DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ gttler-2010-1 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. DIP original experiment: DIP-218370X Nature structural & molecular biology 2010 IMEx NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. Only protein-protein interactions imex curation gttler-2010-2 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. DIP original experiment: DIP-218376X Nature structural & molecular biology 2010 IMEx NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. Only protein-protein interactions imex curation gttler-2010-3 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. DIP original experiment: DIP-218372X Nature structural & molecular biology 2010 IMEx NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. Only protein-protein interactions imex curation gttler-2010-4 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. DIP original experiment: DIP-218379X Nature structural & molecular biology 2010 IMEx NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. Only protein-protein interactions imex curation gttler-2010-5 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. DIP original experiment: DIP-218374X Nature structural & molecular biology 2010 IMEx NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. Only protein-protein interactions imex curation gttler-2010-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. DIP original experiment: DIP-218371X Nature structural & molecular biology 2010 IMEx NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. Only protein-protein interactions imex curation gttler-2010-7 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. DIP original experiment: DIP-218377X Nature structural & molecular biology 2010 IMEx NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. Only protein-protein interactions imex curation gttler-2010-8 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. DIP original experiment: DIP-218378X Nature structural & molecular biology 2010 IMEx NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. Only protein-protein interactions imex curation gttler-2010-9 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. DIP original experiment: DIP-218375X Nature structural & molecular biology 2010 IMEx NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. Only protein-protein interactions imex curation gttler-2010-10 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. DIP original experiment: DIP-218373X Nature structural & molecular biology 2010 IMEx NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M., Görlich D. Only protein-protein interactions imex curation ipka_human cAMP-dependent protein kinase inhibitor alpha PRKACN1 cAMP-dependent protein kinase inhibitor, muscle/brain isoform PKIA protein protein human Homo sapiens Human MTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEEDAQRSSTEQSGEAQGEAAKSES xpo1_mouse Exportin-1 Xpo1 Crm1 Chromosome region maintenance 1 protein homolog protein protein mouse Mus musculus Mouse MPAIMTMLADHAARQLLDFSQKLDINLLDNVVNCLYHGEGAQQRMAQEVLTHLKEHPDAWTRVDTILEFSQNMNTKYYGLQILENVIKTRWKILPRNQCEGIKKYVVGLIIKTSSDPTCVEKEKVYIGKLNMILVQILKQEWPKHWPTFISDIVGASRTSESLCQNNMVILKLLSEEVFDFSSGQITQVKAKHLKDSMCNEFSQIFQLCQFVMENSQNAPLVHATLETLLRFLNWIPLGYIFETKLISTLIYKFLNVPMFRNVSLKCLTEIAGVSVSQYEEQFETLFTLTMMQLKQMLPLNTNIRLAYSNGKDDEQNFIQNLSLFLCTFLKEHGQLLEKRLNLREALMEALHYMLLVSEVEETEIFKICLEYWNHLAAELYRESPFSTSASPLLSGSQHFDIPPRRQLYLTVLSKVRLLMVSRMAKPEEVLVVENDQGEVVREFMKDTDSINLYKNMRETLVYLTHLDYVDTEIIMTKKLQNQVNGTEWSWKNLNTLCWAIGSISGAMHEEDEKRFLVTVIKDLLGLCEQKRGKDNKAIIASNIMYIVGQYPRFLRAHWKFLKTVVNKLFEFMHETHDGVQDMACDTFIKIAQKCRRHFVQVQVGEVMPFIDEILNNINTIICDLQPQQVHTFYEAVGYMIGAQTDQTVQEHLIEKYMLLPNQVWDSIIQQATKNVDILKDPETVKQLGSILKTNVRACKAVGHPFVIQLGRIYLDMLNVYKCLSENISAAIQANGEMVTKQPLIRSMRTVKRETLKLISGWVSRSNDPQMVAENFVPPLLDAVLIDYQRNVPAAREPEVLSTMAIIVNKLGGHITAEIPQIFDAVFECTLNMINKDFEEYPEHRTNFFLLLQAVNSHCFPAFLAIPPAQFKLVLDSIIWAFKHTMRNVADTGLQILFTLLQNVAQEEAAAQSFYQTYFCDILQHIFSVVTDTSHTAGLTMHASILAYMFNLVEEGKISTPLNPGNPVNNQMFIQDYVANLLKSAFPHLQDAQVKLFVTGLFSLNQDIPAFKEHLRDFLVQIKEFAGEDTSDLFLEERETALRQAQEEKHKLQMSVPGILNPHEIPEEMCD rev_hv1h2 Protein Rev rev ART/TRS Anti-repression transactivator Regulator of expression of viral proteins protein protein hv1h2 Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1) HIV-1 MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRRWRERQRQIHSISERILGTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILVESPTVLESGTKE ran_human GTP-binding nuclear protein Ran GTPase Ran Ras-related nuclear protein Ras-like protein TC4 Androgen receptor-associated protein 24 RAN ARA24 OK/SW-cl.81 protein protein human Homo sapiens Human MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL ddx3_xenla Putative ATP-dependent RNA helicase an3 an3 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSHVAVENVLNLDQQFAGLDLNSADAESGVAGTKGRYIPPHLRNKEASRNDSNWDSGRGGNGYINGMQDDRDGRMNGYDRGGYGSRGTGRSDRGFYDRENSGWNSGRDKDAYSSFGSRGDRGKGSLFNERGSGSRRTDDRRQDGFDGMGNRSDKSGFGRFDRGNSRWSDDRNDEDDWSKPLAPNDRVEQELFSGSNTGINFEKYDDIPVEATGSNCPPHIESFHDVTMGEIIMGNIQLTRYTRPTPVQKHAIPIIIEKRDLMACAQTGSGKTAAFLLPILSQIYADGPGDAMKHLQENGRYGRRKQFPLSLVLAPTRELAVQIYEEARKFAYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRQTMMFSATFPKEIQILARDFLDEYIFLAVGRVGSTSENITQKVVWVEEMDKRSFLLDLLNATGKDSLTLVFVETKKGADALEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNEKNINITKDLLDLLVEAKQEVPSWLENMAYEQHHKSSSRGRSKSRFSGGFGAKDYRQSSGAGSSFGSSRGGRSSGHGGSRGFGGGYGGFYNSDGYGGNYGGSSQVDWWGN s1nes Synthetic peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) DVSDLARLFSALSV q83414_mumiv protein protein mvm Murine minute virus MVM Murine parvovirus MAGNAYSDEVLGTTNWLKEKSNQEVFSFVFKTEDVQLNGKDIGWNNYKKELQEDELKSLQRGAETTWDQSEDMEWESTVDEMTKKFGTLTIHDTEKYASQPELCNNSTCIGSRGPGFRALEHTKYSCCGHCRNPEHWGSWFQSLPRWSTEPNLVRDRGGFESVLRCGTVEERLQRAAELGLRPEITWF spn1_human Snurportin-1 RNA U transporter 1 SNUPN RNUT1 SPN1 protein protein human Homo sapiens Human MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVNHARRLAEDDWTGMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLGVAVPAGPLTTKPDYAGHQLQQIMEHKKSQKEGMKEKLTHKASENGHYELEHLSTPKLKGSSHSPDHPGCLMEN pkia-xpo1-2 3216525 N/A 3216535 unspecified role unspecified role bait bait NES domain Region 35-47 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P61925:p.Ser35Leu S35L mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216536 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false hivrevnes-xpo1-1 3216526 N/A 3216537 unspecified role unspecified role bait bait NES domain Region 72-83 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216536 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false pkia-ran-1 3216527 N/A 3216538 unspecified role unspecified role unspecified role unspecified role Region 1-180 Region 1-180 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216535 unspecified role unspecified role unspecified role unspecified role NES domain Region 35-47 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3216536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false an3-xpo1-1 3216528 N/A 3216539 unspecified role unspecified role bait bait NES domain Region 8-21 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216536 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false pkia-xpo1-3 3216529 N/A 3216535 unspecified role unspecified role unspecified role unspecified role NES domain Region 30-54 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pkia-ran-2 3216530 N/A 3216538 unspecified role unspecified role unspecified role unspecified role Region 1-180 Region 1-180 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216535 unspecified role unspecified role unspecified role unspecified role NES domain Region 35-47 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false s1nes-xpo1-1 3216531 N/A 3216540 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3216536 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false q83414_mumiv-xpo1-1 3216532 N/A 3216541 unspecified role unspecified role bait bait NES domain Region 78-91 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216536 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false snupn-xpo1-8 3216533 N/A 3216542 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3216536 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false ran-xpo1-2 3216534 N/A 3216538 unspecified role unspecified role unspecified role unspecified role Region 1-180 Region 1-180 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false