DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ boettcher-2011-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Boettcher AJ., Wu J., Kim C., Yang J., Bruystens J., Cheung N., Pennypacker JK., Blumenthal DA., Kornev AP., Taylor SS. DIP original experiment: DIP-218775X Structure (London, England : 1993) 2011 IMEx Realizing the allosteric potential of the tetrameric protein kinase A RIα holoenzyme. Boettcher AJ., Wu J., Kim C., Yang J., Bruystens J., Cheung N., Pennypacker JK., Blumenthal DA., Kornev AP., Taylor SS. Only protein-protein interactions imex curation boettcher-2011-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Boettcher AJ., Wu J., Kim C., Yang J., Bruystens J., Cheung N., Pennypacker JK., Blumenthal DA., Kornev AP., Taylor SS. DIP original experiment: DIP-218776X Structure (London, England : 1993) 2011 IMEx Realizing the allosteric potential of the tetrameric protein kinase A RIα holoenzyme. Boettcher AJ., Wu J., Kim C., Yang J., Bruystens J., Cheung N., Pennypacker JK., Blumenthal DA., Kornev AP., Taylor SS. Only protein-protein interactions imex curation boettcher-2011-3 in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Boettcher AJ., Wu J., Kim C., Yang J., Bruystens J., Cheung N., Pennypacker JK., Blumenthal DA., Kornev AP., Taylor SS. DIP original experiment: DIP-218777X Structure (London, England : 1993) 2011 IMEx Realizing the allosteric potential of the tetrameric protein kinase A RIα holoenzyme. Boettcher AJ., Wu J., Kim C., Yang J., Bruystens J., Cheung N., Pennypacker JK., Blumenthal DA., Kornev AP., Taylor SS. Only protein-protein interactions imex curation kap0_bovin cAMP-dependent protein kinase type I-alpha regulatory subunit PRKAR1A protein protein bovin Bos taurus (Bovine) Bovine MASGTTASEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFEKLEKEEAKQIQNLQKAGSRADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV kapca_mouse cAMP-dependent protein kinase catalytic subunit alpha Prkaca Pkaca protein protein mouse Mus musculus Mouse MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF prkaca-prkar1a-6 3222440 N/A 3222443 unspecified role unspecified role unspecified role unspecified role Region 74-245 Region 74-245 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3222444 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false prkaca-prkar1a-7 3222441 N/A 3222443 unspecified role unspecified role unspecified role unspecified role Region 74-245 Region 74-245 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3222444 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false prkaca-prkar1a-8 3222442 N/A 3222443 unspecified role unspecified role unspecified role unspecified role Region 2-260 Region 2-260 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3222444 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false