DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ geiger-2010-1 xenla Xenopus laevis (African clawed frog) African clawed frog bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. DIP original experiment: DIP-219718X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. Only protein-protein interactions imex curation geiger-2010-2 xenla Xenopus laevis (African clawed frog) African clawed frog bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. DIP original experiment: DIP-219714X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. Only protein-protein interactions imex curation geiger-2010-3 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress pull down pull down anti tag western anti tag western blot anti tag western Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. DIP original experiment: DIP-219720X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. Only protein-protein interactions imex curation geiger-2010-4 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. DIP original experiment: DIP-219716X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. Only protein-protein interactions imex curation geiger-2010-5 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. DIP original experiment: DIP-219722X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. Only protein-protein interactions imex curation geiger-2010-6 xenla Xenopus laevis (African clawed frog) African clawed frog bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. DIP original experiment: DIP-219719X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. Only protein-protein interactions imex curation geiger-2010-7 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. DIP original experiment: DIP-219721X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. Only protein-protein interactions imex curation geiger-2010-8 xenla Xenopus laevis (African clawed frog) African clawed frog bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. DIP original experiment: DIP-219715X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. Only protein-protein interactions imex curation geiger-2010-9 xenla Xenopus laevis (African clawed frog) African clawed frog bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. DIP original experiment: DIP-219717X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. Only protein-protein interactions imex curation geiger-2010-10 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. DIP original experiment: DIP-219723X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Geiger D., Scherzer S., Mumm P., Marten I., Ache P., Matschi S., Liese A., Wellmann C., Al-Rasheid KA., Grill E., Romeis T., Hedrich R. Only protein-protein interactions imex curation cdpkl_arath Calcium-dependent protein kinase 21 CPK21 T4B21.13 At4g04720 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCFSSKHRKTQNDGGEKSIPINPVQTHVVPEHRKPQTPTPKPMTQPIHQQISTPSSNPVSVRDPDTILGKPFEDIRKFYSLGKELGRGQFGITYMCKEIGTGNTYACKSILKRKLISKQDKEDVKREIQIMQYLSGQPNIVEIKGAYEDRQSIHLVMELCAGGELFDRIIAQGHYSERAAAGIIRSIVNVVQICHFMGVVHRDLKPENFLLSSKEENAMLKATDFGLSVFIEEGKVYRDIVGSAYYVAPEVLRRSYGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDEVIKGEIDFVSEPWPSISESAKDLVRKMLTKDPKRRITAAQVLEHPWIKGGEAPDKPIDSAVLSRMKQFRAMNKLKKLALKVIAESLSEEEIKGLKTMFANIDTDKSGTITYEELKTGLTRLGSRLSETEVKQLMEAADVDGNGTIDYYEFISATMHRYKLDRDEHVYKAFQHFDKDNSGHITRDELESAMKEYGMGDEASIKEVISEVDTDNDGRINFEEFCAMMRSGSTQPQGKLLPFH mint slac1_arath Guard cell S-type anion channel SLAC1 SLAC1 CDI3 OZS1 RCD3 F5O11.23 T12C24.3 At1g12480 Protein CARBON DIOXIDE INSENSITIVE 3 Protein OZONE-SENSITIVE 1 Protein RADICAL-INDUCED CELL DEATH 3 Protein SLOW ANION CHANNEL-ASSOCIATED 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MERKQSNAHSTFADINEVEDEAEQELQQQENNNNKRFSGNRGPNRGKQRPFRGFSRQVSLETGFSVLNRESRERDDKKSLPRSGRSFGGFESGGIINGGDGRKTDFSMFRTKSTLSKQKSLLPSIIRERDIENSLRTEDGETKDDSINENVSAGRYFAALRGPELDEVKDNEDILLPKEEQWPFLLRFPIGCFGICLGLSSQAVLWLALAKSPATNFLHITPLINLVVWLFSLVVLVSVSFTYILKCIFYFEAVKREYFHPVRVNFFFAPWVVCMFLAISVPPMFSPNRKYLHPAIWCVFMGPYFFLELKIYGQWLSGGKRRLCKVANPSSHLSVVGNFVGAILASKVGWDEVAKFLWAVGFAHYLVVFVTLYQRLPTSEALPKELHPVYSMFIAAPSAASIAWNTIYGQFDGCSRTCFFIALFLYISLVARINFFTGFKFSVAWWSYTFPMTTASVATIKYAEAVPGYPSRALALTLSFISTAMVCVLFVSTLLHAFVWQTLFPNDLAIAITKRKLTREKKPFKRAYDLKRWTKQALAKKISAEKDFEAEEESHH cdpk6_arath Calcium-dependent protein kinase 6 CPK6 CDPK3 Calcium-dependent protein kinase isoform CDPK3 Calmodulin-domain protein kinase CDPK isoform 6 F5J6.13 At2g17290 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGNSCRGSFKDKIYEGNHSRPEENSKSTTTTVSSVHSPTTDQDFSKQNTNPALVIPVKEPIMRRNVDNQSYYVLGHKTPNIRDLYTLSRKLGQGQFGTTYLCTDIATGVDYACKSISKRKLISKEDVEDVRREIQIMHHLAGHKNIVTIKGAYEDPLYVHIVMELCAGGELFDRIIHRGHYSERKAAELTKIIVGVVEACHSLGVMHRDLKPENFLLVNKDDDFSLKAIDFGLSVFFKPGQIFKDVVGSPYYVAPEVLLKHYGPEADVWTAGVILYILLSGVPPFWAETQQGIFDAVLKGYIDFDTDPWPVISDSAKDLIRKMLCSSPSERLTAHEVLRHPWICENGVAPDRALDPAVLSRLKQFSAMNKLKKMALKVIAESLSEEEIAGLRAMFEAMDTDNSGAITFDELKAGLRRYGSTLKDTEIRDLMEAADVDNSGTIDYSEFIAATIHLNKLEREEHLVSAFQYFDKDGSGYITIDELQQSCIEHGMTDVFLEDIIKEVDQDNDGRIDYEEFVAMMQKGNAGVGRRTMKNSLNISMRDV cdpkn_arath Calcium-dependent protein kinase 23 CPK23 T4B21.15 At4g04740 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCFSSKHRKTQNDGGGERSIPIIPVQTHIVDQVPDHRKPQIPSPSIPISVRDPETILGKPFEDIRKFYSLGRELGRGGLGITYMCKEIGTGNIYACKSILKRKLISELGREDVKTEIQIMQHLSGQPNVVEIKGSYEDRHSVHLVMELCAGGELFDRIIAQGHYSERAAAGTIKSIVDVVQICHLNGVIHRDLKPENFLFSSKEENAMLKVTDFGLSAFIEEGKIYKDVVGSPYYVAPEVLRQSYGKEIDIWSAGVILYILLCGVPPFWADNEEGVFVEILKCKIDFVREPWPSISDSAKDLVEKMLTEDPKRRITAAQVLEHPWIKGGEAPEKPIDSTVLSRMKQFRAMNKLKKLALKVSAVSLSEEEIKGLKTLFANMDTNRSGTITYEQLQTGLSRLRSRLSETEVQQLVEASDVDGNGTIDYYEFISATMHRYKLHHDEHVHKAFQHLDKDKNGHITRDELESAMKEYGMGDEASIKEVISEVDTDNDGKINFEEFRAMMRCGTTQPKGKQYPFH p2c56_arath Protein phosphatase 2C 56 Protein ABSCISIC ACID-INSENSITIVE 1 ABI1 At4g26080 F20B18.190 Protein phosphatase 2C ABI1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEVSPAIAGPFRPFSETQMDFTGIRLGKGYCNNQYSNQDSENGDLMVSLPETSSCSVSGSHGSESRKVLISRINSPNLNMKESAAADIVVVDISAGDEINGSDITSEKKMISRTESRSLFEFKSVPLYGFTSICGRRPEMEDAVSTIPRFLQSSSGSMLDGRFDPQSAAHFFGVYDGHGGSQVANYCRERMHLALAEEIAKEKPMLCDGDTWLEKWKKALFNSFLRVDSEIESVAPETVGSTSVVAVVFPSHIFVANCGDSRAVLCRGKTALPLSVDHKPDREDEAARIEAAGGKVIQWNGARVFGVLAMSRSIGDRYLKPSIIPDPEVTAVKRVKEDDCLILASDGVWDVMTDEEACEMARKRILLWHKKNAVAGDASLLADERRKEGKDPAAMSAAEYLSKLAIQRGSKDNISVVVVDLKPRRKLKSKPLN srk2e_arath Serine/threonine-protein kinase SRK2E SNRK2.6 Serine/threonine-protein kinase OST1 Protein OPEN STOMATA 1 SNF1-related kinase 2.6 F17I5.140 SRK2E OST1 At4g33950 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDRPAVSGPMDLPIMHDSDRYELVKDIGSGNFGVARLMRDKQSNELVAVKYIERGEKIDENVKREIINHRSLRHPNIVRFKEVILTPTHLAIVMEYASGGELFERICNAGRFSEDEARFFFQQLISGVSYCHAMQVCHRDLKLENTLLDGSPAPRLKICDFGYSKSSVLHSQPKSTVGTPAYIAPEVLLKKEYDGKVADVWSCGVTLYVMLVGAYPFEDPEEPKNFRKTIHRILNVQYAIPDYVHISPECRHLISRIFVADPAKRISIPEIRNHEWFLKNLPADLMNDNTMTTQFDESDQPGQSIEEIMQIIAEATVPPAGTQNLNHYLTGSLDIDDDMEEDLESDLDDLDIDSSGEIVYAM cpk21-slac1-1 3207104 N/A 3207114 unspecified role unspecified role unspecified role unspecified role N-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false xenla Xenopus laevis (African clawed frog) African clawed frog N/A 3207115 unspecified role unspecified role unspecified role unspecified role C-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false xenla Xenopus laevis (African clawed frog) African clawed frog direct interaction direct interaction false false false cpk6-slac1-1 3207105 N/A 3207116 unspecified role unspecified role unspecified role unspecified role N-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false xenla Xenopus laevis (African clawed frog) African clawed frog N/A 3207115 unspecified role unspecified role unspecified role unspecified role C-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false xenla Xenopus laevis (African clawed frog) African clawed frog direct interaction direct interaction false false false cpk23-slac1-1 3207106 N/A 3207117 unspecified role unspecified role bait bait Strep-tag Strep-tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3207115 unspecified role unspecified role prey prey N-terminal domain Region 1-186 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false cpk23-slac1-2 3207107 N/A 3207117 unspecified role unspecified role unspecified role unspecified role N-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3207115 unspecified role unspecified role unspecified role unspecified role C-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false cpk21-slac1-2 3207108 N/A 3207114 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3207115 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-186 Region 1-186 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false abi1-cpk21-1 3207109 N/A 3207114 unspecified role unspecified role unspecified role unspecified role N-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false xenla Xenopus laevis (African clawed frog) African clawed frog N/A 3207118 unspecified role unspecified role unspecified role unspecified role C-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false xenla Xenopus laevis (African clawed frog) African clawed frog direct interaction direct interaction false false false cpk23-slac1-3 3207110 N/A 3207117 enzyme enzyme unspecified role unspecified role Q9M101:p.Asp193Ala D193A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3207115 enzyme target enzyme target substrate substrate unspecified role unspecified role N-terminal domain Region 1-186 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false cpk23-slac1-4 3207111 N/A 3207117 unspecified role unspecified role unspecified role unspecified role N-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false xenla Xenopus laevis (African clawed frog) African clawed frog N/A 3207115 unspecified role unspecified role unspecified role unspecified role C-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N-terminal domain Region 1-186 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false xenla Xenopus laevis (African clawed frog) African clawed frog direct interaction direct interaction false false false abi1-cpk23-1 3207112 N/A 3207118 unspecified role unspecified role unspecified role unspecified role C-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false xenla Xenopus laevis (African clawed frog) African clawed frog N/A 3207117 unspecified role unspecified role unspecified role unspecified role N-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false xenla Xenopus laevis (African clawed frog) African clawed frog direct interaction direct interaction false false false slac1-srk2e-7 3207113 N/A 3207119 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3207115 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-186 Region 1-186 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false