IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ barbosa-2010-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Barbosa S., Pratte D., Schwarz H., Pipkorn R., Singer-Krüger B. birgit.singer-krueger@tuebingen.mpg.de Traffic (Copenhagen, Denmark) 2010 Only protein-protein interactions imex curation imex curation barbosa-2010-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom anti tag western anti tag western blot anti tag western Barbosa S., Pratte D., Schwarz H., Pipkorn R., Singer-Krüger B. birgit.singer-krueger@tuebingen.mpg.de Traffic (Copenhagen, Denmark) 2010 Only protein-protein interactions imex curation imex curation barbosa-2010-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Barbosa S., Pratte D., Schwarz H., Pipkorn R., Singer-Krüger B. birgit.singer-krueger@tuebingen.mpg.de Traffic (Copenhagen, Denmark) 2010 Only protein-protein interactions imex curation imex curation mon2_yeast Protein MON2 YNL297C N0453 MON2 YSL2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAMNTGGFDSMQRQLEAELRSLSSESKRRNSTIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSIPRSRLSEILDAFIEATHLAMEIQLKVLQVVPIFFKTYGKFIYGPLCKKLLLCCSNLLHVPNKAPVVVGTASATLQQLIDEIFDRLSIESVVDDKQYEVLISNSESIKVNVYRYDANKLFDNICSLNEISSNGAVSDEEMLLDIGDIPIDYGLEILESILKNSQKNLLECQDLQYLLRVKAIPLLLRCISSSRHFSTAVRSCRCLKLLIRKEYLSILELELEVILSLLIHGISVESNLSGWQRVLSLELFKDLSQDPEIVNTLYMDYDNYPDKKHVFKYLLKECIVLLNSPEYITFLAPSKVVEKMDSPLITTENSTVKTKFMHLLDKSNAPSINITYIISLILTICNHLCEGLNKSALESSPLEKKIEDKEREEGTGNDSTVVKVYSGLFSGLFELNKLFLYSTSLETSIFHLVVRAFQKLAHSAGVLSLKDKLRACMKLFSILITNNVTSSNQYSFNDTSKSAKNQHTRNISTSSVTTSPVESTKNPSRSIADSAQNKEMKRRLHPRNISSRQVSLLRALISLSISLGPIFDSESWRYTFLTWQWITYYIYGPSADFKESFYSEDIPPPPILTKSDVTSIESSLTKFFESTSSYSCSTFHLVLTRLILDSKNTLTLEQTNLNLNNDIGYHPLDAKDEIIPCIYNKAFFVNKIGELATYNCKKFLFGKNGKELWSLISTYMIKLISNREMDNDSLRLYTVRVFTDIIKKATNEVGNSDEQDNKVKQFGTLENLVIDSLMATINSIKQLDIGKQEIYNGTINVESDILFQLLLTLKEILNEFGELLMNSWTNIFNIINSPFEWTVEDTDFSVNEDIDDSSLFEGIVQKHKNMIQVSYDVFKLISDDFLQSLPMSVIKFVIDTLVNFVSQKRNLNISFSSISQFWLVGDYLRVRFNPETLNLSDEKRRSLSEKINNQKLIEIITSSSSHDWELYNGLWIYLLKNLINCTNDDRVEVKNGAVQTFFRIIDSHSVCFPPWDLIFLEVIEPLLTKEWSTEELENETDFINVTLQGLIKLYPEHFKDFKNNTTCAKEWSMLLDFLKRLLSSTSNNTKNAVILNYQTLLKEIITIEDVPSDILKKCCEIFTDYNITYSDLSTNASSKTEYDCIYELITGFPPLYQLISKYDAMTDEFVEKVLLLFNSAIKYPLLPEFVQDKTKPSSMQKAILSGLDIFMTNDSKDTEILILLQLSTISILAFDTREKITKKLGPKLPKASLNRLPTFEAISYMSCSNLRNRIAKIDQFGISTLKAKHILRILKNLAEIIKRKSLITGSESDEIPIWVLASNCFCDLSNKIFKSLQEDAENPLKDNFCDLFINVIVVTLQRINPELDNLTEIDDLNEYSKYREILLENRIIDLFNERQLDTFIYAVWDCSFLYEFDELENALMQDCGTFSELSQKLSSFDFSCIFGSTTNPRFQTKYKCSLECLQDLVNFMLNTNEKLRKLTAPYLSARIALALRRYISDEYLIGRAPIPKLRKTELATLLNGLCVILRGVLDQNSTLGNKQIGVENLQTLSPLILRTIPVSHKMDGLQDKVLELSLGFTKLD dop1_yeast Protein dopey DOP1 YD9302.17C YDR141C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLPLKPLTIDSNNKQLDSKQKKFRANVERALERFDSVTEWADYIASLGTLLKALQSWSPKFQNVRYYVPSPYQVSRRLTSSLSPALPAGVHQKTLEVYTYIFEHIGLETLATECNIWIPGILPLMTYASMSVRSHLIELYDNYILLLPQTTLRLLIRPLISSLLPGIDDESNDFLPLTLKLIETLQENLDDDSLFWQTLFLVMTANKGRRLGGLTWLTRKFPSLNAVPHLVNKIKMEAEENPSETETNDSHLDRKKRKEEAFKVLLPAAKDLVTPEPGLLIRCLVGCLEDENDILIKRSVLDLLLQRLRLDSPVLNVLITSEDKKLLIMSCCRTTLSKDMSLNRRIWNWLLGPTAGGMLNNNGGNSMEYTTSVKSANEESNVYFTKYGLSALLEGLSDLLSEEESVLTAFRISMAVMDRWEIGSLVIPELFIPLLYSSEKFKQNEQIMKTARTFFDNTETNIIWGKLFQELEDIKNLKILDFVLTNFNIGNDEEIIVRHLPLILLTLLALPSNDKDFDNIYKLQKFSLYNKLLNYIPERALLPLSHSKLKHDDEVSCEELLAKIRGFYTNVSNPSSILEKENIAERLPPFTTEDLTFLIADLIQKKLLSSLWDLENINESSKLFIAIFEKIPESEELKGRSHISWSDKKITQSIFEAIPRLCESNNDAKSEEIVGIVEIFGNYLYSRMEFIESMKLLKVVMMAVWKSLKDPRHQILGVKNLKTLNRFIPSKFIESALVYTFVEEEDISERLSVLDLLWTQLDSDSNLIRRPLELILGELFDDQNPFYLTVSKWILSILNSGSASRLFYILTDNILKVNRLEKERLDERDDLDMLTYEFQMLAYVLKTNNGRTRKVFSTELTSIKSSTIWKNEDVSTYKSLLLVTLMRFLNIKSNTHAKSIRSALILLDILLDGTEQNFKDIVIFLLQMSSKYIAEEGIEPELIAVSLLDIVSKVLRLSHDNGIKLDIFDDNAAHLKYIDFLVTSVSNMKSPLIVTAYVKLLSESIVYFENSIFRMILPLSASLVQCVQRLFLLEKREGGYYQPIALLLGGLEELLEISHGYLVTEEREGYFSGSNLKGDFIQSVVSNVFSSDSSNEESKIQGERDVILQSFRQVISCCLDIWYWAHNISCKSNDDSSLDATNHNSYKFKFRSKKLLETLFLLEPLELLENLISIRSDNTTVTLVHVLDGNKPAITIPHLLYGVIIRYNRTASVKFSNRDGSRSSTTKLTKGEPSMLKRLSGESIIAFLFNYVDSVENSAMEEFYGDFLLFFREVATNYNLYSDVSLSILKLVALISGKVSKTQFGEQKRVRREISDVFFKYLPNAFINFTNLYRGHPDSFKDLEFVVWRVQYIVNDQIGGDKFNTTLATIVNQCLTPYIKPKSEKTIPGYVLELAAVVSHLGSKVKSWRLLIAELFQNDKKLSVIGSDQTWEKIIYEWSIYPENKSKILNDLLLEIGSKRSSVTPTLITFNLGSDSEVEYKCQNLLKISYLLMVSPNDAYLLHFSSLISCIFHYLVSKDIKLKGSCWILLRVLLLRFSESHFNDYWSMISYCLQTNLQEFYESLQIQSEVDPQTILQVCKTLDLLLLLNMEGFTSTNEWIFVIDTINCVYKTNSFVALVDEIAEFKDYEITKTDDLELPTTLKDGLPLLRGIHKIERHTQLRSFFQNLSYLHYEKVYGLGSVDLYGCGEDLKKDILS 119459CD3785526C atc7_yeast Probable phospholipid-transporting ATPase NEO1 NEO1 YIL048W protein protein yeast Saccharomyces cerevisiae Baker's yeast MPNPPSFKSHKQNLFNSNNNQHANSVDSFDLHLDDSFDAALDSLQINNNPEPLSKHNTVGDRESFEMRTVDDLDNFSNHSSDSHRKSSNTDTHPLMYDNRLSQDDNFKFTNIASSPPSSSNNIFSKALSYLKVSNTKNWSKFGSPIELSDQHIEREIHPDTTPVYDRNRYVSNELSNAKYNAVTFVPTLLYEQFKFFYNLYFLVVALSQAVPALRIGYLSSYIVPLAFVLTVTMAKEAIDDIQRRRRDRESNNELYHVITRNRSIPSKDLKVGDLIKVHKGDRIPADLVLLQSSEPSGESFIKTDQLDGETDWKLRVACPLTQNLSENDLINRISITASAPEKSIHKFLGKVTYKDSTSNPLSVDNTLWANTVLASSGFCIACVVYTGRDTRQAMNTTTAKVKTGLLELEINSISKILCACVFALSILLVAFAGFHNDDWYIDILRYLILFSTIIPVSLRVNLDLAKSVYAHQIEHDKTIPETIVRTSTIPEDLGRIEYLLSDKTGTLTQNDMQLKKIHLGTVSYTSETLDIVSDYVQSLVSSKNDSLNNSKVALSTTRKDMSFRVRDMILTLAICHNVTPTFEDDELTYQAASPDEIAIVKFTESVGLSLFKRDRHSISLLHEHSGKTLNYEILQVFPFNSDSKRMGIIVRDEQLDEYWFMQKGADTVMSKIVESNDWLEEETGNMAREGLRTLVIGRKKLNKKIYEQFQKEYNDASLSMLNRDQQMSQVITKYLEHDLELLGLTGVEDKLQKDVKSSIELLRNAGIKIWMLTGDKVETARCVSISAKLISRGQYVHTITKVTRPEGAFNQLEYLKINRNACLLIDGESLGMFLKHYEQEFFDVVVHLPTVIACRCTPQQKADVALVIRKMTGKRVCCIGDGGNDVSMIQCADVGVGIVGKEGKQASLAADFSITQFCHLTELLLWHGRNSYKRSAKLAQFVMHRGLIIAICQAVYSICSLFEPIALYQGWLMVGYATCYTMAPVFSLTLDHDIEESLTKIYPELYKELTEGKSLSYKTFFVWVLLSLFQGSVIQLFSQAFTSLLDTDFTRMVAISFTALVVNELIMVALEIYTWNKTMLVTEIATLLFYIVSVPFLGDYFDLGYMTTVNYYAGLLVILLISIFPVWTAKAIYRRLHPPSYAKVQEFATP dop1-mon2-3 2955771 N/A 2955774 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2955775 unspecified role unspecified role bait bait n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2A, 9A dop1-neo1-2 2955771 N/A 2955776 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P40527:p.[Ile284Thr;Ile449Met;Glu605Gly;Leu680Pro;Tyr714His] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P40527:p.[Phe301Ser;Asp305Gly;Val373Ala;Ser539_Leu540delinsProPro;Asn631Ser;Gln655Arg;Val748Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 2955775 unspecified role unspecified role bait bait c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 3D DOP1 physically interacts with NEO1 in MON2-/- cells. dop1-dop1-1 2955771 N/A 2955775 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 2955775 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 9c, 9D dop1-neo1-3 2955771 N/A 2955776 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2955775 unspecified role unspecified role bait bait n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig.9B NEO1 physically interacts with all regions of DOP1 dop1-neo1-1 2955772 N/A 2955776 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2955775 unspecified role unspecified role bait bait c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 2B-E DOP1 physically interacts with NEO1 in YSl2-/- cells. dop1-dop1-2 2955773 N/A 2955775 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 2955775 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) 2955812 2955809 direct interaction direct interaction false false false Fig. 9 E