DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zheng-2010-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zheng J., Jia Z. DIP original experiment: DIP-219482X Nature 2010 IMEx Structure of the bifunctional isocitrate dehydrogenase kinase/phosphatase. Zheng J., Jia Z. Only protein-protein interactions imex curation zheng-2010-5 in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Zheng J., Jia Z. DIP original experiment: DIP-219485X Nature 2010 IMEx Structure of the bifunctional isocitrate dehydrogenase kinase/phosphatase. Zheng J., Jia Z. Only protein-protein interactions imex curation zheng-2010-6 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zheng J., Jia Z. DIP original experiment: DIP-219483X Nature 2010 IMEx Structure of the bifunctional isocitrate dehydrogenase kinase/phosphatase. Zheng J., Jia Z. Only protein-protein interactions imex curation zheng-2010-7 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Zheng J., Jia Z. DIP original experiment: DIP-219484X Nature 2010 IMEx Structure of the bifunctional isocitrate dehydrogenase kinase/phosphatase. Zheng J., Jia Z. Only protein-protein interactions imex curation idh_ecoli Isocitrate dehydrogenase [NADP] icd icdA icdE b1136 JW1122 Oxalosuccinate decarboxylase NADP(+)-specific ICDH IDP protein protein ecoli Escherichia coli (strain K12) MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPAMLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGPLTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIYAGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAIANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIVIKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECALFEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYDFERLMDGAKLLKCSEFGDAIIENM acek_ecoli Isocitrate dehydrogenase kinase/phosphatase aceK b4016 JW3976 protein protein ecoli Escherichia coli (strain K12) MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDLPLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRRQRFSVRYGEMLF acek-icd-1 3209396 N/A 3209400 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3209401 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false acek-icd-2 3209397 N/A 3209400 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3209401 enzyme enzyme unspecified role unspecified role P11071:p.Asp477Ala D477A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P11071:p.Lys346Glu K346E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation false false false acek-icd-3 3209398 N/A 3209400 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3209401 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false acek-icd-4 3209399 N/A 3209400 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3209401 enzyme enzyme unspecified role unspecified role P11071:p.Lys336Ala K336A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P11071:p.Asp475Ala D475A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P11071:p.Asn462Ala N462A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P11071:p.[Glu497Lys;Glu499Lys] E497K/E499K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false