DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ plant-2010-1 crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Plant LD., Dementieva IS., Kollewe A., Olikara S., Marks JD., Goldstein SA. DIP original experiment: DIP-221068X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx One SUMO is sufficient to silence the dimeric potassium channel K2P1. Plant LD., Dementieva IS., Kollewe A., Olikara S., Marks JD., Goldstein SA. Only protein-protein interactions imex curation plant-2010-2 crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Plant LD., Dementieva IS., Kollewe A., Olikara S., Marks JD., Goldstein SA. DIP original experiment: DIP-221070X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx One SUMO is sufficient to silence the dimeric potassium channel K2P1. Plant LD., Dementieva IS., Kollewe A., Olikara S., Marks JD., Goldstein SA. Only protein-protein interactions imex curation plant-2010-3 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Plant LD., Dementieva IS., Kollewe A., Olikara S., Marks JD., Goldstein SA. DIP original experiment: DIP-221072X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx One SUMO is sufficient to silence the dimeric potassium channel K2P1. Plant LD., Dementieva IS., Kollewe A., Olikara S., Marks JD., Goldstein SA. Only protein-protein interactions imex curation plant-2010-4 crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Plant LD., Dementieva IS., Kollewe A., Olikara S., Marks JD., Goldstein SA. DIP original experiment: DIP-221069X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx One SUMO is sufficient to silence the dimeric potassium channel K2P1. Plant LD., Dementieva IS., Kollewe A., Olikara S., Marks JD., Goldstein SA. Only protein-protein interactions imex curation plant-2010-5 crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Plant LD., Dementieva IS., Kollewe A., Olikara S., Marks JD., Goldstein SA. DIP original experiment: DIP-221071X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx One SUMO is sufficient to silence the dimeric potassium channel K2P1. Plant LD., Dementieva IS., Kollewe A., Olikara S., Marks JD., Goldstein SA. Only protein-protein interactions imex curation sumo1_human Small ubiquitin-related modifier 1 SUMO1 Sentrin Ubiquitin-like protein SMT3C Ubiquitin-homology domain protein PIC1 Ubiquitin-like protein UBL1 GAP-modifying protein 1 SMT3 homolog 3 SMT3C SMT3H3 UBL1 OK/SW-cl.43 protein protein human Homo sapiens Human MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV kcnk1_human Potassium channel subfamily K member 1 KCNK1 HOHO1 KCNO1 TWIK1 Inward rectifying potassium channel protein TWIK-1 Potassium channel KCNO1 Potassium channel K2P1 protein protein human Homo sapiens Human MLQSLAGSSCVRLVERHRSAWCFGFLVLGYLLYLVFGAVVFSSVELPYEDLLRQELRKLKRRFLEEHECLSEQQLEQFLGRVLEASNYGVSVLSNASGNWNWDFTSALFFASTVLSTTGYGHTVPLSDGGKAFCIIYSVIGIPFTLLFLTAVVQRITVHVTRRPVLYFHIRWGFSKQVVAIVHAVLLGFVTVSCFFFIPAAVFSVLEDDWNFLESFYFCFISLSTIGLGDYVPGEGYNQKFRELYKIGITCYLLLGLIAMLVVLETFCELHELKKFRKMFYVKKDKDEDQVHIIEHDQLSFSSITDQAAGMKEDQKQNEPFVATQSSACVDGPANH ubc9_human SUMO-conjugating enzyme UBC9 UBE2I p18 SUMO-protein ligase UBC9 UBCE9 Ubiquitin carrier protein 9 Ubiquitin carrier protein I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I RING-type E3 SUMO transferase UBC9 protein protein human Homo sapiens Human MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS kcnk1-sumo1-1 3209202 N/A 3209207 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster N/A 3209208 unspecified role unspecified role fluorescence donor fluorescence donor O00180:p.Lys274Gln K274Q/R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O00180:p.Lys274Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster proximity proximity close-contact colocalization neighbourhood interaction false false false kcnk1-5 3209203 N/A 3209208 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster N/A 3209208 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster proximity proximity close-contact colocalization neighbourhood interaction false false false kcnk1-sumo1-2 3209204 N/A 3209207 unspecified role unspecified role prey prey Region 1-97 Region 1-97 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3209208 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 270-336 Region 270-336 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false K274sumo K274sumo sumoylated lysine sumoylated lysine modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli covalent binding covalent binding false false false kcnk1-sumo1-3 3209205 N/A 3209207 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 96-101 Region 96-101 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster N/A 3209208 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster proximity proximity close-contact colocalization neighbourhood interaction false false false kcnk1-ube2i-1 3209206 N/A 3209209 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster N/A 3209208 unspecified role unspecified role fluorescence donor fluorescence donor O00180:p.Lys274Gln K274Q mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster proximity proximity close-contact colocalization neighbourhood interaction false false false