DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ marcinowski-2011-1 in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Marcinowski M., Höller M., Feige MJ., Baerend D., Lamb DC., Buchner J. DIP original experiment: DIP-255397X Nature structural & molecular biology 2011 IMEx Substrate discrimination of the chaperone BiP by autonomous and cochaperone-regulated conformational transitions. Marcinowski M., Höller M., Feige MJ., Baerend D., Lamb DC., Buchner J. Only protein-protein interactions imex curation marcinowski-2011-2 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Marcinowski M., Höller M., Feige MJ., Baerend D., Lamb DC., Buchner J. DIP original experiment: DIP-255400X Nature structural & molecular biology 2011 IMEx Substrate discrimination of the chaperone BiP by autonomous and cochaperone-regulated conformational transitions. Marcinowski M., Höller M., Feige MJ., Baerend D., Lamb DC., Buchner J. Only protein-protein interactions imex curation marcinowski-2011-3 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Marcinowski M., Höller M., Feige MJ., Baerend D., Lamb DC., Buchner J. DIP original experiment: DIP-255398X Nature structural & molecular biology 2011 IMEx Substrate discrimination of the chaperone BiP by autonomous and cochaperone-regulated conformational transitions. Marcinowski M., Höller M., Feige MJ., Baerend D., Lamb DC., Buchner J. Only protein-protein interactions imex curation marcinowski-2011-4 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Marcinowski M., Höller M., Feige MJ., Baerend D., Lamb DC., Buchner J. DIP original experiment: DIP-255399X Nature structural & molecular biology 2011 IMEx Substrate discrimination of the chaperone BiP by autonomous and cochaperone-regulated conformational transitions. Marcinowski M., Höller M., Feige MJ., Baerend D., Lamb DC., Buchner J. Only protein-protein interactions imex curation djb11_mouse DnaJ homolog subfamily B member 11 Dnajb11 ER-associated Hsp40 co-chaperone ER-associated DNAJ APOBEC1-binding protein 2 Endoplasmic reticulum DNA J domain-containing protein 3 protein protein mouse Mus musculus Mouse MAPQNLSTFCLLLLYLIGTVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQSSHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHRIFERRGDDLYTNVTVSLVEALVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAKEGIKQLLKQGPVQKVYNGLQGY mint bip_mouse Endoplasmic reticulum chaperone BiP Immunoglobulin heavy chain-binding protein Hspa5 Grp78 78 kDa glucose-regulated protein Binding-immunoglobulin protein Heat shock protein 70 family protein 5 Heat shock protein family A member 5 protein protein mouse Mus musculus Mouse MMKFTVVAAALLLLGAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSGGPPPTGEEDTSEKDEL dnajb11-hspa5-2 3220409 N/A 3220413 unspecified role unspecified role unspecified role unspecified role Q99KV1:p.His53Gln H53Q mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3220414 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false dnajb11-hspa5-3 3220410 N/A 3220413 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli N/A 3220414 unspecified role unspecified role fret pair fluorescence acceptor donor pair fret pair Atto532-label Atto532-label atto 532 atto 532 certain certain sequence position certain certain certain sequence position certain false Atto647-label Atto647-label atto 647 atto 647 certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dnajb11-hspa5-4 3220411 N/A 3220413 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3220414 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false dnajb11-hspa5-5 3220412 N/A 3220413 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli N/A 3220414 unspecified role unspecified role fret pair fluorescence acceptor donor pair fret pair Atto532-label Atto532-label atto 532 atto 532 certain certain sequence position certain certain certain sequence position certain false Atto647-label Atto647-label atto 647 atto 647 certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false