DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ diskin-2010-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Diskin R., Marcovecchio PM., Bjorkman PJ. DIP original experiment: DIP-217070X Nature structural & molecular biology 2010 IMEx Structure of a clade C HIV-1 gp120 bound to CD4 and CD4-induced antibody reveals anti-CD4 polyreactivity. Diskin R., Marcovecchio PM., Bjorkman PJ. Only protein-protein interactions imex curation diskin-2010-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Diskin R., Marcovecchio PM., Bjorkman PJ. DIP original experiment: DIP-217071X Nature structural & molecular biology 2010 IMEx Structure of a clade C HIV-1 gp120 bound to CD4 and CD4-induced antibody reveals anti-CD4 polyreactivity. Diskin R., Marcovecchio PM., Bjorkman PJ. Only protein-protein interactions imex curation q1phm6_9hiv1 Envelope glycoprotein gp160 Env polyprotein env protein protein 9hiv1 Human immunodeficiency virus 1 MRVMGIQRNWQQWGIWGILGFWLLMICSGMGNLWVTVYYGVPVWKEAKTTLFCASDAKGYDTEVHNVWATHACVPTDPNPQEIVLENVTENFNMWKNDMVDQMHQDIISLWDQSLKPCVKLTPLCVTLNCSDATYNNGTNSTDTMKICSFNATTELRDKKKKEYALFYRLDIVPLKNESESQNFSEYILINCNTSTIAQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVVIRSENISNNVKTIIVHLNESVNITCIRPGNNTRRSIRIGPGQAFYAMGDIIGNIREAHCNISEKAWNETLKKVVEKLVKYFPNKTIEFAPPVGGDLEITTHSFNCGGEFFYCNTTKLFNSTHNSTDSTVNSTDSTAETGNSTNTNITLPCRIRQIINMWQEVGRAMYAPPSKGNITCISNITGLLLTRDGGENKTENNDTEIFRPGGGDMKDNWRSELYKYKVVEIKPLGVAPTRAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKSYGDIWDNMTWMQWDREINNYTNTIYRLLEDSQNQQEKNEQDLLALDKWQSLWSWFSISSWLWYIKIFIMVVGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTLPPNPRELDRLGGIEEEGGEQDRGRSVRLVSGFLPLAWDDLRSLCLFCYHRLRDLLLITTRAVELLARSILKGLQRGWEILKYLGSLVQYWGQELKKSAINLLDTTAIAVAEAADRILELLQRIWRGICNVPTRIRQGFEAALQ cd4_human T-cell surface glycoprotein CD4 CD4 T-cell surface antigen T4/Leu-3 protein protein human Homo sapiens Human MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMALIVLGGVAGLLLFIGLGIFFCVRCRHRRRQAERMSQIKRLLSEKKTCQCPHRFQKTCSPI cd4-env-6 3206599 N/A 3206601 unspecified role unspecified role unspecified role unspecified role GP120 protein Region 82-494 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3206602 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false cd4-env-7 3206600 N/A 3206601 unspecified role unspecified role unspecified role unspecified role GP120 protein Region 82-494 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3206602 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false