DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ baxter-2010-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight silver stain molecular weight estimation by silver staining weight silver stain Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. DIP original experiment: DIP-220001X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae. Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. Only protein-protein interactions imex curation baxter-2010-2 trini Trichoplusia ni (Cabbage looper) Cabbage looper anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. DIP original experiment: DIP-220008X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae. Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. Only protein-protein interactions imex curation baxter-2010-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight silver stain molecular weight estimation by silver staining weight silver stain Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. DIP original experiment: DIP-220006X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae. Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. Only protein-protein interactions imex curation baxter-2010-4 trini Trichoplusia ni (Cabbage looper) Cabbage looper anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. DIP original experiment: DIP-220009X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae. Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. Only protein-protein interactions imex curation baxter-2010-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. DIP original experiment: DIP-220004X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae. Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. Only protein-protein interactions imex curation baxter-2010-6 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. DIP original experiment: DIP-220002X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae. Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. Only protein-protein interactions imex curation baxter-2010-7 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. DIP original experiment: DIP-220005X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae. Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. Only protein-protein interactions imex curation baxter-2010-8 in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. DIP original experiment: DIP-220003X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae. Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. Only protein-protein interactions imex curation baxter-2010-9 trini Trichoplusia ni (Cabbage looper) Cabbage looper anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. DIP original experiment: DIP-220007X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae. Baxter RH., Steinert S., Chelliah Y., Volohonsky G., Levashina EA., Deisenhofer J. Only protein-protein interactions imex curation a7urs6_anoga 5666907 AgaP_AGAP007033 APL1C protein protein african malaria mosquito Anopheles gambiae African malaria mosquito MCWLHAVSLISVIFFTCGSVAAVGGNNYWYTEEQPVATTKPSFHVNGYNIYGSQPANGGLPANGGPTYRSETNFGGQPANGGLPANGGLPANGGLPANGGLLANGGLPANGGLPAKRGQLPYGTPTHIGQTIYRSQPNYGGQQRYNARPRQLEYKCIEGNLQYDCVFYDVHIDMQTQDVYFGFEDITLNNQKIVTFKNSTMRKLPAALLASFRQVEVLNLNGLQIEQIDTNAFAYAHTIQKLYMGFNAIRYLPPYVFQNVPLLTVLVLERNDLTSLPRGIFHNTPKLSMLSMSNNNLERIEDDTFQATTALQNLQLSSNRLTHVDLSLIPSLFHVNVSYNLLSTLAIPIAVEELDASHNSINAVRGPVNVELTILKLQHNNLTDTAWLLNYPGLVDVDLSYNQLEKITYQHFVKMQRLERLYVSNNHLVALNLYSQPIPTLKVLDLSHNHLLHVERNQPQFDRVEYLYLDHNSIVTLKLSTSHTLKNLTLSHNDWDCNSLRALFRNVAHPAVHDADQHCKIDYQLEQGLCCKESDKPYLDRLLQYIALTSVVEKLQRAQGRCSATDTINSVQSLSHYITQQGDVPLQGNEQLEAEVNELRAEVQQLTNEQIQQEQLLQGLHAEIDTNLRRYRLPKDGLARSSDNLNKVFTHLKERHAFKLRETQARRTEANAKQKETEDLEQENIALEKQLDNKKAKQAELRQETSQKRQKVKQLEAKKNRNPDTRRVTK q7q5n3_anoga 1276956 AgaP_AGAP006348 protein protein african malaria mosquito Anopheles gambiae African malaria mosquito MMSLQACQVVLLLVCVTATVHGAIHEIKQNGNRYKIEKVTDSSLKQALASLRQSAWNVKELDLSGNPLSQISAADLAPFTKLELLNLSSNVLYETLDLESLSTLRTLDLNNNYVQELLVGPSIETLHAANNNISRVSCSRGQGKKNIYLANNKITMLRDLDEGCRSRVQYLDLKLNEIDTVNFAELAASSDTLEHLNLQYNFIYDVKGQVVFAKLKTLDLSSNKLAFMGPEFQSAAGVTWISLRNNKLVLIEKALRFSQNLEHFDLRGNGFHCGTLRDFFSKNQRVQTVAKQTVKKLTGQNEEECTVPTLGHYGAYCCEDLPAPFADRLIALKRKEHALLSGQGSETERLECERENQARQREIDALKEQYRTVIDQVTLRKQAKITLEQKKKALDEQVSNGRRAHAELDGTLQQAVGQIELQHATEEQSPLQLLRAIVKRYEEMYVEQQSVQNNAIRDWDMYQHKETQLAEENARLKKLNGEADLALASANATLQELVVREQNLASQLG q9gyw4_anoga TEP-I protein protein african malaria mosquito Anopheles gambiae African malaria mosquito MWQFIRSRILTVIIFIGAAHGLLVVGPKFIRANQEYTLVISNFNSQLSKVDLLLKLEGETDNGLSVLNVTKMVDVRRNMNRMINFNMPEELTAGNYKITIDGQRGFSFHKEAELVYLSKSISGLIQVDKPVFKPGDTVNFRVILLDTELKPPARVKSVYVTIRDPQRNVIRKWSTAKLYAGVFESDLQIVPTPMLGVWNISVEVEGEELVSKTFEVKEYVLSTFDVQVMPSVIPLEEHQAVNLTIEANYHFGKPVQGVAKVELYLDDDKLNQKKELTVYGKGQVELRFDNFAMDADQQDVRVKVSFIEQYTNRTVVKQSQITVYRYAYRVELIKESPQFRPGLPFKCALQFTHHDGTPAKGITGKVEVSDVGFETTTTSDNDGLIKLELQPSEGTEQLGINFNAVDGFFFYEDVNKVETVTDAYIKLELKSPIKRNKLMRFMVTCTERMTFFVYYVMSKGNIIDAGFMRPNKQTKYLLQLNATEKMIPKAKILIATVAGRTVVYDYADLDFQELRNNFDLSIDEQEIKPGRQIELSMSGRPGAYVGLAAYDKALLLFNKNHDLFWEDIGQVFDGFHAINENEFDIFHSLGLFARTLDDILFDSANEKTGRNALQSGKPIGKLVSYRTNFQESWLWKNVSIGRSGSRKLIEVVPDTTTSWYLTGFSIDPVYGLGIIKKPIQFTTVQPFYIVENLPYSIKRGEAVVLQFTLFNNLGAEYIADVTLYNVANQTEFVGRPDTDLSYTKSVSVPPKVGVPISFLIKARKLGEMAVRVKASIMLGHETDALEKVIRVMPESLAQPKMDTSFFCFDDYKNQTFPFNLDINKKADNGSKKIEFRLNPNLLTMVIKNLDNLLAVPTGCGEQNMVKFVPNILVLDYLYATGSKEQHLIDKATNLLRQGYQNQMRYRQTDGSFGVWEKSGSSVFLTAFVATSMQTASKYMNDIDAAMVEKALDWLASKQHSSGRFDETGKVWHKDMQGGLRNGVALTSYVLTALLENDIAKVKHAVVIQNGMNYLSNQLAFINNPYDLSIATYAMMLNGHTMKKEALDKLIDMSISDNNKKERYWGTTNQIETTAYALLSFVMAEKYLDGIPVMNWLVNQRYVTGSFPRTQDTFVGLKALTKLAEKISPSRNDYTVQLKYKKNTKYFNINSEQIDVQNFLEIPEDTKKLEINVGGIGFGLLEVIYQFDLNLVNFEHRFKLDLEKQNTGSDYELRLRVCANYIPELTDSQSNMALIEVTLPSGYVVDRNPISEQTTVNPIQNMEIRYGGTSVVLYYYKMGTERNCFTVTAYRRFKVALKRPAYVVVYDYYNTNLNAIKVYEVDKQNVCEICEEEDCPAECKK apl1c-q7q5n3_anoga-1 3214277 N/A 3214286 unspecified role unspecified role unspecified role unspecified role Region 131-730 Region 131-730 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3214287 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false q7q5n3_anoga-apl1c-1 3214278 N/A 3214288 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3214286 unspecified role unspecified role prey prey Region 131-730 Region 131-730 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3214287 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false trini Trichoplusia ni (Cabbage looper) Cabbage looper association association false false false apl1c-q7q5n3_anoga-2 3214279 N/A 3214288 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3214286 unspecified role unspecified role unspecified role unspecified role Region 131-730 Region 131-730 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3214287 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false tep_i-apl1c-1 3214280 N/A 3214288 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3214286 unspecified role unspecified role prey prey Region 131-730 Region 131-730 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3214287 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false trini Trichoplusia ni (Cabbage looper) Cabbage looper association association false false false apl1c-q7q5n3_anoga-3 3214281 N/A 3214286 unspecified role unspecified role unspecified role unspecified role Region 131-730 Region 131-730 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3214287 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false apl1c-q7q5n3_anoga-4 3214282 N/A 3214286 unspecified role unspecified role unspecified role unspecified role Region 131-730 Region 131-730 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3214287 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false apl1c-q7q5n3_anoga-5 3214283 N/A 3214286 unspecified role unspecified role unspecified role unspecified role Region 131-730 Region 131-730 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3214287 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper covalent binding covalent binding false false false apl1c-q7q5n3_anoga-6 3214284 N/A 3214286 unspecified role unspecified role unspecified role unspecified role Region 131-730 Region 131-730 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3214287 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false q7q5n3_anoga-apl1c-2 3214285 N/A 3214286 unspecified role unspecified role prey prey Region 131-730 Region 131-730 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3214287 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false