DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ sheard-2010-1 trini Trichoplusia ni (Cabbage looper) Cabbage looper molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Sheard LB., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds TR., Kobayashi Y., Hsu FF., Sharon M., Browse J., He SY., Rizo J., Howe GA., Zheng N. DIP original experiment: DIP-219272X Nature 2010 IMEx Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Sheard LB., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds TR., Kobayashi Y., Hsu FF., Sharon M., Browse J., He SY., Rizo J., Howe GA., Zheng N. Only protein-protein interactions imex curation sheard-2010-2 trini Trichoplusia ni (Cabbage looper) Cabbage looper ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Sheard LB., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds TR., Kobayashi Y., Hsu FF., Sharon M., Browse J., He SY., Rizo J., Howe GA., Zheng N. DIP original experiment: DIP-219276X Nature 2010 IMEx Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Sheard LB., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds TR., Kobayashi Y., Hsu FF., Sharon M., Browse J., He SY., Rizo J., Howe GA., Zheng N. Only protein-protein interactions imex curation sheard-2010-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sheard LB., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds TR., Kobayashi Y., Hsu FF., Sharon M., Browse J., He SY., Rizo J., Howe GA., Zheng N. DIP original experiment: DIP-219277X Nature 2010 IMEx Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Sheard LB., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds TR., Kobayashi Y., Hsu FF., Sharon M., Browse J., He SY., Rizo J., Howe GA., Zheng N. Only protein-protein interactions imex curation sheard-2010-4 trini Trichoplusia ni (Cabbage looper) Cabbage looper pull down pull down predetermined predetermined participant predetermined Sheard LB., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds TR., Kobayashi Y., Hsu FF., Sharon M., Browse J., He SY., Rizo J., Howe GA., Zheng N. DIP original experiment: DIP-219273X Nature 2010 IMEx Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Sheard LB., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds TR., Kobayashi Y., Hsu FF., Sharon M., Browse J., He SY., Rizo J., Howe GA., Zheng N. Only protein-protein interactions imex curation sheard-2010-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sheard LB., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds TR., Kobayashi Y., Hsu FF., Sharon M., Browse J., He SY., Rizo J., Howe GA., Zheng N. DIP original experiment: DIP-219275X Nature 2010 IMEx Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Sheard LB., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds TR., Kobayashi Y., Hsu FF., Sharon M., Browse J., He SY., Rizo J., Howe GA., Zheng N. Only protein-protein interactions imex curation sheard-2010-6 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Sheard LB., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds TR., Kobayashi Y., Hsu FF., Sharon M., Browse J., He SY., Rizo J., Howe GA., Zheng N. DIP original experiment: DIP-219274X Nature 2010 IMEx Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Sheard LB., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds TR., Kobayashi Y., Hsu FF., Sharon M., Browse J., He SY., Rizo J., Howe GA., Zheng N. Only protein-protein interactions imex curation coi1_arath Coronatine-insensitive protein 1 FBL2 At2g39940 COI1 COI-1 F-box/LRR-repeat protein 2 T28M21.10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEDPDIKRCKLSCVATVDDVIEQVMTYITDPKDRDSASLVCRRWFKIDSETREHVTMALCYTATPDRLSRRFPNLRSLKLKGKPRAAMFNLIPENWGGYVTPWVTEISNNLRQLKSVHFRRMIVSDLDLDRLAKARADDLETLKLDKCSGFTTDGLLSIVTHCRKIKTLLMEESSFSEKDGKWLHELAQHNTSLEVLNFYMTEFAKISPKDLETIARNCRSLVSVKVGDFEILELVGFFKAAANLEEFCGGSLNEDIGMPEKYMNLVFPRKLCRLGLSYMGPNEMPILFPFAAQIRKLDLLYALLETEDHCTLIQKCPNLEVLETRNVIGDRGLEVLAQYCKQLKRLRIERGADEQGMEDEEGLVSQRGLIALAQGCQELEYMAVYVSDITNESLESIGTYLKNLCDFRLVLLDREERITDLPLDNGVRSLLIGCKKLRRFAFYLRQGGLTDLGLSYIGQYSPNVRWMLLGYVGESDEGLMEFSRGCPNLQKLEMRGCCFSERAIAAAVTKLPSLRYLWVQGYRASMTGQDLMQMARPYWNIELIPSRRVPEVNQQGEIREMEHPAHILAYYSLAGQRTDCPTTVRVLKEPI skp1a_arath SKP1-like protein 1A UFO-binding protein 1 SKP1 UIP1 At1g75950 T4O12.17 SKP1A ASK1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSAKKIVLKSSDGESFEVEEAVALESQTIAHMVEDDCVDNGVPLPNVTSKILAKVIEYCKRHVEAAASKAEAVEGAATSDDDLKAWDADFMKIDQATLFELILAANYLNIKNLLDLTCQTVADMIKGKTPEEIRTTFNIKNDFTPEEEEEVRRENQWAFE ti10a_arath Protein TIFY 10A TIFY10A JAZ1 At1g19180 T29M8.5 Jasmonate ZIM domain-containing protein 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSSMECSEFVGSRRFTGKKPSFSQTCSRLSQYLKENGSFGDLSLGMACKPDVNGTLGNSRQPTTTMSLFPCEASNMDSMVQDVKPTNLFPRQPSFSSSSSSLPKEDVLKMTQTTRSVKPESQTAPLTIFYAGQVIVFNDFSAEKAKEVINLASKGTANSLAKNQTDIRSNIATIANQVPHPRKTTTQEPIQSSPTPLTELPIARRASLHRFLEKRKDRVTSKAPYQLCDPAKASSNPQTTGNMSWLGLAAEI coi1-skp1a-1 3215490 N/A 3215496 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3215497 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false coi1-skp1a-2 3215491 N/A 3215496 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3215497 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false coi1-tify10a-3 3215492 N/A 3215498 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3215496 unspecified role unspecified role bait bait O04197:p.Met88Ala M88A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Arg121Ala R121A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Arg85Ala R85A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Phe89Ala F89A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Arg348Ala R348A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Tyr386Ala Y386A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Arg409Ala R409A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Tyr444Ala Y444A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Leu469Ala L469A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Arg496Ala R496A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Leu301Ala L301A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Tyr302Ala Y302A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Arg326Ala R326A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O04197:p.Arg351Ala R351A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false coi1-skp1a-3 3215493 N/A 3215496 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3215497 unspecified role unspecified role prey prey trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false coi1-skp1a-4 3215494 N/A 3215498 unspecified role unspecified role unspecified role unspecified role Region 200-220 Region 200-220 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Region 205-226 Region 205-226 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3215496 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3215497 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false tify10a-coi1-1 3215495 N/A 3215498 unspecified role unspecified role bait bait Region 200-220 Region 200-220 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3215496 unspecified role unspecified role prey prey trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false