DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ tait-2010-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Tait S., Dutta K., Cowburn D., Warwicker J., Doig AJ., McCarthy JE. DIP original experiment: DIP-220038X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Local control of a disorder-order transition in 4E-BP1 underpins regulation of translation via eIF4E. Tait S., Dutta K., Cowburn D., Warwicker J., Doig AJ., McCarthy JE. Only protein-protein interactions imex curation tait-2010-2 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Tait S., Dutta K., Cowburn D., Warwicker J., Doig AJ., McCarthy JE. DIP original experiment: DIP-220039X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Local control of a disorder-order transition in 4E-BP1 underpins regulation of translation via eIF4E. Tait S., Dutta K., Cowburn D., Warwicker J., Doig AJ., McCarthy JE. Only protein-protein interactions imex curation if4e_human Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit EIF4E EIF4EL1 EIF4F protein protein human Homo sapiens Human MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV 4ebp1_human Eukaryotic translation initiation factor 4E-binding protein 1 Phosphorylated heat- and acid-stable protein regulated by insulin 1 EIF4EBP1 protein protein human Homo sapiens Human MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI eif4e-eif4ebp1-15 3215221 N/A 3215223 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3215224 unspecified role unspecified role unspecified role unspecified role eIF4E-binding domain Region 51-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q13541:p.Asp55Ser D55S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13541:p.Lys57Glu K57E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13541:p.[Lys57Glu;Glu61Gly] K57E/E61G mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q13541:p.Met60Thr M60/T/A/E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13541:p.Glu61Lys E61K mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13541:p.Asn64Ser N64S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13541:p.Arg63Gln R63Q mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13541:p.Met60Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q13541:p.Met60Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false eif4e-eif4ebp1-16 3215222 N/A 3215223 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3215224 unspecified role unspecified role unspecified role unspecified role eIF4E-binding domain Region 51-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false