DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ elden-2010-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. DIP original experiment: DIP-219402X Nature 2010 IMEx Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. Only protein-protein interactions imex curation elden-2010-2 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. DIP original experiment: DIP-219404X Nature 2010 IMEx Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. Only protein-protein interactions imex curation elden-2010-3 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. DIP original experiment: DIP-219403X Nature 2010 IMEx Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. Only protein-protein interactions imex curation elden-2010-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. DIP original experiment: DIP-219405X Nature 2010 IMEx Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. Only protein-protein interactions imex curation pbp1_yeast PAB1-binding protein 1 PBP1 MRS16 Poly(A)-binding protein-binding protein YGR178C protein protein yeast Saccharomyces cerevisiae Baker's yeast MKGNFRKRDSSTNSRKGGNSDSNYTNGGVPNQNNSSMFYENPEITRNFDDRQDYLLANSIGSDVTVTVTSGVKYTGLLVSCNLESTNGIDVVLRFPRVADSGVSDSVDDLAKTLGETLLIHGEDVAELELKNIDLSLDEKWENSKAQETTPARTNIEKERVNGESNEVTKFRTDVDISGSGREIKERKLEKWTPEEGAEHFDINKGKALEDDSASWDQFAVNEKKFGVKSTFDEHLYTTKINKDDPNYSKRLQEAERIAKEIESQGTSGNIHIAEDRGIIIDDSGLDEEDLYSGVDRRGDELLAALKSNSKPNSNKGNRYVPPTLRQQPHHMDPAIISSSNSNKNENAVSTDTSTPAAAGAPEGKPPQKTSKNKKSLSSKEAQIEELKKFSEKFKVPYDIPKDMLEVLKRSSSTLKSNSSLPPKPISKTPSAKTVSPTTQISAGKSESRRSGSNISQGQSSTGHTTRSSTSLRRRNHGSFFGAKNPHTNDAKRVLFGKSFNMFIKSKEAHDEKKKGDDASENMEPFFIEKPYFTAPTWLNTIEESYKTFFPDEDTAIQEAQTRFQQRQLNSMGNAVPGMNPAMGMNMGGMMGFPMGGPSASPNPMMNGFAAGSMGMYMPFQPQPMFYHPSMPQMMPVMGSNGAEEGGGNISPHVPAGFMAAGPGAPMGAFGYPGGIPFQGMMGSGPSGMPANGSAMHSHGHSRNYHQTSHHGHHNSSTSGHK 92005F3A2346193E tadbp_human TAR DNA-binding protein 43 TARDBP TDP43 protein protein human Homo sapiens Human MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM atx2_human Ataxin-2 Spinocerebellar ataxia type 2 protein Trinucleotide repeat-containing gene 13 protein SCA2 TNRC13 ATXN2 ATX2 protein protein human Homo sapiens Human MRSAAAAPRSPAVATESRRFAAARWPGWRSLQRPARRSGRGGGGAAPGPYPSAAPPPPGPGPPPSRQSSPPSASDCFGSNGNGGGAFRPGSRRLLGLGGPPRPFVVLLLPLASPGAPPAAPTRASPLGARASPPRSGVSLARPAPGCPRPACEPVYGPLTMSLKPQQQQQQQQQQQQQQQQQQQQQQQPPPAAANVRKPGGSGLLASPAAAPSPSSSSVSSSSATAPSSVVAATSGGGRPGLGRGRNSNKGLPQSTISFDGIYANMRMVHILTSVVGSKCEVQVKNGGIYEGVFKTYSPKCDLVLDAAHEKSTESSSGPKREEIMESILFKCSDFVVVQFKDMDSSYAKRDAFTDSAISAKVNGEHKEKDLEPWDAGELTANEELEALENDVSNGWDPNDMFRYNEENYGVVSTYDSSLSSYTVPLERDNSEEFLKREARANQLAEEIESSAQYKARVALENDDRSEEEKYTAVQRNSSEREGHSINTRENKYIPPGQRNREVISWGSGRQNSPRMGQPGSGSMPSRSTSHTSDFNPNSGSDQRVVNGGVPWPSPCPSPSSRPPSRYQSGPNSLPPRAATPTRPPSRPPSRPSRPPSHPSAHGSPAPVSTMPKRMSSEGPPRMSPKAQRHPRNHRVSAGRGSISSGLEFVSHNPPSEAATPPVARTSPSGGTWSSVVSGVPRLSPKTHRPRSPRQNSIGNTPSGPVLASPQAGIIPTEAVAMPIPAASPTPASPASNRAVTPSSEAKDSRLQDQRQNSPAGNKENIKPNETSPSFSKAENKGISPVVSEHRKQIDDLKKFKNDFRLQPSSTSESMDQLLNKNREGEKSRDLIKDKIEPSAKDSFIENSSSNCTSGSSKPNSPSISPSILSNTEHKRGPEVTSQGVQTSSPACKQEKDDKEEKKDAAEQVRKSTLNPNAKEFNPRSFSQPKPSTTPTSPRPQAQPSPSMVGHQQPTPVYTQPVCFAPNMMYPVPVSPGVQPLYPIPMTPMPVNQAKTYRAVPNMPQQRQDQHHQSAMMHPASAAGPPIAATPPAYSTQYVAYSPQQFPNQPLVQHVPHYQSQHPHVYSPVIQGNARMMAPPTHAQPGLVSSSATQYGAHEQTHAMYACPKLPYNKETSPSFYFAISTGSLAQQYAHPNATLHPHTPHPQPSATPTGQQQSQHGGSHPAPSPVQHHQHQAAQALHLASPQQQSAIYHAGLAPTPPSMTPASNTQSPQNSFPAAQQTVFTIHPSHVQPAYTNPPHMAHVPQAHVQSGMVPSHPTAHAPMMLMTTQPPGGPQAALAQSALQPIPVSTTAHFPYMTHPSVQAHHQQQL pbp1-tardbp-1 3213218 N/A 3213222 unspecified role unspecified role bait bait CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3213223 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false atxn2-tardbp-1 3213219 N/A 3213224 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3213223 unspecified role unspecified role prey prey YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false tardbp-atxn2-1 3213220 N/A 3213224 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3213223 unspecified role unspecified role bait bait YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q13148:p.Phe229Leu F229L mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13148:p.[Phe147Leu;Phe149Leu] F147L/F149L mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q13148:p.[Phe194Leu;Phe229Leu;Phe231Leu] F194L/F229L/F231L mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q13148:p.[Phe147Leu;Phe149Leu;Phe194Leu;Phe229Leu;Phe231Leu] F147L/F149L/F194L/F229L/F231L mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false tardbp-atxn2-2 3213221 N/A 3213224 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3213223 unspecified role unspecified role bait bait YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false