DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lombana-2010-1 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Lombana TN., Echols N., Good MC., Thomsen ND., Ng HL., Greenstein AE., Falick AM., King DS., Alber T. DIP original experiment: DIP-217481X Structure (London, England : 1993) 2010 IMEx Allosteric activation mechanism of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknB. Lombana TN., Echols N., Good MC., Thomsen ND., Ng HL., Greenstein AE., Falick AM., King DS., Alber T. Only protein-protein interactions imex curation lombana-2010-2 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lombana TN., Echols N., Good MC., Thomsen ND., Ng HL., Greenstein AE., Falick AM., King DS., Alber T. DIP original experiment: DIP-217482X Structure (London, England : 1993) 2010 IMEx Allosteric activation mechanism of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknB. Lombana TN., Echols N., Good MC., Thomsen ND., Ng HL., Greenstein AE., Falick AM., King DS., Alber T. Only protein-protein interactions imex curation pknb_myctu Serine/threonine-protein kinase PknB pknB MTCY10H4.14c Rv0014c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTTPSHLSDRYELGEILGFGGMSEVHLARDLRLHRDVAVKVLRADLARDPSFYLRFRREAQNAAALNHPAIVAVYDTGEAETPAGPLPYIVMEYVDGVTLRDIVHTEGPMTPKRAIEVIADACQALNFSHQNGIIHRDVKPANIMISATNAVKVMDFGIARAIADSGNSVTQTAAVIGTAQYLSPEQARGDSVDARSDVYSLGCVLYEVLTGEPPFTGDSPVSVAYQHVREDPIPPSARHEGLSADLDAVVLKALAKNPENRYQTAAEMRADLVRVHNGEPPEAPKVLTDAERTSLLSSAAGNLSGPRTDPLPRQDLDDTDRDRSIGSVGRWVAVVAVLAVLTVVVTIAINTFGGITRDVQVPDVRGQSSADAIATLQNRGFKIRTLQKPDSTIPPDHVIGTDPAANTSVSAGDEITVNVSTGPEQREIPDVSTLTYAEAVKKLTAAGFGRFKQANSPSTPELVGKVIGTNPPANQTSAITNVVIIIVGSGPATKDIPDVAGQTVDVAQKNLNVYGFTKFSQASVDSPRPAGEVTGTNPPAGTTVPVDSVIELQVSKGNQFVMPDLSGMFWVDAEPRLRALGWTGMLDKGADVDAGGSQHNRVVYQNPPAGTGVNRDGIITLRFGQ 6C27EEBE9D5A453B pknb-pknb-2 3218932 N/A 3218934 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli N/A 3218934 unspecified role unspecified role neutral component neutral component fkb tag tag tag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation false false false pknb-pknb-3 3218933 N/A 3218934 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-308 Region 1-308 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P9WI81:p.Asp138Asn D138N mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3218934 enzyme enzyme unspecified role unspecified role Kinase domain Region 1-330 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false