DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ pennell-2010-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Pennell S., Westcott S., Ortiz-Lombardía M., Patel D., Li J., Nott TJ., Mohammed D., Buxton RS., Yaffe MB., Verma C., Smerdon SJ. DIP original experiment: DIP-218306X Structure (London, England : 1993) 2010 IMEx Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. Pennell S., Westcott S., Ortiz-Lombardía M., Patel D., Li J., Nott TJ., Mohammed D., Buxton RS., Yaffe MB., Verma C., Smerdon SJ. Only protein-protein interactions imex curation pennell-2010-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Pennell S., Westcott S., Ortiz-Lombardía M., Patel D., Li J., Nott TJ., Mohammed D., Buxton RS., Yaffe MB., Verma C., Smerdon SJ. DIP original experiment: DIP-218308X Structure (London, England : 1993) 2010 IMEx Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. Pennell S., Westcott S., Ortiz-Lombardía M., Patel D., Li J., Nott TJ., Mohammed D., Buxton RS., Yaffe MB., Verma C., Smerdon SJ. Only protein-protein interactions imex curation pennell-2010-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pennell S., Westcott S., Ortiz-Lombardía M., Patel D., Li J., Nott TJ., Mohammed D., Buxton RS., Yaffe MB., Verma C., Smerdon SJ. DIP original experiment: DIP-218307X Structure (London, England : 1993) 2010 IMEx Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. Pennell S., Westcott S., Ortiz-Lombardía M., Patel D., Li J., Nott TJ., Mohammed D., Buxton RS., Yaffe MB., Verma C., Smerdon SJ. Only protein-protein interactions imex curation pthr Optimal peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) DTAPTEKIAYKK fhaa_myctu FHA domain-containing protein FhaA fhaA TB39.8 Rv0020c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MGSQKRLVQRVERKLEQTVGDAFARIFGGSIVPQEVEALLRREAADGIQSLQGNRLLAPNEYIITLGVHDFEKLGADPELKSTGFARDLADYIQEQGWQTYGDVVVRFEQSSNLHTGQFRARGTVNPDVETHPPVIDCARPQSNHAFGAEPGVAPMSDNSSYRGGQGQGRPDEYYDDRYARPQEDPRGGPDPQGGSDPRGGYPPETGGYPPQPGYPRPRHPDQGDYPEQIGYPDQGGYPEQRGYPEQRGYPDQRGYQDQGRGYPDQGQGGYPPPYEQRPPVSPGPAAGYGAPGYDQGYRQSGGYGPSPGGGQPGYGGYGEYGRGPARHEEGSYVPSGPPGPPEQRPAYPDQGGYDQGYQQGATTYGRQDYGGGADYTRYTESPRVPGYAPQGGGYAEPAGRDYDYGQSGAPDYGQPAPGGYSGYGQGGYGSAGTSVTLQLDDGSGRTYQLREGSNIIGRGQDAQFRLPDTGVSRRHLEIRWDGQVALLADLNSTNGTTVNNAPVQEWQLADGDVIRLGHSEIIVRMH 2642F9CF23FE7906 fha-pthr-1 3218796 N/A 3218799 unspecified role unspecified role unspecified role unspecified role pThr pThr opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false pthr:p.Thr5Ser T5S/Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Region 1-8 Region 1-8 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false pthr:p.Thr5Tyr mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3218800 unspecified role unspecified role unspecified role unspecified role FHA domain Region 430-527 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fha-pthr-2 3218797 N/A 3218799 unspecified role unspecified role unspecified role unspecified role pThr pThr opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false Region 1-8 Region 1-8 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3218800 unspecified role unspecified role unspecified role unspecified role FHA domain Region 430-527 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P71590:p.Ser473Ala S473A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P71590:p.Asn495Ala N495A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P71590:p.Arg459Ala R459A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fha-pthr-3 3218798 N/A 3218799 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3218800 unspecified role unspecified role unspecified role unspecified role FHA domain Region 430-527 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false