DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ li-2010-42 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. DIP original experiment: DIP-254124X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Thromboxane A2 receptor and MaxiK-channel intimate interaction supports channel trans-inhibition independent of G-protein activation. Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. Only protein-protein interactions imex curation li-2010-43 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. DIP original experiment: DIP-254129X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Thromboxane A2 receptor and MaxiK-channel intimate interaction supports channel trans-inhibition independent of G-protein activation. Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. Only protein-protein interactions imex curation li-2010-44 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. DIP original experiment: DIP-254131X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Thromboxane A2 receptor and MaxiK-channel intimate interaction supports channel trans-inhibition independent of G-protein activation. Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. Only protein-protein interactions imex curation li-2010-45 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. DIP original experiment: DIP-254132X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Thromboxane A2 receptor and MaxiK-channel intimate interaction supports channel trans-inhibition independent of G-protein activation. Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. Only protein-protein interactions imex curation li-2010-46 rat Rattus norvegicus (Rat) Rat anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. DIP original experiment: DIP-254125X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Thromboxane A2 receptor and MaxiK-channel intimate interaction supports channel trans-inhibition independent of G-protein activation. Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. Only protein-protein interactions imex curation li-2010-47 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. DIP original experiment: DIP-254127X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Thromboxane A2 receptor and MaxiK-channel intimate interaction supports channel trans-inhibition independent of G-protein activation. Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. Only protein-protein interactions imex curation li-2010-48 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. DIP original experiment: DIP-254130X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Thromboxane A2 receptor and MaxiK-channel intimate interaction supports channel trans-inhibition independent of G-protein activation. Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. Only protein-protein interactions imex curation li-2010-49 human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. DIP original experiment: DIP-254126X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Thromboxane A2 receptor and MaxiK-channel intimate interaction supports channel trans-inhibition independent of G-protein activation. Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. Only protein-protein interactions imex curation li-2010-50 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. DIP original experiment: DIP-254128X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Thromboxane A2 receptor and MaxiK-channel intimate interaction supports channel trans-inhibition independent of G-protein activation. Li M., Tanaka Y., Alioua A., Wu Y., Lu R., Kundu P., Sanchez-Pastor E., Marijic J., Stefani E., Toro L. Only protein-protein interactions imex curation kcma1_human Calcium-activated potassium channel subunit alpha-1 KCNMA1 KCNMA SLO Calcium-activated potassium channel, subfamily M subunit alpha-1 Maxi K channel BK channel K(VCA)alpha BKCA alpha KCa1.1 Slowpoke homolog Slo-alpha Slo1 protein protein human Homo sapiens Human MANGGGGGGGSSGGGGGGGGSSLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEAVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRPKMSIYKRMRRACCFDCGRSERDCSCMSGRVRGNVDTLERAFPLSSVSVNDCSTSFRAFEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRQNRPKSRESRDKQKYVQEERL ta2r_human Thromboxane A2 receptor TBXA2R Prostanoid TP receptor protein protein human Homo sapiens Human MWPNGSSLGPCFRPTNITLEERRLIASPWFAASFCVVGLASNLLALSVLAGARQGGSHTRSSFLTFLCGLVLTDFLGLLVTGTIVVSQHAALFEWHAVDPGCRLCRFMGVVMIFFGLSPLLLGAAMASERYLGITRPFSRPAVASQRRAWATVGLVWAAALALGLLPLLGVGRYTVQYPGSWCFLTLGAESGDVAFGLLFSMLGGLSVGLSFLLNTVSVATLCHVYHGQEAAQQRPRDSEVEMMAQLLGIMVVASVCWLPLLVFIAQTVLRNPPAMSPAGQLSRTTEKELLIYLRVATWNQILDPWVYILFRRAVLRRLQPRLSTRPRSLSLQPQLTQRSGLQ p21731-3 Thromboxane A2 receptor Placenta receptor TBXA2R Prostanoid TP receptor Alpha protein protein human Homo sapiens Human MWPNGSSLGPCFRPTNITLEERRLIASPWFAASFCVVGLASNLLALSVLAGARQGGSHTRSSFLTFLCGLVLTDFLGLLVTGTIVVSQHAALFEWHAVDPGCRLCRFMGVVMIFFGLSPLLLGAAMASERYLGITRPFSRPAVASQRRAWATVGLVWAAALALGLLPLLGVGRYTVQYPGSWCFLTLGAESGDVAFGLLFSMLGGLSVGLSFLLNTVSVATLCHVYHGQEAAQQRPRDSEVEMMAQLLGIMVVASVCWLPLLVFIAQTVLRNPPAMSPAGQLSRTTEKELLIYLRVATWNQILDPWVYILFRRAVLRRLQPRLSTRPRSLSLQPQLTQRSGLQ q12791-5 Calcium-activated potassium channel subunit alpha-1 KCNMA1 KCNMA SLO BK channel BKCA alpha Calcium-activated potassium channel, subfamily M subunit alpha-1 K(VCA)alpha KCa1.1 Maxi K channel Slo-alpha Slo1 Slowpoke homolog protein protein human Homo sapiens Human MANGGGGGGGSSGGGGGGGGSSLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEAVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRQNRPKSRESRDKQKYVQEERL kcma1_rat Calcium-activated potassium channel subunit alpha-1 Kcnma1 Kcnma Calcium-activated potassium channel, subfamily M subunit alpha-1 Maxi K channel BK channel K(VCA)alpha BKCA alpha KCa1.1 Slowpoke homolog Slo-alpha Slo1 protein protein rat Rattus norvegicus (Rat) Rat MANGGGGGGGGSSGSSGGGGGGGGGETALRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR ta2r_rat Thromboxane A2 receptor Tbxa2r Prostanoid TP receptor TXR2 protein protein rat Rattus norvegicus (Rat) Rat MWLNSTSLGACFRPVNITLQERRAIASPWFAASFCALGLGSNLLALSVLAGARPGAGPRSSFLALLCGLVLTDFLGLLVTGAVVASQHAALLDWRATDPGCRLCHFMGAAMVFFGLCPLLLGAAMAAERFVGITRPFSRPAATSRRAWATVGLVWVGAGTLGLLPLLGLGRYSVQYPGSWCFLTLGAERGDVAFGLMFALLGSVSVGLSLLLNTVSVATLCRVYHAREATQRPRDCEVEMMVQLVGIMVVATVCWMPLLVFILQTLLQTLPVMSPSGQLLRTTERQLLIYLRVATWNQILDPWVYILFRRSVLRRLHPRFTSQLQAVSLHSPPTQAMLSGP tbxa2r-kcnma1-1 3216189 N/A 3216198 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3216199 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false kcnma1-tbxa2r-1 3216190 N/A 3216200 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3216201 unspecified role unspecified role bait bait Region 66-408 Region 66-408 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human physical association physical association false false false tbxa2r-kcnma1-2 3216191 N/A 3216200 unspecified role unspecified role bait bait ICL1 domain Region 53-66 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3216201 unspecified role unspecified role prey prey Region 66-408 Region 66-408 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Region 387-1178 Region 387-1178 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false tbxa2r-kcnma1-3 3216192 N/A 3216200 unspecified role unspecified role bait bait ICL1 domain Region 53-66 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false C-terminal domain Region 312-343 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3216201 unspecified role unspecified role prey prey Region 66-1178 Region 66-1178 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false tbxa2r-kcnma1-4 3216193 N/A 3216202 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat N/A 3216203 unspecified role unspecified role bait bait rat Rattus norvegicus (Rat) Rat physical association physical association false false false tbxa2r-kcnma1-5 3216194 N/A 3216200 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human N/A 3216201 unspecified role unspecified role prey prey Region 66-1178 Region 66-1178 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false tbxa2r-kcnma1-6 3216195 N/A 3216200 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3216201 unspecified role unspecified role prey prey Region 66-776 Region 66-776 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human physical association physical association false false false kcnma1-tbxa2r-2 3216196 N/A 3216200 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human N/A 3216201 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Region 66-1178 Region 66-1178 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction false false false kcnma1-tbxa2r-3 3216197 N/A 3216200 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human N/A 3216201 unspecified role unspecified role bait bait Region 66-1178 Region 66-1178 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false