DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yim-2010-1 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yim H., Erikson RL. DIP original experiment: DIP-220571X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Yim H., Erikson RL. Only protein-protein interactions imex curation yim-2010-2 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yim H., Erikson RL. DIP original experiment: DIP-220569X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Yim H., Erikson RL. Only protein-protein interactions imex curation yim-2010-3 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yim H., Erikson RL. DIP original experiment: DIP-220573X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Yim H., Erikson RL. Only protein-protein interactions imex curation yim-2010-4 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Yim H., Erikson RL. DIP original experiment: DIP-220575X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Yim H., Erikson RL. Only protein-protein interactions imex curation yim-2010-5 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Yim H., Erikson RL. DIP original experiment: DIP-220574X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Yim H., Erikson RL. Only protein-protein interactions imex curation yim-2010-6 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Yim H., Erikson RL. DIP original experiment: DIP-220576X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Yim H., Erikson RL. Only protein-protein interactions imex curation yim-2010-7 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yim H., Erikson RL. DIP original experiment: DIP-220572X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Yim H., Erikson RL. Only protein-protein interactions imex curation yim-2010-8 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yim H., Erikson RL. DIP original experiment: DIP-220570X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Yim H., Erikson RL. Only protein-protein interactions imex curation yim-2010-9 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Yim H., Erikson RL. DIP original experiment: DIP-220577X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Yim H., Erikson RL. Only protein-protein interactions imex curation cdk1_human Cyclin-dependent kinase 1 p34 protein kinase Cell division protein kinase 1 CDK1 CDC2 Cell division control protein 2 homolog CDC28A CDKN1 P34CDC2 protein protein human Homo sapiens Human MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM cdc6_human Cell division control protein 6 homolog CDC6 CDC18L CDC6-related protein p62(cdc6) Cdc18-related protein protein protein human Homo sapiens Human MPQTRSQAQATISFPKRKLSRALNKAKNSSDAKLEPTNVQTVTCSPRVKALPLSPRKRLGDDNLCNTPHLPPCSPPKQGKKENGPPHSHTLKGRRLVFDNQLTIKSPSKRELAKVHQNKILSSVRKSQEITTNSEQRCPLKKESACVRLFKQEGTCYQQAKLVLNTAVPDRLPAREREMDVIRNFLREHICGKKAGSLYLSGAPGTGKTACLSRILQDLKKELKGFKTIMLNCMSLRTAQAVFPAIAQEICQEEVSRPAGKDMMRKLEKHMTAEKGPMIVLVLDEMDQLDSKGQDVLYTLFEWPWLSNSHLVLIGIANTLDLTDRILPRLQAREKCKPQLLNFPPYTRNQIVTILQDRLNQVSRDQVLDNAAVQFCARKVSAVSGDVRKALDVCRRAIEIVESDVKSQTILKPLSECKSPSEPLIPKRVGLIHISQVISEVDGNRMTLSQEGAQDSFPLQQKILVCSLMLLIRQLKIKEVTLGKLYEAYSKVCRKQQVAAVDQSECLSLSGLLEARGILGLKRNKETRLTKVFFKIEEKEIEHALKDKALIGNILATGLP plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS cdc6-cdk1-1 3217043 N/A 3217052 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3217053 unspecified role unspecified role bait bait Q99741:p.Thr37Val T37V mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false cdc6-plk1-1 3217044 N/A 3217054 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false C-terminal domain Region 306-603 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P53350:p.[Trp414Phe;Val415Ala;Leu427Ala] W414F/V415A/L427A/ mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3217053 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false cdc6-plk1-2 3217045 N/A 3217054 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3217053 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false cdc6-plk1-3 3217046 N/A 3217054 enzyme enzyme unspecified role unspecified role P53350:p.Lys82Met K82M mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3217053 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false cdc6-plk1-4 3217047 N/A 3217054 unspecified role unspecified role prey prey C-terminal domain Region 306-603 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3217054 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3217053 unspecified role unspecified role bait bait human Homo sapiens Human association association false false false cdc6-plk1-5 3217048 N/A 3217054 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3217053 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-100 Region 1-100 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q99741:p.Thr37Val T37V mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false cdc6-cdk1-2 3217049 N/A 3217052 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3217053 unspecified role unspecified role bait bait Q99741:p.Thr37Val T37V mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false plk1-cdc6-1 3217050 N/A 3217054 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3217053 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false plk1-1 3217051 N/A 3217054 putative self putative self unspecified role unspecified role P53350:p.Lys82Met K82M mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false