DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ saito-2010-1 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. DIP original experiment: DIP-220533X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Omnipotent role of archaeal elongation factor 1 alpha (EF1α in translational elongation and termination, and quality control of protein synthesis. Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. Only protein-protein interactions imex curation saito-2010-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. DIP original experiment: DIP-220537X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Omnipotent role of archaeal elongation factor 1 alpha (EF1α in translational elongation and termination, and quality control of protein synthesis. Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. Only protein-protein interactions imex curation saito-2010-3 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. DIP original experiment: DIP-220534X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Omnipotent role of archaeal elongation factor 1 alpha (EF1α in translational elongation and termination, and quality control of protein synthesis. Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. Only protein-protein interactions imex curation saito-2010-4 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. DIP original experiment: DIP-220539X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Omnipotent role of archaeal elongation factor 1 alpha (EF1α in translational elongation and termination, and quality control of protein synthesis. Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. Only protein-protein interactions imex curation saito-2010-5 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. DIP original experiment: DIP-220536X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Omnipotent role of archaeal elongation factor 1 alpha (EF1α in translational elongation and termination, and quality control of protein synthesis. Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. Only protein-protein interactions imex curation saito-2010-6 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. DIP original experiment: DIP-220538X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Omnipotent role of archaeal elongation factor 1 alpha (EF1α in translational elongation and termination, and quality control of protein synthesis. Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. Only protein-protein interactions imex curation saito-2010-7 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. DIP original experiment: DIP-220535X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Omnipotent role of archaeal elongation factor 1 alpha (EF1α in translational elongation and termination, and quality control of protein synthesis. Saito K., Kobayashi K., Wada M., Kikuno I., Takusagawa A., Mochizuki M., Uchiumi T., Ishitani R., Nureki O., Ito K. Only protein-protein interactions imex curation ef1a_aerpe Elongation factor 1-alpha tuf Elongation factor Tu APE_1844 protein protein aerpe Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) MAEKPHMNLVVIGHVDHGKSTLVGHLLYRLGYIEEKKLKELEEQAKSRGKESFKFAWILDKMKEERERGITIDLTFMKFETKKYVFTIIDAPGHRDFVKNMITGASQADAAILVVSARKGEFEAGMSTEGQTREHLLLARTMGIEQIIVAVNKMDAPDVNYDQKRYEFVVSVLKKFMKGLGYQVDKIPFIPVSAWKGDNLIERSPNMPWYNGPTLVEALDQLQPPAKPVDKPLRIPVQNVYSIPGAGTVPVGRVETGVLRVGDKVVFMPPGVVGEVRSIEMHYQQLQQAEPGDNIGFAVRGVSKSDIKRGDVAGHLDKPPTVAEEFEARIFVIWHPSAITVGYTPVIHVHTASVSSRIIEIKAKLDPKTGQVVEQNPQFLKAGDAAIVRFKPVKPLVVEKFSEIPQLGRFAMRDMNRTVGIGIVTDVKPAKVDIKAK D6AE87FEB8AD003C rf1_aerpe Peptide chain release factor subunit 1 Translation termination factor aRF1 APE_1988.1 prf1 protein protein aerpe Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) MSQGRLEERLTISKRELARLLKELKKWSAPATVLLSLYIPPGRPLSDVMTLLRQEYSITDNIKLKRTRQAVKRALSAAMDRLQMLTSTPPNGLVLFCGEDMSTGKFECFMFSPPEPIRVFYYRTDKRFITDFLEDMVEDNNAIGIIIVERDQATIGLLKGARLEVLKELEGFVPGKHKMGGQSQRRYERIIEQMVDEFFKKVGEEASNLLVPLAEKGVLKGVIVAGPGLAKQEFVEGNYLDYRLKKILAPELVDVAYQGLQGLKEAVMKAEKVVEAQMYRDAVNAMEEFKLHLAKGTGMIVYGEKDVEAALEMGAVKTLLIHESREDLEEWVEKAKSSGAQVIVVPESLAEAEWFLKTFGGLAGILRFRISTV pelo_aerpe Protein pelota homolog pelA APE_1800.1 protein protein aerpe Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) MRVEVLDNKRRIVRLRPESEEDLWLLRITLRPGDVVRIRTSRDVPVGSGRKERVVMTLRIRLDSIEFQPFTGKLRISGIVVEGPDEFGVKGRRHSTAVSIGTWLVVERDKGWSEQELERLASGRARGTAVIAAVDYDEFALAVLAGHGMKILEDTSARLPGKDDPSREQEVEKYVDRAAKRIVEEAARHRSPIAVIAGPGQLKTSVAEKVQRAMPSLKVATVDTSMGGVAGVREALRRESVTRILRELSIVEAEGVLEEFLRRIAKSRDTVAYTPGEVLAVARMGAVDTVLLVDTLLHSPDDAVREAVDEALRLVESMGGRVIIIPGDSPAGERLVSFGGVIALLRYPVPQEARRL 389A5D27B7752986 erf1_schpo Eukaryotic peptide chain release factor subunit 1 sup45 SPAC1834.01 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSETAEKAIEIWKIRRLVKQLINCHGNGTSMITLIIPPGEQISRYSNMLAEEYGTASNIKSRVNRLSVLSAITSTRERLKLYNKVPDNGLVIYCGEVIMEGNKTRKLNIDFEPFKPINTSQYLCDNKFHTEALAELLESDQRFGFIVMDGHQTLYGVVSGSAREVLQRFTVDLPKKHGRGGQSALRFARLRDEKRHNYVRKVAEGAVQHFITDDKPNVAGIVLAGSADFKTELGQSDLFDQRLQSRIIKTVDVSYGGDAGFNQAIELAADTLSNVKYVQEKKLIQRFFDEISLDSGKYCFGVVDTMNALQEGAVETLLCFADLDMIRYEFKNSEGNPVITYMTKEQEEKDSTNSFLLDKDTGAEMELVSSMLLSEWLAEHYKDYGANLEFVSDRSQEGMQFVKGFGGIGAVMRYQLDLSMLDPESDEFYSDSD 5648B6EA2FD90997 erf3_schpo Eukaryotic peptide chain release factor GTP-binding subunit sup35 ERF-3 ERF2 Polypeptide release factor 3 Translation release factor 3 SPCC584.04 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MASNQPNNGEQDEQLAKQTSKLSMSAKAPTFTPKAAPFIPSFQRPGFVPVNNIAGGYPYAQYTGQGQNSNSPHPTKSYQQYYQKPTGNTVDEDKSRVPDFSKKKSFVPPKPAIPKGKVLSLGGNTSAPKSTKPISISLGGTKAPTTTKPAAPAAQSKTETPAPKVTSESTKKETAAPPPQETPTKSADAELAKTPSAPAAALKKAAEAAEPATVTEDATDLQNEVDQELLKDMYGKEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYKECVDKLSMFLRRVAGYNSKTDVKYMPVSAYTGQNVKDRVDSSVCPWYQGPSLLEYLDSMTHLERKVNAPFIMPIASKYKDLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDDSDVQTGYVLTSTKNPVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDKTNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 54AE41EA6AF6168A tuf-prf1-1 3216741 N/A 3216748 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3216749 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false tuf-prf1-2 3216742 N/A 3216748 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3216749 unspecified role unspecified role prey prey Q9YAF1:p.Glu168Ala E168A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9YAF1:p.Gly228Ala G228A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9YAF1:p.Lys290Ala K290A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9YAF1:p.Leu293Ala L293A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9YAF1:p.Trp354Ala W354A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9YAF1:p.Phe359Ala F359A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false pela-tuf-1 3216743 N/A 3216750 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3216748 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false erf3-erf1-1 3216744 N/A 3216751 unspecified role unspecified role prey prey P79063:p.Leu173Ala L173A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P79063:p.Lys176Ala K176A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Region 143-433 Region 143-433 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3216752 unspecified role unspecified role bait bait Region 196-662 Region 196-662 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false tuf-pela-1 3216745 N/A 3216750 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3216748 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false erf3-erf1-2 3216746 N/A 3216751 unspecified role unspecified role prey prey Region 143-433 Region 143-433 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3216752 unspecified role unspecified role bait bait O74718:p.Thr294Ala T294A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O74718:p.Glu297Ala E297A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O74718:p.Glu305Ala E305A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O74718:p.Tyr329Ala Y329A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O74718:p.Tyr477Ala Y477A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Region 196-662 Region 196-662 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false tuf-prf1-3 3216747 N/A 3216748 unspecified role unspecified role bait bait Q9YAV0:p.Glu64Ala E64A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9YAV0:p.Glu65Ala E65A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9YAV0:p.Asp73Ala D73A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9YAV0:p.Ser337Ala S337A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9YAV0:p.Ala338Ser A338S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9YAV0:p.Phe379Ala F379A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3216749 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false