DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ boudreaux-2010-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Boudreaux DA., Maiti TK., Davies CW., Das C. DIP original experiment: DIP-219778X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Boudreaux DA., Maiti TK., Davies CW., Das C. Only protein-protein interactions imex curation boudreaux-2010-2 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Boudreaux DA., Maiti TK., Davies CW., Das C. DIP original experiment: DIP-219781X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Boudreaux DA., Maiti TK., Davies CW., Das C. Only protein-protein interactions imex curation boudreaux-2010-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Boudreaux DA., Maiti TK., Davies CW., Das C. DIP original experiment: DIP-219779X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Boudreaux DA., Maiti TK., Davies CW., Das C. Only protein-protein interactions imex curation boudreaux-2010-4 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Boudreaux DA., Maiti TK., Davies CW., Das C. DIP original experiment: DIP-219782X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Boudreaux DA., Maiti TK., Davies CW., Das C. Only protein-protein interactions imex curation boudreaux-2010-5 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Boudreaux DA., Maiti TK., Davies CW., Das C. DIP original experiment: DIP-219780X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Boudreaux DA., Maiti TK., Davies CW., Das C. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG uchl1_human Ubiquitin carboxyl-terminal hydrolase isozyme L1 Ubiquitin thioesterase L1 Neuron cytoplasmic protein 9.5 PGP 9.5 UCHL1 protein protein human Homo sapiens Human MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA ubiq_bovin Ubiquitin RPS27A UBA80 UBCEP1 UBA52 UBCEP2 UBB UBC protein protein bovin Bos taurus (Bovine) Bovine MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG hub-uchl1-1 3208344 N/A 3208349 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3208350 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hub-uchl1-2 3208345 N/A 3208349 enzyme target enzyme target substrate substrate unspecified role unspecified role AMC-label AMC-label fluorescent dye fluorescent dye label fluorescent dye c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false ecolx Escherichia coli N/A 3208350 enzyme enzyme unspecified role unspecified role P09936:p.Phe214Ala F214A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P09936:p.Cys90Ser C90S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli protein cleavage protein cleavage false false false hub-uchl1-3 3208346 N/A 3208349 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3208350 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hub-uchl1-4 3208347 N/A 3208349 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3208350 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false bub-uchl1-1 3208348 N/A 3208351 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3208350 unspecified role unspecified role unspecified role unspecified role P09936:p.Phe214Ala F214A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false