DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lupardus-2010-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lupardus PJ., Birnbaum ME., Garcia KC. DIP original experiment: DIP-216837X Structure (London, England : 1993) 2010 IMEx Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Lupardus PJ., Birnbaum ME., Garcia KC. Only protein-protein interactions imex curation lupardus-2010-2 trini Trichoplusia ni (Cabbage looper) Cabbage looper molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lupardus PJ., Birnbaum ME., Garcia KC. DIP original experiment: DIP-216835X Structure (London, England : 1993) 2010 IMEx Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Lupardus PJ., Birnbaum ME., Garcia KC. Only protein-protein interactions imex curation lupardus-2010-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lupardus PJ., Birnbaum ME., Garcia KC. DIP original experiment: DIP-216839X Structure (London, England : 1993) 2010 IMEx Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Lupardus PJ., Birnbaum ME., Garcia KC. Only protein-protein interactions imex curation lupardus-2010-4 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Lupardus PJ., Birnbaum ME., Garcia KC. DIP original experiment: DIP-216838X Structure (London, England : 1993) 2010 IMEx Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Lupardus PJ., Birnbaum ME., Garcia KC. Only protein-protein interactions imex curation lupardus-2010-5 trini Trichoplusia ni (Cabbage looper) Cabbage looper x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lupardus PJ., Birnbaum ME., Garcia KC. DIP original experiment: DIP-216836X Structure (London, England : 1993) 2010 IMEx Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Lupardus PJ., Birnbaum ME., Garcia KC. Only protein-protein interactions imex curation lupardus-2010-6 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Lupardus PJ., Birnbaum ME., Garcia KC. DIP original experiment: DIP-216840X Structure (London, England : 1993) 2010 IMEx Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Lupardus PJ., Birnbaum ME., Garcia KC. Only protein-protein interactions imex curation i13r2_human Interleukin-13 receptor subunit alpha-2 IL13RA2 IL13R Interleukin-13-binding protein protein protein human Homo sapiens Human MAFVCLAIGCLYTFLISTTFGCTSSSDTEIKVNPPQDFEIVDPGYLGYLYLQWQPPLSLDHFKECTVEYELKYRNIGSETWKTIITKNLHYKDGFDLNKGIEAKIHTLLPWQCTNGSEVQSSWAETTYWISPQGIPETKVQDMDCVYYNWQYLLCSWKPGIGVLLDTNYNLFYWYEGLDHALQCVDYIKADGQNIGCRFPYLEASDYKDFYICVNGSSENKPIRSSYFTFQLQNIVKPLPPVYLTFTRESSCEIKLKWSIPLGPIPARCFDYEIEIREDDTTLVTATVENETYTLKTTNETRQLCFVVRSKVNIYCSDDGIWSEWSDKQCWEGEDLSKKTLLRFWLPFGFILILVIFVTGLLLRKPNTYPKMIPEFFCDT il13_human Interleukin-13 IL13 NC30 protein protein human Homo sapiens Human MHPLLNPLLLALGLMALLLTTVIALTCLGGFASPGPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGRFN i13r1_human Interleukin-13 receptor subunit alpha-1 Cancer/testis antigen 19 IL13RA1 IL13R IL13RA protein protein human Homo sapiens Human MEWPARLCGLWALLLCAGGGGGGGGAAPTETQPPVTNLSVSVENLCTVIWTWNPPEGASSNCSLWYFSHFGDKQDKKIAPETRRSIEVPLNERICLQVGSQCSTNESEKPSILVEKCISPPEGDPESAVTELQCIWHNLSYMKCSWLPGRNTSPDTNYTLYYWHRSLEKIHQCENIFREGQYFGCSFDLTKVKDSSFEQHSVQIMVKDNAGKIKPSFNIVPLTSRVKPDPPHIKNLSFHNDDLYVQWENPQNFISRCLFYEVEVNNSQTETHNVFYVQEAKCENPEFERNVENTSCFMVPGVLPDTLNTVRIRVKTNKLCYEDDKLWSNWSQEMSIGKKRNSTLYITMLLIVPVIVAGAIIVLLLYLKRLKIIIFPPIPDPGKIFKEMFGDQNDDTLHWKKYDIYEKQTKEETDSVVLIENLKKASQ il13-il13ra2-1 3205857 N/A 3205863 unspecified role unspecified role unspecified role unspecified role Ectodomain Region 29-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3205864 unspecified role unspecified role unspecified role unspecified role Region 21-132 Region 21-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false il13-il13ra2-2 3205858 N/A 3205863 unspecified role unspecified role unspecified role unspecified role Ectodomain Region 29-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3205864 unspecified role unspecified role unspecified role unspecified role Region 21-132 Region 21-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false il13-il13ra1-4 3205859 N/A 3205865 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3205864 unspecified role unspecified role unspecified role unspecified role Region 21-132 Region 21-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false il13ra2-il13-1 3205860 N/A 3205863 unspecified role unspecified role bait bait Ectodomain Region 29-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3205864 unspecified role unspecified role prey prey P35225:p.Lys137Ala K137A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P35225:p.Phe140Ala F140A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Region 21-132 Region 21-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P35225:p.Ile47Ala I47A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P35225:p.Lys138Ala K138A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false il13-il13ra2-3 3205861 N/A 3205863 unspecified role unspecified role unspecified role unspecified role Ectodomain Region 29-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3205864 unspecified role unspecified role unspecified role unspecified role Region 21-132 Region 21-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false il13ra1-il13-1 3205862 N/A 3205865 unspecified role unspecified role bait bait trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3205864 unspecified role unspecified role prey prey Region 21-132 Region 21-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P35225:p.Ile47Ala I47A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P35225:p.Lys137Ala K137A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P35225:p.Phe140Ala F140A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P35225:p.Ile123Ala I123A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P35225:p.Glu124Ala E124A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P35225:p.Lys122Ala K122A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P35225:p.Met66Ala M66A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P35225:p.Arg141Ala R141A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false