DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ducka-2010-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ducka AM., Joel P., Popowicz GM., Trybus KM., Schleicher M., Noegel AA., Huber R., Holak TA., Sitar T. DIP original experiment: DIP-221265X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleation. Ducka AM., Joel P., Popowicz GM., Trybus KM., Schleicher M., Noegel AA., Huber R., Holak TA., Sitar T. Only protein-protein interactions imex curation ducka-2010-2 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Ducka AM., Joel P., Popowicz GM., Trybus KM., Schleicher M., Noegel AA., Huber R., Holak TA., Sitar T. DIP original experiment: DIP-221268X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleation. Ducka AM., Joel P., Popowicz GM., Trybus KM., Schleicher M., Noegel AA., Huber R., Holak TA., Sitar T. Only protein-protein interactions imex curation ducka-2010-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ducka AM., Joel P., Popowicz GM., Trybus KM., Schleicher M., Noegel AA., Huber R., Holak TA., Sitar T. DIP original experiment: DIP-221266X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleation. Ducka AM., Joel P., Popowicz GM., Trybus KM., Schleicher M., Noegel AA., Huber R., Holak TA., Sitar T. Only protein-protein interactions imex curation ducka-2010-4 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Ducka AM., Joel P., Popowicz GM., Trybus KM., Schleicher M., Noegel AA., Huber R., Holak TA., Sitar T. DIP original experiment: DIP-221269X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleation. Ducka AM., Joel P., Popowicz GM., Trybus KM., Schleicher M., Noegel AA., Huber R., Holak TA., Sitar T. Only protein-protein interactions imex curation ducka-2010-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ducka AM., Joel P., Popowicz GM., Trybus KM., Schleicher M., Noegel AA., Huber R., Holak TA., Sitar T. DIP original experiment: DIP-221267X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleation. Ducka AM., Joel P., Popowicz GM., Trybus KM., Schleicher M., Noegel AA., Huber R., Holak TA., Sitar T. Only protein-protein interactions imex curation q9u1k1-1 Protein spire p150-Spir CG10076 spir protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAMSSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYHQVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIVDQPDHSASSSGDSSVINRAFDNSNHHHHHQHHHPPLVVSHRKIISELAEIVYTALDYNLPEDEECQVSQELENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIETCRNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNEAGAEKLIKASESSATTQQELAKLGFNDWARFWVQVIDELRRGVRLKKSNHERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKASDRQLGPPRMCEPSPREQLMESIRKGKELKQITPPEAPTLRERVLPSANSTLSRSRQRLIKVDFSKFQDDDLFYDENSISSSHSTAATHQHHPHFAEMHRCSQPKMPPYPFGGYMVPSQARQDCRETASLMRPRRTMEPAKQVPPPEEPSFTKDEYHKFYDTALESYDLATQCESRRASLRRHTIVGCQSNLDETHSMPPTRPESRQSDDVSKETPKRSPAEQTHPSDEGSSTSSLGPWNKSFMDKQTWMERGDDRLSVTLAEIVHIRSVMTKAELEGLPMDVRVKEDVEKRRVCFLCLRTRFSFFGPWGIQCKLCQRTVCAKCYTKMRIPSEHFRNVPLVLISPSLLSSPASSSTPSPSHHAQQAHSSSTGNIMDDQFPKSLIERLLRSESDRKTRSTVGSAPSSPKHQRSNMSTPGISVGPGASSSSAAATGQAVEALHDQATMSSSYSAAMRPSGVHQQQKQHYNNAMSRSMEGPRSLPVHSPAYRPLSNNSTLERKSRFSRGFNLFSSGSHLAQTQEQKENLRGEQVTVCNDCQGLVNEITSSVKQKRSSARNRTIQNLTLDLTPVWK act1_drome Actin-5C Act5C CG4027 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MCDEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF act5c-spir-1 3210138 N/A 3210143 unspecified role unspecified role unspecified role unspecified role Region 432-480 Region 432-480 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3210144 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false act5c-spir-2 3210139 N/A 3210143 unspecified role unspecified role unspecified role unspecified role Region 432-480 Region 432-480 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3210144 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false act5c-spir-3 3210140 N/A 3210143 unspecified role unspecified role unspecified role unspecified role Region 432-480 Region 432-480 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3210144 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false acta1-spir-1 3210141 N/A 3210145 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3210143 unspecified role unspecified role unspecified role unspecified role Region 396-480 Region 396-480 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false acta1-spir-2 3210142 N/A 3210145 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3210143 unspecified role unspecified role unspecified role unspecified role Region 461-480 Region 461-480 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false