DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hsia-2010-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hsia KC., Hoelz A. DIP original experiment: DIP-219827X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Crystal structure of alpha-COP in complex with epsilon-COP provides insight into the architecture of the COPI vesicular coat. Hsia KC., Hoelz A. Only protein-protein interactions imex curation hsia-2010-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hsia KC., Hoelz A. DIP original experiment: DIP-219829X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Crystal structure of alpha-COP in complex with epsilon-COP provides insight into the architecture of the COPI vesicular coat. Hsia KC., Hoelz A. Only protein-protein interactions imex curation hsia-2010-3 ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Hsia KC., Hoelz A. DIP original experiment: DIP-219830X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Crystal structure of alpha-COP in complex with epsilon-COP provides insight into the architecture of the COPI vesicular coat. Hsia KC., Hoelz A. Only protein-protein interactions imex curation hsia-2010-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hsia KC., Hoelz A. DIP original experiment: DIP-219832X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Crystal structure of alpha-COP in complex with epsilon-COP provides insight into the architecture of the COPI vesicular coat. Hsia KC., Hoelz A. Only protein-protein interactions imex curation hsia-2010-5 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Hsia KC., Hoelz A. DIP original experiment: DIP-219828X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Crystal structure of alpha-COP in complex with epsilon-COP provides insight into the architecture of the COPI vesicular coat. Hsia KC., Hoelz A. Only protein-protein interactions imex curation hsia-2010-6 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Hsia KC., Hoelz A. DIP original experiment: DIP-219831X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Crystal structure of alpha-COP in complex with epsilon-COP provides insight into the architecture of the COPI vesicular coat. Hsia KC., Hoelz A. Only protein-protein interactions imex curation cope_yeast Coatomer subunit epsilon SEC28 YIL076W Epsilon-coat protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MDYFNIKQNYYTGNFVQCLQEIEKFSKVTDNTLLFYKAKTLLALGQYQSQDPTSKLGKVLDLYVQFLDTKNIEELENLLKDKQNSPYELYLLATAQAILGDLDKSLETCVEGIDNDEAEGTTELLLLAIEVALLNNNVSTASTIFDNYTNAIEDTVSGDNEMILNLAESYIKFATNKETATSNFYYYEELSQTFPTWKTQLGLLNLHLQQRNIAEAQGIVELLLSDYYSVEQKENAVLYKPTFLANQITLALMQGLDTEDLTNQLVKLDHEHAFIKHHQEIDAKFDELVRKYDTSN 95ACA8BB647817DB copa_yeast Coatomer subunit alpha COP1 RET1 SEC33 YDL145C D1578 SOO1 Alpha-coat protein Retrieval from endoplasmic reticulum protein 1 Secretory protein 22 Suppressor of osmo-sensitivity 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKMLTKFESKSTRAKGIAFHPSRPWVLVALFSSTIQLWDYRMGTLLHRFEDHEGPVRGLDFHPTQPIFVSAGDDYTIKVWSLDTNKCLYTLTGHLDYVRTVFFHRELPWIISASDDQTIRIWNWQNRKEIACLTGHNHFVMCAQFHPTDDLIVSASLDETIRIWDISGLRKRHSAPGTSSFEEQMSAQQNLLDGSLGDCVVKFILEGHTRGVNWASFHPTLPLIVSGSDDRQVKLWRMSATKAWEVDTCRGHTNNVDSVIFHPHQNLIISVGEDKTLRVWDLDKRTPVKQFKRENDRFWLIAAHPHINLFGAAHDSGIMVFKLDRERPCSFIHQNQLFFVNAEKQIQSFNFQKRVASLPYASLKGIGQPWDAFRSISYNPSQHSVLVNEANGKFALVILPKQPVGAVEPTSVTQDTGNFATFVGRNRFVVYNKNTESVEVRSLENKVTRNIKVEETVRTIVAAGPGSVLVIHPREVILYDVQQGKKVSQLAVKNVKYVSWSLDGQYVALMSKHTITLATKKLELINSMHETIRIKSAAWDETGVLIYSTLNHIRYSLLNGDRGIIKTLEKTLYITKVQGKLVYCLNREGEIEILTIDPTEYRFKKALVNKNFPEVLRLIKDSNLVGQNIISYLQKSGYPEIALQFVQDPHIRFDLALEYGNLDVALDEAKKLNDSSTWERLIQEALAQGNASLAEMIYQTQHSFDKLSFLYLVTGDVNKLSKMQNIAQTREDFGSMLLNTFYNNSTKERSSIFAEGGSLPLAYAVAKANGDEAAASAFLEQAEVDEQDVTLPDQMDASNFVQRPVISKPLEKWPLKEAELSYFEKAVLGQIDDLTIDDETPAVNTTQEQEEPLGEENFNDEDIGEDEGAWDLGDEDLDVGEELPEEVEQGEITSPAQEVETAIWIKNSKLPAVLVAAGAFDAAVQALSKQVGVVKLEPLKKYFTNIYEGCRTYIPSTPCELPAQLGYVRAYDDTVSEDQILPYVPGLDVVNEKMNEGYKNFKLNKPDIAIECFREAIYRITLLMVDDAEDEKLAHKILETAREYILGLSIELERRSLKEGNTVRMLELAAYFTKAKLSPIHRTNALQVAMSQHFKHKNFLQASYFAGEFLKIISSGPRAEQARKIKNKADSMASDAIPIDFDPYAKFDICAATYKPIYEDTPSVSDPLTGSKYVITEKDKIDRIAMISKIGAPASGLRIRV D3589073106F5570 dsl1_yeast Protein transport protein DSL1 Dependent on SLY1-20 protein 1 YNL258C N0842 DSL1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MESLFPNKGEIIRELLKDPLILKNDSKRSNGSELELDSSDLLQREAILANELNILDNLKTFLNLIKEVKTNLNILELENCYYSLQSLRKKMRNNAAYLKQSFNFQQSISTYVDTLHLELVSTLYKILTNGFWKITENSIQFTPTVEWGKDKVHIEYDTFMDFVAQQYFPKGSLDNQAWFILDMTSADSQEQVRAKLNTIMKEYMNLSRIVSMIKNSIFISGKEISYENEKNILVFSKSSSHGQHCVSTVLTSFEAVCDFMLDGLAFRDRKTLSYELGPLFNTEFTKFVKNNASIILESLDSPLKNLVSVINNKLTRLVAKSEVTNWTHSGKEIQDLLMNKQLYYNLLLDKVLESHISEIRSIFEDPKKSWQNLEVVELTTSNTNTMSEKIGKNDSDVQNEKELHNAVSKDDDWNWEVEDDDADAWGDEIDVNIDDEEEKTNQEKEKEPEEEENAWDEAWAIDENIDDASLENGKEHLKAHDVGSLDKDHIEVTQLPKLFLAISQNFKSSFADSHVDEQYFAYKYNLLQTSYMAMCTANFSHNWCQLYVDMRYLIERDEKLYRIKELTRNLLETKLNMKYRIVCQLIRHQLTEFRENERNPSWDATIEKLLPYILKEIVRPLQKIRGEEGSRYLLSFLNFLYNDCVTKEILKWQIISEVNSENLGELVSLLVNNTDIQLLAKEPSYKKMREKFATMGKFLPLHLKEIMEMFYNGDFYLFATDELIQWIELLFADTPLRRNAIDDIYEIRGTALDD 51EDB17F2C55A917 alpha_cop-epsilon_cop-1 3209592 N/A 3209598 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3209599 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 900-1201 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false alpha_cop-epsilon_cop-2 3209593 N/A 3209598 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3209599 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 900-1201 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false alpha_cop-epsilon_cop-3 3209594 N/A 3209598 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3209599 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 900-1201 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false alpha_cop-dsl1-1 3209595 N/A 3209600 unspecified role unspecified role unspecified role unspecified role Region 410-440 Region 410-440 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3209598 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3209599 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 900-1201 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false alpha_cop-epsilon_cop-4 3209596 N/A 3209598 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3209599 unspecified role unspecified role bait bait C-terminal domain Region 900-1201 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false alpha_cop-epsilon_cop-5 3209597 N/A 3209598 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3209599 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 900-1201 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false