DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ shi-2010-26 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Shi H., Blobel G. DIP original experiment: DIP-220662X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx UNC-45/CRO1/She4p (UCS) protein forms elongated dimer and joins two myosin heads near their actin binding region. Shi H., Blobel G. Only protein-protein interactions imex curation shi-2010-27 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Shi H., Blobel G. DIP original experiment: DIP-220663X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx UNC-45/CRO1/She4p (UCS) protein forms elongated dimer and joins two myosin heads near their actin binding region. Shi H., Blobel G. Only protein-protein interactions imex curation shi-2010-28 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Shi H., Blobel G. DIP original experiment: DIP-220666X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx UNC-45/CRO1/She4p (UCS) protein forms elongated dimer and joins two myosin heads near their actin binding region. Shi H., Blobel G. Only protein-protein interactions imex curation shi-2010-29 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Shi H., Blobel G. DIP original experiment: DIP-220664X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx UNC-45/CRO1/She4p (UCS) protein forms elongated dimer and joins two myosin heads near their actin binding region. Shi H., Blobel G. Only protein-protein interactions imex curation shi-2010-30 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Shi H., Blobel G. DIP original experiment: DIP-220665X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx UNC-45/CRO1/She4p (UCS) protein forms elongated dimer and joins two myosin heads near their actin binding region. Shi H., Blobel G. Only protein-protein interactions imex curation shi-2010-31 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Shi H., Blobel G. DIP original experiment: DIP-220667X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx UNC-45/CRO1/She4p (UCS) protein forms elongated dimer and joins two myosin heads near their actin binding region. Shi H., Blobel G. Only protein-protein interactions imex curation she4_yeast SWI5-dependent HO expression protein 4 OR26.26 YOR035C SHE4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPLCEKGNDPIDSSTIDSLCAAFDKTLKSTPDVQKYNDAINTIFQLRQKSESGKMPADLTNSEALKDRQKIEEILTRSYQDHSESRVHLSKLIQNDIPFALNLFEILSRSSIHVFVGCFSNKDATIALLNELQIRIHYGEDTHVTYLLSIILQLLNKFKYNFKEVRFLVKELILRISEDEVKSMMLIIFAELQSSFQKDFDKAVVDFMSSLIVEAEIDVGNDPLSIIVKTLSELYPSLTTLCSEIFLTKGLSKLFKKRVFEEQDLQFTKELLRLLSSACIDETMRTYITENYLQLLERSLNVEDVQIYSALVLVKTWSFTKLTCINLKQLSEIFINAISRRIMPKIENVNESAVKLEEVPKVEMSVEALAYLSLKASVKIMIRSNESFTEILLTMIKSQKMTHCLYGLLVIMANLSTLPEESNGSSQSINDLKNYADLKGPGADKVGAEKESKEDILLFNEKYILRTELISFLKREMHNLSPNCKQQVVRVIYNITRSKNFIPQCISQGGTTIILEYLANKQDIGEPIRILGCRALTRMLIFTNPGLIFKKYSALNAIPFLFELLPRSTPVDDNPLHNDEQIKLTDNYEALLALTNLASSETSDGEEVCKHIVSTKVYWSTIENLMLDENVPLQRSTLELISNMMSHPLTIAAKFFNLENPQSLRNFNILVKLLQLSDVESQRAVAAIFANIATTIPLIAKELLTKKELIENAIQVFADQIDDIELRQRLLMLFFGLFEVIPDNGTNEVYPLLQENQKLKDALNMSLKRGDSGPEFSAAIPVILAKIKV 4A0E1C7D41939B44 myo4_yeast Myosin-4 MYO4 SHE1 YAL029C FUN22 SWI5-dependent HO expression protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFEVGTKCWYPHKEQGWIGGEVTKNDFFEGTFHLELKLEDGETVSIETNSFENDDDHPTLPVLRNPPILESTDDLTTLSYLNEPAVLHAIKKRYMNGQIYTYSGIVLIAANPFDKVDHLYSREMIQNYSSKRKDELEPHLFAIAEEAYRFMVHEKANQTVVVSGESGAGKTVSAKYIMRYFASVQESNNREGEVEMSQIESQILATNPIMEAFGNAKTTRNDNSSRFGKYLQILFDENTTIRGSKIRTYLLEKSRLVYQPETERNYHIFYQILEGLPEPVKQELHLSSPKDYHYTNQGGQPNIAGIDEAREYKITTDALSLVGINHETQLGIFKILAGLLHIGNIEMKMTRNDASLSSEEQNLQIACELLGIDPFNFAKWIVKKQIVTRSEKIVTNLNYNQALIARDSVAKFIYSTLFDWLVDNINKTLYDPELDQQDHVFSFIGILDIYGFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYVKEEIEWSFIEFSDNQPCIDLIENKLGILSLLDEESRLPSGSDESWASKLYSAFNKPPSNEVFSKPRFGQTKFIVSHYAVDVEYEVEGFIEKNRDSVSLGHLDVFKATTNPIFKQILDNRELRSDDAPEEQNTEKKIMIPARLSQKKPTLGSMFKKSLGELMAIINSTNVHYIRCIKPNSEKKPWEFDNLMVLSQLRACGVLETIRISCAGFPSRWTFDEFVQRYFLLTDYSLWSGILYNPDLPKEAIVNFCQSILDATISDSAKYQIGNTKIFFKAGMLAFLEKLRTNKMNEICIIIQKKIRARYYRLQYLQTMESIKKCQSQIRSLLVRTRVDHELKTRAAILLQTNIRALWKREYYRAAIGQIIKLQCTCKRKLILDSVNRKFMLMAAVIIQSYIRSYGHKTDYRTLKRSSILVQSAMRMQLARRRYIVLQKEVEERNIRASYGIGLLEEAIEFKNSFILNLEMLNDSYTRLTQLLQGDLSNIPSKQRQEYETIVNGYNDKISKLKTLQVEIMNTLNKKNALKERKKKQSSLIQSHMQSLAAIKGNKPSRLSDEVKSMKQELAFIENVIAQDFTTTYSANKNDKVKGLGIAGQQVKPKLVNVIRRESGNPDLLELLMDLNCYTLEVTEGYLKKVNVTEVNGDNVLGPIHVITTVVSSLVRNGLLIQSSKFISKVLLTVESIVMSLPKDETMLGGIFWLSNLSRLPAFAANQKTLYEANGGDEKDKLTLIYLNDLENETLKVFDKIYSTWLVKFMKHASAHIEIFDMVLNEKLFKNSGDEKFAKLFTFLNEFDAVLCKFQVVDSMHTKIFNDTLKYLNVMLFNDLITKCPALNWKYGYEVDRNIERLVSWFEPRIEDVRPNLIQIIQAVKILQLKISNLNEFKLLFDFWYALNPAQIQAILLKYKPANKGEAGVPNEILNYLANVIKRENLSLPGKMEIMLSAQFDSAKNHLRYDTSAITQNSNTEGLATVSKIIKLDRK E79C0FE72B041E95 she4p-1 3218509 N/A 3218515 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false she4p-2 3218510 N/A 3218515 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false myo4p-she4p-1 3218511 N/A 3218516 unspecified role unspecified role bait bait Region 561-587 Region 561-587 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3218515 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false she4p-3 3218512 N/A 3218515 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false myo4p-she4p-2 3218513 N/A 3218516 unspecified role unspecified role unspecified role unspecified role Region 561-587 Region 561-587 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3218515 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false myo4p-she4p-3 3218514 N/A 3218516 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 561-587 Region 561-587 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3218515 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false Deletion of region 545-789 decreased the interaction rate/strength.