DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ takeshita-2010-1 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Takeshita D., Tomita K. DIP original experiment: DIP-219959X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Takeshita D., Tomita K. Only protein-protein interactions imex curation takeshita-2010-2 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Takeshita D., Tomita K. DIP original experiment: DIP-219963X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Takeshita D., Tomita K. Only protein-protein interactions imex curation takeshita-2010-3 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Takeshita D., Tomita K. DIP original experiment: DIP-219961X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Takeshita D., Tomita K. Only protein-protein interactions imex curation takeshita-2010-4 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Takeshita D., Tomita K. DIP original experiment: DIP-219964X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Takeshita D., Tomita K. Only protein-protein interactions imex curation takeshita-2010-5 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Takeshita D., Tomita K. DIP original experiment: DIP-219960X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Takeshita D., Tomita K. Only protein-protein interactions imex curation takeshita-2010-6 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Takeshita D., Tomita K. DIP original experiment: DIP-219962X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Takeshita D., Tomita K. Only protein-protein interactions imex curation eftu1_ecoli Elongation factor Tu 1 tufA b3339 P-43 JW3301 Bacteriophage Q beta RNA-directed RNA polymerase subunit III protein protein ecoli Escherichia coli (strain K12) MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAARAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG efts_ecoli Elongation factor Ts tsf Bacteriophage Q beta RNA-directed RNA polymerase subunit IV JW0165 b0170 protein protein ecoli Escherichia coli (strain K12) MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKSGAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVAGKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKGADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEGRMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEVAAMSKQS rdrp_bpqbe RNA-directed RNA polymerase subunit beta RNA replicase beta chain RNA-directed RNA polymerase subunit II protein protein bacteriophage q-beta Enterobacteria phage Qbeta MSKTASSRNSLSAQLRRAANTRIEVEGNLALSIANDLLLAYGQSPFNSEAECISFSPRFDGTPDDFRINYLKAEIMSKYDDFSLGIDTEAVAWEKFLAAEAECALTNARLYRPDYSEDFNFSLGESCIHMARRKIAKLIGDVPSVEGMLRHCRFSGGATTTNNRSYGHPSFKFALPQACTPRALKYVLALRASTHFDIRISDISPFNKAVTVPKNSKTDRCIAIEPGWNMFFQLGIGGILRDRLRCWGIDLNDQTINQRRAHEGSVTNNLATVDLSAASDSISLALCELLLPPGWFEVLMDLRSPKGRLPDGSVVTYEKISSMGNGYTFELESLIFASLARSVCEILDLDSSEVTVYGDDIILPSCAVPALREVFKYVGFTTNTKKTFSEGPFRESCGKHYYSGVDVTPFYIRHRIVSPADLILVLNNLYRWATIDGVWDPRAHSVYLKYRKLLPKQLQRNTIPDGYGDGALVGSVLINPFAKNRGWIRYVPVITDHTRDRERAELGSYLYDLFSRCLSESNDGLPLRGPSGCDSADLFAIDQLICRSNPTKISRSTGKFDIQYIACSSRVLAPYGVFQGTKVASLHEA This sequence has been withdrawn from Uniprot. 8C02DB50F7202DEF eftu-qbeta-1 3213366 N/A 3213372 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3213373 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3213374 unspecified role unspecified role prey prey ecolx Escherichia coli association association false false false tsf-eftu-1 3213367 N/A 3213372 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3213373 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false eftu-qbeta-2 3213368 N/A 3213372 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P0CE47:p.Phe262Ala F262A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0CE47:p.Phe262_Arg263del Δ262-263 mutation disrupting mutation disrupting interaction mutation disrupting deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3213374 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association false false false tsf-qbeta-1 3213369 N/A 3213373 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region Δ187-226 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3213374 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association false false false tsf-eftu-2 3213370 N/A 3213372 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3213373 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3213374 unspecified role unspecified role prey prey ecolx Escherichia coli association association false false false eftu-tsf-1 3213371 N/A 3213372 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3213373 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false