DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ tang-2011-6 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. DIP original experiment: DIP-218744X Structure (London, England : 1993) 2011 IMEx Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation. Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. Only protein-protein interactions imex curation tang-2011-7 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. DIP original experiment: DIP-218751X Structure (London, England : 1993) 2011 IMEx Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation. Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. Only protein-protein interactions imex curation tang-2011-8 ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. DIP original experiment: DIP-218753X Structure (London, England : 1993) 2011 IMEx Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation. Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. Only protein-protein interactions imex curation tang-2011-9 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. DIP original experiment: DIP-218749X Structure (London, England : 1993) 2011 IMEx Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation. Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. Only protein-protein interactions imex curation tang-2011-10 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. DIP original experiment: DIP-218752X Structure (London, England : 1993) 2011 IMEx Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation. Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. Only protein-protein interactions imex curation tang-2011-11 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. DIP original experiment: DIP-218748X Structure (London, England : 1993) 2011 IMEx Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation. Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. Only protein-protein interactions imex curation tang-2011-12 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. DIP original experiment: DIP-218745X Structure (London, England : 1993) 2011 IMEx Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation. Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. Only protein-protein interactions imex curation tang-2011-13 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. DIP original experiment: DIP-218747X Structure (London, England : 1993) 2011 IMEx Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation. Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. Only protein-protein interactions imex curation tang-2011-14 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap anti tag western anti tag western blot anti tag western Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. DIP original experiment: DIP-218750X Structure (London, England : 1993) 2011 IMEx Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation. Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. Only protein-protein interactions imex curation tang-2011-15 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. DIP original experiment: DIP-218746X Structure (London, England : 1993) 2011 IMEx Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation. Tang Y., Holbert MA., Delgoshaie N., Wurtele H., Guillemette B., Meeth K., Yuan H., Drogaris P., Lee EH., Durette C., Thibault P., Verreault A., Cole PA., Marmorstein R. Only protein-protein interactions imex curation vps75_yeast Vacuolar protein sorting-associated protein 75 N0890 YNL246W VPS75 protein protein yeast Saccharomyces cerevisiae Baker's yeast MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKIVLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQVTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIFGWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLEDEEGESGLSADGDSEDDDGSLGEVDLPLSDEEPSSKKRKV 779F12CEA27B3E6B rt109_yeast Histone acetyltransferase RTT109 RTT109 KIM2 REM50 Regulator of Ty1 transposition protein 109 L1377 YLL002W KAT11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGKVFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYLQKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFTCPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQAKLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEEDRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRAIKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLAITMLKPRKKAKALPKT 17825D6EF97C4BB5 msi1_yeast Chromatin assembly factor 1 subunit p50 MSI1 CAC3 YBR195C YBR1405 Protein MSI1 IRA1 multicopy suppressor protein protein yeast Saccharomyces cerevisiae Baker's yeast MNQCAKDITHEASSIPIDLQERYSHWKKNTKLLYDYLNTNSTKWPSLTCQFFPDLDTTSDEHRILLSSFTSSQKPEDETIYISKISTLGHIKWSSLNNFDMDEMEFKPENSTRFPSKHLVNDISIFFPNGECNRARYLPQNPDIIAGASSDGAIYIFDRTKHGSTRIRQSKISHPFETKLFGSHGVIQDVEAMDTSSADINEATSLAWNLQQEALLLSSHSNGQVQVWDIKQYSHENPIIDLPLVSINSDGTAVNDVTWMPTHDSLFAACTEGNAVSLLDLRTKKEKLQSNREKHDGGVNSCRFNYKNSLILASADSNGRLNLWDIRNMNKSPIATMEHGTSVSTLEWSPNFDTVLATAGQEDGLVKLWDTSCEETIFTHGGHMLGVNDISWDAHDPWLMCSVANDNSVHIWKPAGNLVGHS 0D3DB6CB2AC74166 rlf2_yeast Chromatin assembly factor 1 subunit p90 RLF2 CAC1 YPR018W YP9531.12 CAF-1 90 kDa subunit RAP1 localization factor 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEQHLKSIPLQDDTKKKGILSFFQNTTTVKSNKFLTKEKDVITLDDPKEDVSGPMIETVKQETMKSINKECADEMKTTPKKANAEDKLLCYKNSPIQSTKYDRNTNKQVPNGNIIAIETKSRSSSPCSKRELSSSKKEEAKREKELKKQQRAEEKHRKELLRQEEKKKKELKVEEERQRRAELKKQKEEEKRRKEEARLEAKRRKEEERLKKEEEIRLKEEAKERAQSRIGNFFKKLSDSNTPVVEKSDYEKFFLPFYAKDGVRVSNKWKLTKVELEGSKRKIDDELLNSKDKTSSDDLLNWLQSRRLPRGHKIKRKAVDVLQQMPLKEKTDDELQSLLAQVPHKYIKFYENVRPPFIGTYSMDFTLPPNDPFSTKGTGFNYDYDSDVEWVNEEEEGEVDNLESGEEEEEEEDDEDVPSEGEFDGFLDSEENSDLDGLPCAKRKFVGPLIPTICLKSNFENLSEENKRYLQQLKAEVIIETDGPIDPFKEPKTSSLPSKRSNSDLQAQTASQSQSPEKKQKAMITDPMDLLRLFDGVQDSTFSLGTVTEIAQKNLPQYNKQTIKNTIKEYAIRSSGKGDLPRKWVIKDAQNWENLRANANMPTPSL C593E6E4FE5FD778 cac2_yeast Chromatin assembly factor 1 subunit p60 CAC2 CAF-1 60 kDa subunit YML102W protein protein yeast Saccharomyces cerevisiae Baker's yeast MEASHLQIYWHDSQPVYSLTFQKNSANDKLFTAGGDNKVRIWKLNRDENGQNGGVRKIESLDFLGSLTHHEQAINVIRFNSKGDVLASAGDDGQVLLWKQEDPNTQQESVVRPFGMDAETSEADENKEKWVVWKRLRGGSGATAAAEIYDLAWSPDNRNIVVACMDNSIRLFDVGAGMLVCGQSDHGHYVQGVAWDPLNQFILSQSADRSLHVYGVILSSAGVVTGLKLRSKIAKAELPCPGDVLRTNYLFHNETLPSFFRRCSISPCGGLVVIPSGVYKVAGDEVANCVYVYTRSGILNSAGGVKNRPAIRIPSLKKPALMAAFSPVFYETCQKSVLKLPYKLVFAIATTNEVLVYDTDVLEPLCVVGNIHYSPITDLAWSEDGSTLLISSTDGFCSYVSIDTETQFGSRIEPPAMHAEPLDTDESAVAAKNQREAGGIVNMLPVKKIPCNSSDSKKRRIHPTPVDL F24853C4AA4D97C2 h4_yeast Histone H4 N2752 YBR0122 YBR009C YNL030W HHF1 HHF2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGRGKGGKGLGKGGAKRHRKILRDNIQGITKPAIRRLARRGGVKRISGLIYEEVRAVLKSFLESVIRDSVTYTEHAKRKTVTSLDVVYALKRQGRTLYGFGG 69B7D1F89E62DE41 h3_yeast Histone H3 SIN2 YNL031C N2749 HHT1 YBR010W YBR0201 HHT2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MARTKQTARKSTGGKAPRKQLASKAARKSAPSTGGVKKPHRYKPGTVALREIRRFQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVEAYLVSLFEDTNLAAIHAKRVTIQKKDIKLARRLRGERS A6115FEB480AC67A rtt109-vps75-10 3221288 N/A 3221298 unspecified role unspecified role unspecified role unspecified role Core domain Region 1-232 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3221299 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false cac2-cac1-1 3221289 N/A 3221300 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221301 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221302 unspecified role unspecified role bait bait TAP-tag TAP-tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221303 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221304 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221298 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221299 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association false false false rtt109-vps75-11 3221290 N/A 3221298 unspecified role unspecified role unspecified role unspecified role Core domain Region 1-232 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3221299 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rtt109-vps75-12 3221291 N/A 3221298 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221299 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q07794:p.[Glu378Arg;Asn382Arg] E378R/N382R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q07794:p.Leu148Asp L148D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false cac2-cac1-2 3221292 N/A 3221300 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221301 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221302 unspecified role unspecified role bait bait TAP-tag TAP-tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221303 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221304 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221298 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221299 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association false false false rtt109-vps75-13 3221293 N/A 3221298 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3221299 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q07794:p.Leu148Asp L148D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q07794:p.[Ile150Asp;Leu151Asp] I150D/L151D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q07794:p.[Leu148Asp;Ile150Asp;Leu151Asp] L148D/I150D/L151D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q07794:p.[Glu378Arg;Asn382Arg] E378R/N382R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q07794:p.[Arg355Glu;Lys356Glu] R355E/K356E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rtt109-vps75-14 3221294 N/A 3221298 unspecified role unspecified role unspecified role unspecified role Core domain Region 1-232 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3221299 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rtt109-vps75-15 3221295 N/A 3221298 unspecified role unspecified role prey prey P53853:p.[Arg73Asp;Ala74Asp] R73D/A74D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P53853:p.Ala19Asp A19D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P53853:p.[Cys21Glu;Val25Ser;Ile28Glu;Val32Glu] C21E/V25S/I28E/V32E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P53853:p.[Arg173Glu;Lys177Glu] R173E/K177E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P53853:p.[Glu218Lys;Asp222Lys] E218K/D222K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3221299 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false rtt109-vps75-16 3221296 N/A 3221298 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P53853:p.[Arg173Glu;Lys177Glu] R173E/K177E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P53853:p.[Glu218Lys;Asp222Lys] E218K/D222K mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3221299 unspecified role unspecified role bait bait TAP-tag TAP-tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false h3-rtt109-1 3221297 N/A 3221304 unspecified role unspecified role unspecified role unspecified role Region 2-15 Region 2-15 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3221298 unspecified role unspecified role unspecified role unspecified role Core domain Region 1-232 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3221299 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false