DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ mbius-2010-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Möbius K., Arias-Cartin R., Breckau D., Hännig AL., Riedmann K., Biedendieck R., Schröder S., Becher D., Magalon A., Moser J., Jahn M., Jahn D. DIP original experiment: DIP-219808X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Heme biosynthesis is coupled to electron transport chains for energy generation. Möbius K., Arias-Cartin R., Breckau D., Hännig AL., Riedmann K., Biedendieck R., Schröder S., Becher D., Magalon A., Moser J., Jahn M., Jahn D. Only protein-protein interactions imex curation mbius-2010-2 ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Möbius K., Arias-Cartin R., Breckau D., Hännig AL., Riedmann K., Biedendieck R., Schröder S., Becher D., Magalon A., Moser J., Jahn M., Jahn D. DIP original experiment: DIP-219809X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Heme biosynthesis is coupled to electron transport chains for energy generation. Möbius K., Arias-Cartin R., Breckau D., Hännig AL., Riedmann K., Biedendieck R., Schröder S., Becher D., Magalon A., Moser J., Jahn M., Jahn D. Only protein-protein interactions imex curation mbius-2010-3 ecolx Escherichia coli pull down pull down de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Möbius K., Arias-Cartin R., Breckau D., Hännig AL., Riedmann K., Biedendieck R., Schröder S., Becher D., Magalon A., Moser J., Jahn M., Jahn D. DIP original experiment: DIP-219810X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Heme biosynthesis is coupled to electron transport chains for energy generation. Möbius K., Arias-Cartin R., Breckau D., Hännig AL., Riedmann K., Biedendieck R., Schröder S., Becher D., Magalon A., Moser J., Jahn M., Jahn D. Only protein-protein interactions imex curation hemg_ecoli Protoporphyrinogen IX dehydrogenase [quinone] Protoporphyrinogen IX dehydrogenase [menaquinone] Protoporphyrinogen IX dehydrogenase [ubiquinone] Protoporphyrinogen oxidase yihB hemG b3850 JW3827 protein protein ecoli Escherichia coli (strain K12) MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQWENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTNSYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTDWEQVANFAREIAHLTDKPTLK frda_ecoli Fumarate reductase flavoprotein subunit frdA Quinol-fumarate reductase flavoprotein subunit JW4115 b4154 protein protein ecoli Escherichia coli (strain K12) MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRSHTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGCPWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLDILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMALSHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLGEPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICELAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRLGSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIELGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG hemg-1 3209042 N/A 3209045 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false frda-hemg-1 3209043 N/A 3209046 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3209045 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false hemg-frda-1 3209044 N/A 3209046 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3209045 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false