DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lees-2010-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Lees JA., Yip CK., Walz T., Hughson FM. DIP original experiment: DIP-217694X Nature structural & molecular biology 2010 IMEx Molecular organization of the COG vesicle tethering complex. Lees JA., Yip CK., Walz T., Hughson FM. Only protein-protein interactions imex curation lees-2010-2 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Lees JA., Yip CK., Walz T., Hughson FM. DIP original experiment: DIP-217697X Nature structural & molecular biology 2010 IMEx Molecular organization of the COG vesicle tethering complex. Lees JA., Yip CK., Walz T., Hughson FM. Only protein-protein interactions imex curation lees-2010-3 ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Lees JA., Yip CK., Walz T., Hughson FM. DIP original experiment: DIP-217705X Nature structural & molecular biology 2010 IMEx Molecular organization of the COG vesicle tethering complex. Lees JA., Yip CK., Walz T., Hughson FM. Only protein-protein interactions imex curation lees-2010-4 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Lees JA., Yip CK., Walz T., Hughson FM. DIP original experiment: DIP-217699X Nature structural & molecular biology 2010 IMEx Molecular organization of the COG vesicle tethering complex. Lees JA., Yip CK., Walz T., Hughson FM. Only protein-protein interactions imex curation lees-2010-5 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Lees JA., Yip CK., Walz T., Hughson FM. DIP original experiment: DIP-217702X Nature structural & molecular biology 2010 IMEx Molecular organization of the COG vesicle tethering complex. Lees JA., Yip CK., Walz T., Hughson FM. Only protein-protein interactions imex curation lees-2010-6 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Lees JA., Yip CK., Walz T., Hughson FM. DIP original experiment: DIP-217696X Nature structural & molecular biology 2010 IMEx Molecular organization of the COG vesicle tethering complex. Lees JA., Yip CK., Walz T., Hughson FM. Only protein-protein interactions imex curation lees-2010-7 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lees JA., Yip CK., Walz T., Hughson FM. DIP original experiment: DIP-217703X Nature structural & molecular biology 2010 IMEx Molecular organization of the COG vesicle tethering complex. Lees JA., Yip CK., Walz T., Hughson FM. Only protein-protein interactions imex curation lees-2010-8 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Lees JA., Yip CK., Walz T., Hughson FM. DIP original experiment: DIP-217698X Nature structural & molecular biology 2010 IMEx Molecular organization of the COG vesicle tethering complex. Lees JA., Yip CK., Walz T., Hughson FM. Only protein-protein interactions imex curation lees-2010-9 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Lees JA., Yip CK., Walz T., Hughson FM. DIP original experiment: DIP-217695X Nature structural & molecular biology 2010 IMEx Molecular organization of the COG vesicle tethering complex. Lees JA., Yip CK., Walz T., Hughson FM. Only protein-protein interactions imex curation lees-2010-10 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Lees JA., Yip CK., Walz T., Hughson FM. DIP original experiment: DIP-217700X Nature structural & molecular biology 2010 IMEx Molecular organization of the COG vesicle tethering complex. Lees JA., Yip CK., Walz T., Hughson FM. Only protein-protein interactions imex curation lees-2010-11 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Lees JA., Yip CK., Walz T., Hughson FM. DIP original experiment: DIP-217701X Nature structural & molecular biology 2010 IMEx Molecular organization of the COG vesicle tethering complex. Lees JA., Yip CK., Walz T., Hughson FM. Only protein-protein interactions imex curation lees-2010-12 ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Lees JA., Yip CK., Walz T., Hughson FM. DIP original experiment: DIP-217704X Nature structural & molecular biology 2010 IMEx Molecular organization of the COG vesicle tethering complex. Lees JA., Yip CK., Walz T., Hughson FM. Only protein-protein interactions imex curation cog8_yeast Conserved oligomeric Golgi complex subunit 8 YML071C COG8 DOR1 Component of oligomeric Golgi complex 8 protein protein yeast Saccharomyces cerevisiae Baker's yeast MELILNSLISDDLTEEQKRLSLDFLQDILQSNTKDYESYFSSRAVPGSITEDIAEIDAELSALDRKIRKTLLDNTSQIIGNILENDDRAQLDDIAKSLEQLWELDTNINKAADRNVTNDDINNESVSIDDFLEDDKEDNDTGRIMTTESNNLARKKKEDEFHKALSRLRNRISTKEDDKDDIRSDTLVTVLENLDSITDLMELPFLARTCIRTGHYQEAVMLYTHTTSLRSRFPGSTIVDEVCEKVLNEISTTMLSGLVKLLSTNVSVNSLKKILQYLNSIPPFDGKTNKSLLSVFLAMRYKFITDEIASYPLDVESSNESLIEMMVKRKIEVLREHVYMSLNVFLKSFLYDTNDLEIPFPEELESTVLRINGTNEEKEIEEKEKETKKEEYQKQDSVANNEEDVTENKSIEDVQEEVQGKVEGEDDGAERKTENEIENETVNKTEDKAEKEKEEEVNTKDNKAEKEEEEINKVEVTPEEPSKSIDNKAEKEEEEINKVEVTPEEPSKKIRTSKRENKIPTNAVMLQFVDKCITYVLKDLTRGLNSIKLSDSVCLQLVYCSFRLCDLNRNYHHLFLKKINDTSLFTTEQLARAIDKRAELASKYIYS 57E34E2DE36664B6 cog4_yeast Conserved oligomeric Golgi complex subunit 4 COG4 SEC38 YPR105C P8283.16 SGF1 Component of oligomeric Golgi complex 4 Protein SEC38 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEGQKSNASWESSIIEGQLSKNLARYTLLLDKLSTLSQIDKLSEVIANDYAKQSKQLNAFVQQSQSSLNKESRKLELQRTNLTTTLTQFHETVATISSSNARAKAIHDDIETVDQERALVNKTLQFVKDVRTLKNNISLAHSALETKDYLVAATAINEIRSLPDKKLIVSEFAKKVVPSSEIPEEPAILIKNWCKELTSLFQEQFMEATRTQDIKELTLMFKMFPMIGQDVLGLDLYSKYVCDIIADESRKIMSNSMENSTKFQGFFSQVLLHLFKIVSTIINDHSKVIATCYGKKHMVHVMEKVEKEADLQASLILDIFMETRKIERTIHDINEWEHSQKNEDVNIDSNQSDIETDGETEKSSIISIHDLALLIMEFSQILQNWSMYSRFYSVKWNEFSDLHPHVLQPPPPIADGKFALKLKQDKVFDEFQVFVLNHLQRSFRNSISLEELPSLNDLITAVPLNDHDNISYPVTSVLDDLILLVRKNLISVVNTGQFKLLASFLNELVKFFQNRFLVKFMQNKFKLLQSKLASNVSLKRYIPKGEEQSATSRSVSPPANKFSPLSRFTFRGAAASALTNIQSNLQAVVAEDEDSILALHHYLIYLNTLYLSKVYVHRLLSIEILEDDSQRILRDNFPFDNDAAQLQNLIINSEKLVLEQTDKLSKWAVKYLFQNILQNRVRNLLGTVFVNSASSNSSTSNQKNVSRDYSAGSNQKNYITSIEDFEDLSQINSFNSKWNQLIIPYKNILHNEAYAELLSVIVDYIVTTLEQRIWTLEFNELGVTKLDRELSLFIGNMCGLNYNLREKFLKLTQIVLLLGLDDDNFDLTTGDIKDDFNGTFDWVINSQERIKARNMKIDRTQ 22F265AB2AD91533 cog3_yeast Conserved oligomeric Golgi complex subunit 3 COG3 GRD20 SEC34 Component of oligomeric Golgi complex 3 Protein SEC34 YER157W protein protein yeast Saccharomyces cerevisiae Baker's yeast MARSRKNSLVRDIASHPTIPESQTIVGLLDDSYLFDKLKKLSLAVENSDSLQRTDVSEGCSEVNGSEATTSADVKKTNKYLYYTTYLDQLNIKIDEYKVVLDQTRQVNDQLDSSIKKFRKISQDTGAFIEETKTIYEKQSKLSNLTESIPKALHYFEVLDPIMRRLNHATSPAIVKKSSFTTMLATIDESLRFLDENSDLKDAAAYRIKFKQCLIRACELISHFLTNLLKQTNQEILDKTKNKNSLTGLPSTTRDAFLYSKFYTIADTFKIQVSEIVKRSNEKAYNKYHDELNSILYECFNHYFQTRLRLLTPVIWSHIDEIVVKDKDQGLVKFIQDGKVYFQQLCADEYKLFVEFFPEKECRFKINQWFLQLCEPLYDSIRVRVLKETDICTLCDSVTLFAPYYEFEEGSEEYVKQFTDIQYDKLFEPIVQKVQARLILRVQIYVQQNILSYRPTRDVFMISNRRRKSKTSLQGGNEDATTSDDNPDPLLESYLSSFKNRSILPISPNDADDKSIDSEESTDKISQLQTYYPPLLKTLALLSKIYEMINSVVFDDLAHHVVHDCIVSLRNAYDMVIKSSAGKSDFNNLDISLAYLKNLLMLRDSIQNFNIQYTVNETYLDFSGVEGFFKSLKENGRNVLKKTKSSSILTLARELVPKVVNNMVDARTELISELRNVIKDFTESTSLELIDDTLDINSDEDLLSKNVKLRENIKARLPRIYEQILNYIDDQEIVTNLLDAVQELITQSYSKYYETITELAENGKFAKDQVADVMYLDVFTDFFAKEVADLLRNGDIDTITK C7AFBE99010FE571 cog2_yeast Conserved oligomeric Golgi complex subunit 2 COG2 SEC35 YGR120C G6324 Component of oligomeric Golgi complex 2 Protein SEC35 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDFLNDDELDLDLPVTAEISKELFATEIEKYRESETNGTDVDNFDVDRFLVQKNFHYLPLDSLIRDLSGLSQKMVQTLLEQIRSNYDDYLTFSNTYTDEENETLINLEKTQSDLQKFMTQLDHLIKDDISNTQEIIKDVLEYLKKLDEIYGSLRNHSQLTEALSLGKRLSKSLHEMCGIEPLEEEICSGLIEQLYKLITASRRILESCADSNSPYIHHLRNDYQDLLQEFQISLKILTEKCLENPSSLQNLSLTLVSIIKTA 0ED457A7466F0F53 cog1_yeast Conserved oligomeric Golgi complex subunit 1 COG1 COD3 TFI1 YGL223C SEC36 Component of oligomeric Golgi complex 1 Complexed with DOR1 protein 3 Protein SEC36 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDEVLPLFRDSHIPQIKDYQLELQNDLTKTNEAFQKNLLKNYNKILSLTDSVNDLSLNLKNVDQDFKSLCFNDEKFQLNKLTPLPYQTTTHISPPRDEEKVSIPSQNILVISNWTISINNFCNRIVTSTTPSRIFDELLLNFHELSLIPVPSKFEALVKDKCCRLQKFLVDSMKTLNLTLLQWVKLYNLLNTEFSSKWDDDLLSIFNESLFETLFNDNVQALLISSANSKDHQYHSNQQYKDAIVVDFVNSSTFRDHLIRRTVKEINTHLDTLSTLRAKLKEPETLHKLDIFHDNDTNLNDGTVSPLDDDALKQYIDTAVFYSKGLTNDTTLQIYQTVQPTIEILQNLELYKCPQETLTDLRNKLITQLQEFKTQISSRLPSPLENSTSVVDDFITSYNNHNLLQLVIDQITQLRQQ 65F7EF5B5194D9AB cog1-cog2-5 3216401 N/A 3216413 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216414 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216415 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216416 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216417 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cog1-cog2-6 3216402 N/A 3216414 unspecified role unspecified role unspecified role unspecified role Region 1-553 Region 1-553 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216415 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216416 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216417 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cog2-cog3-3 3216403 N/A 3216414 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216415 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216416 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli association association false false false cog2-cog3-10 3216404 N/A 3216414 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216415 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216416 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cog1-cog2-7 3216405 N/A 3216414 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3216415 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3216416 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3216417 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli association association false false false cog1-cog2-8 3216406 N/A 3216414 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216415 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216416 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216417 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cog1-cog2-9 3216407 N/A 3216414 unspecified role unspecified role unspecified role unspecified role Region 1-553 Region 1-553 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216415 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216416 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216417 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cog2-cog3-11 3216408 N/A 3216414 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216415 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216416 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cog1-cog2-10 3216409 N/A 3216413 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216414 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216415 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216416 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216417 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cog2-cog1-2 3216410 N/A 3216414 unspecified role unspecified role prey prey Region 1-553 Region 1-553 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216415 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3216416 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3216417 unspecified role unspecified role prey prey ecolx Escherichia coli association association false false false cog2-cog3-12 3216411 N/A 3216414 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3216415 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3216416 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli association association false false false cog1-cog2-11 3216412 N/A 3216414 unspecified role unspecified role unspecified role unspecified role Region 1-553 Region 1-553 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216415 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216416 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3216417 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli association association false false false