DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ olsen-2010-3 in vitro In vitro comigration in sds comigration in sds page comigration in sds weight by staining molecular weight estimation by staining weight by staining Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. DIP original experiment: DIP-133671X Nature 2010 IMEx Active site remodelling accompanies thioester bond formation in the SUMO E1. Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. Only protein-protein interactions imex curation olsen-2010-4 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. DIP original experiment: DIP-133667X Nature 2010 IMEx Active site remodelling accompanies thioester bond formation in the SUMO E1. Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. Only protein-protein interactions imex curation olsen-2010-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. DIP original experiment: DIP-133669X Nature 2010 IMEx Active site remodelling accompanies thioester bond formation in the SUMO E1. Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. Only protein-protein interactions imex curation olsen-2010-6 in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. DIP original experiment: DIP-133674X Nature 2010 IMEx Active site remodelling accompanies thioester bond formation in the SUMO E1. Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. Only protein-protein interactions imex curation olsen-2010-7 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. DIP original experiment: DIP-133668X Nature 2010 IMEx Active site remodelling accompanies thioester bond formation in the SUMO E1. Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. Only protein-protein interactions imex curation olsen-2010-8 in vitro In vitro comigration in sds comigration in sds page comigration in sds weight by staining molecular weight estimation by staining weight by staining Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. DIP original experiment: DIP-133672X Nature 2010 IMEx Active site remodelling accompanies thioester bond formation in the SUMO E1. Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. Only protein-protein interactions imex curation olsen-2010-9 in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. DIP original experiment: DIP-133673X Nature 2010 IMEx Active site remodelling accompanies thioester bond formation in the SUMO E1. Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. Only protein-protein interactions imex curation olsen-2010-10 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. DIP original experiment: DIP-133670X Nature 2010 IMEx Active site remodelling accompanies thioester bond formation in the SUMO E1. Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. Only protein-protein interactions imex curation olsen-2010-11 in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. DIP original experiment: DIP-133675X Nature 2010 IMEx Active site remodelling accompanies thioester bond formation in the SUMO E1. Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. Only protein-protein interactions imex curation olsen-2010-12 in vitro In vitro comigration in sds comigration in sds page comigration in sds weight by staining molecular weight estimation by staining weight by staining Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. DIP original experiment: DIP-133676X Nature 2010 IMEx Active site remodelling accompanies thioester bond formation in the SUMO E1. Olsen SK., Capili AD., Lu X., Tan DS., Lima CD. Only protein-protein interactions imex curation smt3_yeast Ubiquitin-like protein SMT3 SMT3 D9719.15 YDR510W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGGATY A2790DE7F315E1A7 uba2_yeast Ubiquitin-activating enzyme E1-like UBA2 PIP2 UAL1 YDR390C D9509.10 SMT3-activating enzyme subunit 2 Polymerase-interacting protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPRETSLVTIIGEDSYKKLRSSRCLLVGAGGIGSELLKDIILMEFGEIHIVDLDTIDLSNLNRQFLFRQKDIKQPKSTTAVKAVQHFNNSKLVPYQGNVMDISTFPLHWFEQFDIIFNALDNLAARRYVNKISQFLSLPLIESGTAGFDGYMQPIIPGKTECFECTKKETPKTFPVCTIRSTPSQPIHCIVWAKNFLFNQLFASETSGNEDDNNQDWGTDDAEEIKRIKQETNELYELQKIIISRDASRIPEILNKLFIQDINKLLAIENLWKTRTKPVPLSDSQINTPTKTAQSASNSVGTIQEQISNFINITQKLMDRYPKEQNHIEFDKDDADTLEFVATAANIRSHIFNIPMKSVFDIKQIAGNIIPAIATTNAIVAGASSLISLRVLNLLKYAPTTKYTDLNMAFTAKASNLSQNRYLSNPKLAPPNKNCPVCSKVCRGVIKLSSDCLNKMKLSDFVVLIREKYSYPQDISLLDASNQRLLFDYDFEDLNDRTLSEINLGNGSIILFSDEEGDTMIRKAIELFLDVDDELPCNTCSLPDVEVPLIKANNSPSKNEEEEKNEKGADVVATTNSHGKDGIVILDDDEGEITIDAEPINGSKKRPVDTEISEAPSNKRTKLVNEPTNSDIVELD DBF800E1458B3B10 sae2_human SUMO-activating enzyme subunit 2 Ubiquitin-like modifier-activating enzyme 2 HRIHFB2115 Ubiquitin-like 1-activating enzyme E1B Anthracycline-associated resistance ARX UBA2 SAE2 UBLE1B protein protein human Homo sapiens Human MALSRGLPRELAEAVAGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFYPKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDWAEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLAEKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCALDPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQDDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELDDVIALD sae1_human SUMO-activating enzyme subunit 1 Ubiquitin-like 1-activating enzyme E1A SAE1 AOS1 UBLE1A SUA1 protein protein human Homo sapiens Human MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK sumo1_human Small ubiquitin-related modifier 1 SUMO1 Sentrin Ubiquitin-like protein SMT3C Ubiquitin-homology domain protein PIC1 Ubiquitin-like protein UBL1 GAP-modifying protein 1 SMT3 homolog 3 SMT3C SMT3H3 UBL1 OK/SW-cl.43 protein protein human Homo sapiens Human MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV ubiq-schpo Ubiquitin protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast This sequence has been withdrawn from Uniprot. uba1_schpo Ubiquitin-activating enzyme E1 1 ptr3 Poly(A)+ RNA transport protein 3 SPBC1604.21c SPBC211.09 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSNNMNIDQTDQNTIDEGLYSRQLYVLGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQPTRIEDLSSQYFLTEDDIGVPRAKVTVSKLAELNQYVPVSVVDELSTEYLKNFKCVVVTETSLTKQLEINDFTHKNHIAYIAADSRGLFGSIFCDFGENFICTDTDGNEPLTGMIASITDDGVVTMLEETRHGLENGDFVKFTEVKGMPGLNDGTPRKVEVKGPYTFSIGSVKDLGSAGYNGVFTQVKVPTKISFKSLRESLKDPEYVYPDFGKMMRPPQYHIAFQALSAFADAHEGSLPRPRNDIDAAEFFEFCKKIASTLQFDVELDEKLIKEISYQARGDLVAMSAFLGGAVAQEVLKATTSKFYPLKQYFYFDSLESLPSSVTISEETCKPRGCRYDGQIAVFGSEFQEKIASLSTFLVGAGAIGCEMLKNWAMMGVATGESGHISVTDMDSIEKSNLNRQFLFRPRDVGKLKSECASTAVSIMNPSLTGKITSYQERVGPESEGIFGDEFFEKLSLVTNALDNVEARMYVDRRCVFFEKPLLESGTLGTKGNTQVVVPHLTESYGSSQDPPEKSFPICTLKNFPNRIEHTIAWARDLFEGLFKQPIDNVNMYLSSPNFLETSLKTSSNPREVLENIRDYLVTEKPLSFEECIMWARLQFDKFFNNNIQQLLFNFPKDSVTSTGQPFWSGPKRAPTPLSFDIHNREHFDFIVAAASLYAFNYGLKSETDPAIYERVLAGYNPPPFAPKSGIKIQVNENEEAPETAANKDKQELKSIADSLPPPSSLVGFRLTPAEFEKDDDSNHHIDFITAASNLRAMNYDITPADRFKTKFVAGKIVPAMCTSTAVVSGLVCLELVKLVDGKKKIEEYKNGFFNLAIGLFTFSDPIASPKMKVNGKEIDKIWDRYNLPDCTLQELIDYFQKEEGLEVTMLSSGVSLLYANFQPPKKLAERLPLKISELVEQITKKKLEPFRKHLVLEICCDDANGEDVEVPFICIKL AB5207808ACC6C2D rhc31_yeast DNA damage tolerance protein RHC31 AOS1 RHC31 YPR180W P9705.5 RAD31 homolog protein protein yeast Saccharomyces cerevisiae Baker's yeast MDMKVEKLSEDEIALYDRQIRLWGMTAQANMRSAKVLLINLGAIGSEITKSIVLSGIGHLTILDGHMVTEEDLGSQFFIGSEDVGQWKIDATKERIQDLNPRIELNFDKQDLQEKDEEFFQQFDLVVATEMQIDEAIKINTLTRKLNIPLYVAGSNGLFAYVFIDLIEFISEDEKLQSVRPTTVGPISSNRSIIEVTTRKDEEDEKKTYERIKTKNCYRPLNEVLSTATLKEKMTQRQLKRVTSILPLTLSLLQYGLNQKGKAISFEQMKRDAAVWCENLGVPATVVKDDYIQQFIKQKGIEFAPVAAIIGGAVAQDVINILGKRLSPLNNFIVFDGITLDMPLFEF 1ADB7B817BA27F3E scsmt3-scuba2-1 3204578 N/A 3204588 unspecified role unspecified role unspecified role unspecified role Region 1-95 Region 1-95 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204589 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false sae1-uba2-3 3204579 N/A 3204590 unspecified role unspecified role unspecified role unspecified role Deletion of C-terminal domain Region 1-550 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204591 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false sae1-sumo1-1 3204580 N/A 3204592 unspecified role unspecified role unspecified role unspecified role Region 1-94 Region 1-94 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204590 unspecified role unspecified role unspecified role unspecified role Deletion of C-terminal domain Region 1-550 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204591 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false scsmt3-scuba2-2 3204581 N/A 3204588 unspecified role unspecified role unspecified role unspecified role Region 1-95 Region 1-95 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204589 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false sae1-sumo1-2 3204582 N/A 3204592 unspecified role unspecified role unspecified role unspecified role Region 1-94 Region 1-94 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204590 unspecified role unspecified role unspecified role unspecified role Deletion of C-terminal domain Region 1-550 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204591 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false spub-spuba1-1 3204583 N/A 3204593 unspecified role unspecified role unspecified role unspecified role Region 1-73 Region 1-73 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204594 unspecified role unspecified role unspecified role unspecified role O94609:p.Leu472Ala L472A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O94609:p.Arg474Ala R474A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Region 1-27 Region 1-27 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false O94609:p.Lys850Pro K850P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O94609:p.[Asp537Ala;Lys596Ala] D537A/K596A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O94609:p.Asp537Ala D537A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O94609:p.Asp465Ala D465A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O94609:p.Cys593Ala C593A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false spub-spuba1-2 3204584 N/A 3204593 unspecified role unspecified role unspecified role unspecified role Region 1-73 Region 1-73 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204594 unspecified role unspecified role unspecified role unspecified role O94609:p.Lys850Pro K850P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O94609:p.Asp537Ala D537A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O94609:p.Asp465Ala D465A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O94609:p.Cys593Ala C593A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false scaos1-scuba2-1 3204585 N/A 3204595 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3204589 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false sumo1-uba2-1 3204586 N/A 3204592 unspecified role unspecified role unspecified role unspecified role Region 1-94 Region 1-94 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204590 unspecified role unspecified role unspecified role unspecified role Q9UBT2:p.Arg59Ala R59A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Lys72Ala K72A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.[Gly381Pro;Asn382Pro] G381P/N382P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Asn382Pro N382P mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Asp117Ala D117A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Arg176Ala R176A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Cys173Ala C173A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Asp50Ala D50A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Asp50Glu D50E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sumo1-uba2-2 3204587 N/A 3204592 unspecified role unspecified role unspecified role unspecified role Region 1-94 Region 1-94 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204590 unspecified role unspecified role unspecified role unspecified role Q9UBT2:p.Arg59Ala R59A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Lys72Ala K72A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Leu57Ala L57A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Asn56Ala N56A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Asp117Ala D117A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.[Asp117Ala;Arg176Ala] D117A/R176A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.[Gly381Pro;Asn382Pro] G381P/N382P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Asp50Ala D50A/E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Cys173Ala C173A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UBT2:p.Asp50Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false