENTRPQENTFPKGEPDLKKESEEDKFPVCIREAVSQVLKGYDWTLVPMPVRVNGSSKNKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPPNGHPGVPATHGQVTYTGSYGISSTAATPASAGHVWMSKQQAPPPPPQQPPQAPPAPQAPPQPQAAPPQQPAAPPQQPQAHTLTTLSSEPGQSQRTHIKTEQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTRP ari5a_mouse AT-rich interactive domain-containing protein 5A Arid5a protein protein mouse Mus musculus Mouse MAAPPAKGNTEQSEEGDLPQLPVSPKPDDEQSRSQSPTQLQDSPEAGGEQEEEQAFLVSLYKFMKERHTPIERVPHLGFKQINLWKIYKAVEKLGAYELVTGRRLWKNVYDELGGSPGSTSAATCTRRHYERLVLPYVRHLKGEDDKPLPPTKPRKQYKMAKELRGDDGTTEKLKKAKDSEERRVEQTTPGKTKSDATGQTQLPCQGSSRDSTEQLGPVSGPSPPLTGASSCPEAYKRLLSSFYCKGAHGIMSPLAKKKLLAQVSKAEALQCQEEGCRHGARSPNKDIQDSPQNLRGPAENSEHQLTPREGLQAPGGSTRMEAQVGPCPTAPMFSGCFHAYPTEVLKPVSQHPRDFFSGLKDRVLLGPPGKEEGPTTKESHLVWGGDANHPSAFHKGSTRKRSFYPKPKACWVSPMAKVPTERPGAPSPHPSSPGLGSKRGLEEEGFAHGGKKLRAVSPFLKEVDSKETGGKPAAPGLAVSCLLGPTPGPTPPEAYRGTMLRCPLNFTGSADPLKGQASLPFSPLVIPAFPAHLLATTGSSPMAASLMHFPPTPYDAVLRNRLGPASSAWHMPPVTTYAAPHFFHLNTKL arid5a-sox9-1 2967275 N/A 2967278 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2967279 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2A arid5a-sox9-2 2967276 N/A 2967278 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection dsred fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2967279 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region required to bind necessary binding region required to bind range range range range false region venus venus fluorescent protein tag venus undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind range range range range false colocalization colocalization false false false 2B, 7C sox9-arid5a-1 2967277 N/A 2967279 unspecified role unspecified role prey prey cell lysate cell lysate region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false region required to bind necessary binding region required to bind range range range range false human-bosc_23 Homo sapiens embryonic kidney cell line BOSC-23 bosc_23 Human embryonic kidney cell line BOSC-23 The BOSC-23 cell line are a derivative of the 293T cell line. N/A 2967278 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 7B