DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ huynh-2010-1 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Huynh TN., Noriega CE., Stewart V. DIP original experiment: DIP-220631X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. Huynh TN., Noriega CE., Stewart V. Only protein-protein interactions imex curation huynh-2010-2 in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase predetermined predetermined participant predetermined Huynh TN., Noriega CE., Stewart V. DIP original experiment: DIP-220633X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. Huynh TN., Noriega CE., Stewart V. Only protein-protein interactions imex curation huynh-2010-3 in vitro In vitro protein phosphatase assay protein phosphatase assay predetermined predetermined participant predetermined Huynh TN., Noriega CE., Stewart V. DIP original experiment: DIP-220632X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. Huynh TN., Noriega CE., Stewart V. Only protein-protein interactions imex curation narx_ecoli Nitrate/nitrite sensor protein NarX narX narR b1222 JW1213 protein protein ecoli Escherichia coli (strain K12) MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGLQDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMAVFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQNLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRLADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGDCRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE narl_ecoli Nitrate/nitrite response regulator protein NarL narL frdR b1221 JW1212 protein protein ecoli Escherichia coli (strain K12) MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQGIELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRGADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRERDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF narx-2 3217801 N/A 3217804 putative self putative self putative self putative self P0AFA2:p.Gln404Ala Q404A/N/E mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.Asp400Glu D400E/N mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.Gln404Asn mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.Asp400Asn mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.Gln404Glu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false true false narl-narx-1 3217802 N/A 3217805 phosphate acceptor phosphate acceptor unspecified role unspecified role ecolx Escherichia coli N/A 3217804 phosphate donor phosphate donor unspecified role unspecified role ecolx Escherichia coli phosphotransfer phosphotransfer reaction phosphotransfer false false false narl-narx-2 3217803 N/A 3217805 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3217804 enzyme enzyme unspecified role unspecified role P0AFA2:p.Gln404Ala Q404A/E mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.Gln404His D404H/N mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.Asp400Ala D400A/S mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.[His399Gln;Gln404His] Q404H/H399Q mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.His399Gln H399Q mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.[His399Gln;Asp400Ala] D400A/H399Q mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.[His399Gln;Gln404Ala] Q404A/H399Q mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.Gln404Asn D404H/N mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.Asp400Ser mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false P0AFA2:p.Gln404Glu mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation false false false