DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ pei-2011-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pei XY., Hinchliffe P., Symmons MF., Koronakis E., Benz R., Hughes C., Koronakis V. DIP original experiment: DIP-249114X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structures of sequential open states in a symmetrical opening transition of the TolC exit duct. Pei XY., Hinchliffe P., Symmons MF., Koronakis E., Benz R., Hughes C., Koronakis V. Only protein-protein interactions imex curation pei-2011-2 ecolx Escherichia coli crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Pei XY., Hinchliffe P., Symmons MF., Koronakis E., Benz R., Hughes C., Koronakis V. DIP original experiment: DIP-249115X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structures of sequential open states in a symmetrical opening transition of the TolC exit duct. Pei XY., Hinchliffe P., Symmons MF., Koronakis E., Benz R., Hughes C., Koronakis V. Only protein-protein interactions imex curation pei-2011-3 ecolx Escherichia coli crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Pei XY., Hinchliffe P., Symmons MF., Koronakis E., Benz R., Hughes C., Koronakis V. DIP original experiment: DIP-249116X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structures of sequential open states in a symmetrical opening transition of the TolC exit duct. Pei XY., Hinchliffe P., Symmons MF., Koronakis E., Benz R., Hughes C., Koronakis V. Only protein-protein interactions imex curation tolc_ecoli Outer membrane protein TolC tolC mtcB mukA refI Multidrug efflux pump subunit TolC Outer membrane factor TolC colE1-i toc weeA JW5503 b3035 protein protein ecoli Escherichia coli (strain K12) MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAADRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSKWRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLDQTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAALNVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASTGISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGASEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIVDVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPENVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN tolc-3 3221181 N/A 3221184 unspecified role unspecified role unspecified role unspecified role Region 23-450 Region 23-450 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tolc-4 3221182 N/A 3221184 unspecified role unspecified role unspecified role unspecified role P02930:p.[Ala181Cys;Ser372Cys] A181C/S372C mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tolc-5 3221183 N/A 3221184 unspecified role unspecified role unspecified role unspecified role P02930:p.Asp396Cys D396C mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false