DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lazarus-2011-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lazarus MB., Nam Y., Jiang J., Sliz P., Walker S. DIP original experiment: DIP-219056X Nature 2011 IMEx Structure of human O-GlcNAc transferase and its complex with a peptide substrate. Lazarus MB., Nam Y., Jiang J., Sliz P., Walker S. Only protein-protein interactions imex curation lazarus-2011-2 in vitro In vitro glycosylase assay glycosylase assay predetermined predetermined participant predetermined Lazarus MB., Nam Y., Jiang J., Sliz P., Walker S. DIP original experiment: DIP-219057X Nature 2011 IMEx Structure of human O-GlcNAc transferase and its complex with a peptide substrate. Lazarus MB., Nam Y., Jiang J., Sliz P., Walker S. Only protein-protein interactions imex curation ckii3k CKII3K peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KKKYPGGSTPVSSANMM o15294-3 UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit O-GlcNAc transferase subunit p110 O-linked N-acetylglucosamine transferase 110 kDa subunit OGT protein protein human Homo sapiens Human MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPHHSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKNRQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPVEVTESA ckii3k-ogt-1 3220843 N/A 3220845 unspecified role unspecified role unspecified role unspecified role Region 4-17 Region 4-17 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3220846 unspecified role unspecified role unspecified role unspecified role Region 313-1031 Region 313-1031 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ckii3k-ogt-2 3220844 N/A 3220845 enzyme target enzyme target substrate substrate unspecified role unspecified role in vitro In vitro N/A 3220846 enzyme enzyme unspecified role unspecified role Region 313-1031 Region 313-1031 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false O15294-3:p.His498Ala H498A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O15294-3:p.His558Ala H558A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O15294-3:p.His901Ala H901A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O15294-3:p.His499Ala H499A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli glycosylation glycosylation reaction glycosylation false false false