DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lee-2010-17 ecolx Escherichia coli comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie Lee BG., Park EY., Lee KE., Jeon H., Sung KH., Paulsen H., Rübsamen-Schaeff H., Brötz-Oesterhelt H., Song HK. DIP original experiment: DIP-217052X Nature structural & molecular biology 2010 IMEx Structures of ClpP in complex with acyldepsipeptide antibiotics reveal its activation mechanism. Lee BG., Park EY., Lee KE., Jeon H., Sung KH., Paulsen H., Rübsamen-Schaeff H., Brötz-Oesterhelt H., Song HK. Only protein-protein interactions imex curation lee-2010-18 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lee BG., Park EY., Lee KE., Jeon H., Sung KH., Paulsen H., Rübsamen-Schaeff H., Brötz-Oesterhelt H., Song HK. DIP original experiment: DIP-217049X Nature structural & molecular biology 2010 IMEx Structures of ClpP in complex with acyldepsipeptide antibiotics reveal its activation mechanism. Lee BG., Park EY., Lee KE., Jeon H., Sung KH., Paulsen H., Rübsamen-Schaeff H., Brötz-Oesterhelt H., Song HK. Only protein-protein interactions imex curation lee-2010-19 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lee BG., Park EY., Lee KE., Jeon H., Sung KH., Paulsen H., Rübsamen-Schaeff H., Brötz-Oesterhelt H., Song HK. DIP original experiment: DIP-217050X Nature structural & molecular biology 2010 IMEx Structures of ClpP in complex with acyldepsipeptide antibiotics reveal its activation mechanism. Lee BG., Park EY., Lee KE., Jeon H., Sung KH., Paulsen H., Rübsamen-Schaeff H., Brötz-Oesterhelt H., Song HK. Only protein-protein interactions imex curation lee-2010-20 ecolx Escherichia coli electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Lee BG., Park EY., Lee KE., Jeon H., Sung KH., Paulsen H., Rübsamen-Schaeff H., Brötz-Oesterhelt H., Song HK. DIP original experiment: DIP-217051X Nature structural & molecular biology 2010 IMEx Structures of ClpP in complex with acyldepsipeptide antibiotics reveal its activation mechanism. Lee BG., Park EY., Lee KE., Jeon H., Sung KH., Paulsen H., Rübsamen-Schaeff H., Brötz-Oesterhelt H., Song HK. Only protein-protein interactions imex curation clpp_bacsu ATP-dependent Clp protease proteolytic subunit clpP BSU34540 yvdN Endopeptidase Clp Caseinolytic protease Stress protein G7 protein protein bacsu Bacillus subtilis MNLIPTVIEQTNRGERAYDIYSRLLKDRIIMLGSAIDDNVANSIVSQLLFLAAEDPEKEISLYINSPGGSITAGMAIYDTMQFIKPKVSTICIGMAASMGAFLLAAGEKGKRYALPNSEVMIHQPLGGAQGQATEIEIAAKRILLLRDKLNKVLAERTGQPLEVIERDTDRDNFKSAEEALEYGLIDKILTHTEDKK 5D4D4BD548DC45A0 mint Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). ClpXP is involved in the complete degradation of the Site-2 clipped anti-sigma-W factor rsiW. This results in the release of sigW and the transcription activation of the genes under the control of the sigma-W factor bsclpp-1 3206218 N/A 3206222 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli covalent binding covalent binding false false false bsclpp-2 3206219 N/A 3206222 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false bsclpp-3 3206220 N/A 3206222 unspecified role unspecified role unspecified role unspecified role P80244:p.Ile20Ser I19S/C mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P80244:p.Leu24Ser L24S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P80244:p.Phe50Ser F49S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P80244:p.Ile20Cys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false bsclpp-4 3206221 N/A 3206222 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false