DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wang-2010-63 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217684X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-64 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217671X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-65 ecolx Escherichia coli pull down pull down weight by staining molecular weight estimation by staining weight by staining Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217675X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-66 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217681X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-67 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217677X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-68 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217672X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-69 human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217682X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-70 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217678X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-71 ecolx Escherichia coli ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217673X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-72 ecolx Escherichia coli pull down pull down weight by staining molecular weight estimation by staining weight by staining Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217676X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-73 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217679X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-74 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217674X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-75 human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217683X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation wang-2010-76 ecolx Escherichia coli ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. DIP original experiment: DIP-217680X Nature structural & molecular biology 2010 IMEx The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Wang L., Yang JK., Kabaleeswaran V., Rice AJ., Cruz AC., Park AY., Yin Q., Damko E., Jang SB., Raunser S., Robinson CV., Siegel RM., Walz T., Wu H. Only protein-protein interactions imex curation tnr6_mouse Tumor necrosis factor receptor superfamily member 6 Fas Apt1 Tnfrsf6 FASLG receptor Apoptosis-mediating surface antigen FAS Apo-1 antigen protein protein mouse Mus musculus Mouse MLWIWAVLPLVLAGSQLRVHTQGTNSISESLKLRRRVRETDKNCSEGLYQGGPFCCQPCQPGKKKVEDCKMNGGTPTCAPCTEGKEYMDKNHYADKCRRCTLCDEEHGLEVETNCTLTQNTKCKCKPDFYCDSPGCEHCVRCASCEHGTLEPCTATSNTNCRKQSPRNRLWLLTILVLLIPLVFIYRKYRKRKCWKRRQDDPESRTSSRETIPMNASNLSLSKYIPRIAEDMTIQEAKKFARENNIKEGKIDEIMHDSIQDTAEQKVQLLLCWYQSHGKSDAYQDLIKGLKKAECRRTLDKFQDMVQKDLGKSTPDTGNENEGQCLE fadd_human FAS-associated death domain protein FAS-associating death domain-containing protein Mediator of receptor induced toxicity FADD MORT1 GIG3 Growth-inhibiting gene 3 protein protein protein human Homo sapiens Human MDPFLVLLHSVSSSLSSSELTELKFLCLGRVGKRKLERVQSGLDLFSMLLEQNDLEPGHTELLRELLASLRRHDLLRRVDDFEAGAAAGAAPGEEDLCAAFNVICDNVGKDWRRLARQLKVSDTKIDSIEDRYPRNLTERVRESLRIWKNTEKENATVAHLVGALRSCQMNLVADLVQEVQQARDLQNRSGAMSPMSWNSDASTSEAS p25445-1 Tumor necrosis factor receptor superfamily member 6 FAS APT1 FAS1 TNFRSF6 Apo-1 antigen Apoptosis-mediating surface antigen FAS FASLG receptor protein protein human Homo sapiens Human MLGIWTLLPLVLTSVARLSSKSVNAQVTDINSKGLELRKTVTTVETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLTSNTKCKEEGSRSNLGWLCLLLLPIPLIVWVKRKEVQKTCRKHRKENQGSHESPTLNPETVAINLSDVDLSKYITTIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNVQDTAEQKVQLLRNWHQLHGKKEAYDTLIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSLV fadd-fas-8 3215547 N/A 3215561 unspecified role unspecified role unspecified role unspecified role Death domain Region 210-310 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215562 unspecified role unspecified role unspecified role unspecified role Death domain Region 93-184 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fadd-fas-9 3215548 N/A 3215562 unspecified role unspecified role unspecified role unspecified role Death domain Region 93-191 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215563 unspecified role unspecified role unspecified role unspecified role Death domain Region 218-318 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fadd-fas-10 3215549 N/A 3215562 unspecified role unspecified role bait bait Death domain Region 93-191 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3215563 unspecified role unspecified role prey prey Death domain Region 218-318 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Glu261Lys E261K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Glu272Lys E272K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Thr270Lys T270K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Arg250Glu R250E/Q mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Gln283Lys Q283K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Lys287Asp K287D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Asp260Tyr D260Y mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Ala257Asp A257D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Arg250Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Arg250Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false fadd-fas-11 3215550 N/A 3215561 unspecified role unspecified role unspecified role unspecified role Death domain Region 210-310 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215562 unspecified role unspecified role unspecified role unspecified role Death domain Region 93-184 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fadd-fas-12 3215551 N/A 3215561 unspecified role unspecified role unspecified role unspecified role Death domain Region 210-310 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215562 unspecified role unspecified role unspecified role unspecified role Death domain Region 93-184 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fadd-fas-13 3215552 N/A 3215562 unspecified role unspecified role unspecified role unspecified role Death domain Region 93-191 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215563 unspecified role unspecified role unspecified role unspecified role Death domain Region 218-318 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fadd-fas-14 3215553 N/A 3215562 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Death domain Region 93-208 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3215563 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P25445-1:p.Asp260Tyr D260Y/V mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Ala257Asp A257D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Glu261Lys E261K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Arg250Glu R250E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Thr270Lys T270K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Glu272Lys E272K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Gln283Lys Q283K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P25445-1:p.Asp260Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false fas-fadd-3 3215554 N/A 3215561 unspecified role unspecified role bait bait Death domain Region 210-310 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215562 unspecified role unspecified role prey prey Death domain Region 93-184 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false fadd-fas-15 3215555 N/A 3215562 unspecified role unspecified role unspecified role unspecified role Death domain Region 93-191 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215563 unspecified role unspecified role unspecified role unspecified role Death domain Region 218-318 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fadd-fas-16 3215556 N/A 3215562 unspecified role unspecified role bait bait Death domain Region 93-191 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q13158:p.Arg117Glu R117E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13158:p.Asp123Arg D123R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13158:p.Arg135Glu R135E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13158:p.Arg142Glu R142E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13158:p.Leu172Lys L172K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13158:p.Asp175Lys D175K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215563 unspecified role unspecified role prey prey Death domain Region 218-318 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false fadd-fas-17 3215557 N/A 3215561 unspecified role unspecified role unspecified role unspecified role Death domain Region 210-310 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215562 unspecified role unspecified role unspecified role unspecified role Death domain Region 93-184 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fadd-fas-18 3215558 N/A 3215562 unspecified role unspecified role unspecified role unspecified role Death domain Region 93-191 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215563 unspecified role unspecified role unspecified role unspecified role Death domain Region 218-318 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fadd-fas-19 3215559 N/A 3215562 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Death domain Region 93-208 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q13158:p.Arg117Glu R117E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13158:p.Leu172Glu L172E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13158:p.Asp123Arg D123R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13158:p.Arg135Glu R135E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13158:p.Arg142Glu R142E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13158:p.Lys153Glu K153E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3215563 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human direct interaction direct interaction false false false fadd-fas-20 3215560 N/A 3215561 unspecified role unspecified role unspecified role unspecified role Death domain Region 210-310 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215562 unspecified role unspecified role unspecified role unspecified role Death domain Region 93-184 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false