DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ feld-2010-1 in vitro In vitro ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217709X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-2 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217714X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-3 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217721X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-4 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217717X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-5 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217718X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217710X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-7 in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217715X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-8 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217711X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-9 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217719X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-10 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217716X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-11 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217722X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-12 in vitro In vitro ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217712X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-13 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217720X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-14 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217723X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation feld-2010-15 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. DIP original experiment: DIP-217713X Nature structural & molecular biology 2010 IMEx Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers. Feld GK., Thoren KL., Kintzer AF., Sterling HJ., Tang II., Greenberg SG., Williams ER., Krantz BA. Only protein-protein interactions imex curation lef_bacan Lethal factor Anthrax lethal toxin endopeptidase component lef pXO1-107 BXA0172 GBAA_pXO1_0172 protein protein bacan Bacillus anthracis MNIKKEFIKVISMSCLVTAITLSGPVFIPLVQGAGGHGDVGMHVKEKEKNKDENKRKDEERNKTQEEHLKEIMKHIVKIEVKGEEAVKKEAAEKLLEKVPSDVLEMYKAIGGKIYIVDGDITKHISLEALSEDKKKIKDIYGKDALLHEHYVYAKEGYEPVLVIQSSEDYVENTEKALNVYYEIGKILSRDILSKINQPYQKFLDVLNTIKNASDSDGQDLLFTNQLKEHPTDFSVEFLEQNSNEVQEVFAKAFAYYIEPQHRDVLQLYAPEAFNYMDKFNEQEINLSLEELKDQRMLARYEKWEKIKQHYQHWSDSLSEEGRGLLKKLQIPIEPKKDDIIHSLSQEEKELLKRIQIDSSDFLSTEEKEFLKKLQIDIRDSLSEEEKELLNRIQVDSSNPLSEKEKEFLKKLKLDIQPYDINQRLQDTGGLIDSPSINLDVRKQYKRDIQNIDALLHQSIGSTLYNKIYLYENMNINNLTATLGADLVDSTDNTKINRGIFNEFKKNFKYSISSNYMIVDINERPALDNERLKWRIQLSPDTRAGYLENGKLILQRNIGLEIKDVQIIKQSEKEYIRIDAKVVPKSKIDTKIQEAQLNINQEWNKALGLPKYTKLITFNVHNRYASNIVESAYLILNEWKNNIQSDLIKKVTNYLVDGNGRFVFTDITLPNIAEQYTHQDEIYEQVHSKGLYVPESRSILLHGPSKGVELRNDSEGFIHEFGHAVDDYAGYLLDKNQSDLVTNSKKFIDIFKEEGSNLTSYGRTNEAEFFAEAFRLMHSTDHAERLKVQKNAPKTFQFINDQIKFIINS pag_bacan Protective antigen PA-83 Anthrax toxins translocating protein pagA pag pXO1-110 BXA0164 GBAA_pXO1_0164 protein protein bacan Bacillus anthracis MKKRKVLIPLMALSTILVSSTGNLEVIQAEVKQENRLLNESESSSQGLLGYYFSDLNFQAPMVVTSSTTGDLSIPSSELENIPSENQYFQSAIWSGFIKVKKSDEYTFATSADNHVTMWVDDQEVINKASNSNKIRLEKGRLYQIKIQYQRENPTEKGLDFKLYWTDSQNKKEVISSDNLQLPELKQKSSNSRKKRSTSAGPTVPDRDNDGIPDSLEVEGYTVDVKNKRTFLSPWISNIHEKKGLTKYKSSPEKWSTASDPYSDFEKVTGRIDKNVSPEARHPLVAAYPIVHVDMENIILSKNEDQSTQNTDSQTRTISKNTSTSRTHTSEVHGNAEVHASFFDIGGSVSAGFSNSNSSTVAIDHSLSLAGERTWAETMGLNTADTARLNANIRYVNTGTAPIYNVLPTTSLVLGKNQTLATIKAKENQLSQILAPNNYYPSKNLAPIALNAQDDFSSTPITMNYNQFLELEKTKQLRLDTDQVYGNIATYNFENGRVRVDTGSNWSEVLPQIQETTARIIFNGKDLNLVERRIAAVNPSDPLETTKPDMTLKEALKIAFGFNEPNGNLQYQGKDITEFDFNFDQQTSQNIKNQLAELNATNIYTVLDKIKLNAKMNILIRDKRFHYDRNNIAVGADESVVKEAHREVINSSTEGLLLNIDKDIRKILSGYIVEIEDTEGLKEVINDRYDMLNISSLRQDGKTFIDFKKYNDKLPLYISNPNYKVNVYAVTKENTIINPSENGDTSTNGIKKILIFSKKGYEIG lef-paga-6 3216861 N/A 3216876 unspecified role unspecified role unspecified role unspecified role PA-binding domain Region 34-296 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false paga-8 3216862 N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lef-paga-7 3216863 N/A 3216876 unspecified role unspecified role unspecified role unspecified role PA-binding domain Region 34-296 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false IAEDANS-label IAEDANS-label iaedans label iaedans label IAEDANS 1,5-IAEDANS (5((((2-iodoacetyl)amino)ethyl)amino)-naphthalene-1-sulfonic acid) IAEDANS 1,5-IAEDANS certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false paga-9 3216864 N/A 3216877 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Alexa647-label Alexa647-label alexa label alexa label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216877 unspecified role unspecified role fluorescence donor fluorescence donor Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Alexa555-label Alexa555-label alexa label alexa label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lef-paga-8 3216865 N/A 3216876 unspecified role unspecified role unspecified role unspecified role PA-binding domain Region 34-296 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false IAEDANS-label IAEDANS-label iaedans label iaedans label IAEDANS 1,5-IAEDANS (5((((2-iodoacetyl)amino)ethyl)amino)-naphthalene-1-sulfonic acid) IAEDANS 1,5-IAEDANS certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lef-paga-9 3216866 N/A 3216876 unspecified role unspecified role unspecified role unspecified role PA-binding domain Region 34-296 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lef-paga-10 3216867 N/A 3216876 unspecified role unspecified role unspecified role unspecified role PA-binding domain Region 34-296 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P13423:p.Leu216Ala L216A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P13423:p.Arg229Ser R229S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P13423:p.His240Ala H240A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P13423:p.Pro234Ser P234S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P13423:p.Ile236Ser I236S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lef-paga-11 3216868 N/A 3216876 unspecified role unspecified role unspecified role unspecified role PA-binding domain Region 34-296 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lef-paga-12 3216869 N/A 3216876 unspecified role unspecified role unspecified role unspecified role PA-binding domain Region 34-296 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false IAEDANS-label IAEDANS-label iaedans label iaedans label IAEDANS 1,5-IAEDANS (5((((2-iodoacetyl)amino)ethyl)amino)-naphthalene-1-sulfonic acid) IAEDANS 1,5-IAEDANS certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false paga-lef-2 3216870 N/A 3216877 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Alexa647-label Alexa647-label alexa label alexa label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216876 unspecified role unspecified role neutral component neutral component PA-binding domain Region 34-296 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216877 unspecified role unspecified role fluorescence donor fluorescence donor Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Alexa555-label Alexa555-label alexa label alexa label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lef-paga-13 3216871 N/A 3216876 unspecified role unspecified role unspecified role unspecified role PA-binding domain Region 34-296 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false IAEDANS-label IAEDANS-label iaedans label iaedans label IAEDANS 1,5-IAEDANS (5((((2-iodoacetyl)amino)ethyl)amino)-naphthalene-1-sulfonic acid) IAEDANS 1,5-IAEDANS certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false paga-10 3216872 N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lef-paga-14 3216873 N/A 3216876 unspecified role unspecified role unspecified role unspecified role PA-binding domain Region 34-296 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false IAEDANS-label IAEDANS-label iaedans label iaedans label IAEDANS 1,5-IAEDANS (5((((2-iodoacetyl)amino)ethyl)amino)-naphthalene-1-sulfonic acid) IAEDANS 1,5-IAEDANS certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lef-paga-15 3216874 N/A 3216876 unspecified role unspecified role unspecified role unspecified role PA-binding domain Region 34-296 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false IAEDANS-label IAEDANS-label iaedans label iaedans label IAEDANS 1,5-IAEDANS (5((((2-iodoacetyl)amino)ethyl)amino)-naphthalene-1-sulfonic acid) IAEDANS 1,5-IAEDANS certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false paga-11 3216875 N/A 3216877 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false