DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ gu-2010-4 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Gu M., Rajashankar KR., Lima CD. DIP original experiment: DIP-133881X Structure (London, England : 1993) 2010 IMEx Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA capping apparatus. Gu M., Rajashankar KR., Lima CD. Only protein-protein interactions imex curation gu-2010-5 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Gu M., Rajashankar KR., Lima CD. DIP original experiment: DIP-133883X Structure (London, England : 1993) 2010 IMEx Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA capping apparatus. Gu M., Rajashankar KR., Lima CD. Only protein-protein interactions imex curation gu-2010-6 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Gu M., Rajashankar KR., Lima CD. DIP original experiment: DIP-133882X Structure (London, England : 1993) 2010 IMEx Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA capping apparatus. Gu M., Rajashankar KR., Lima CD. Only protein-protein interactions imex curation mce1_yeast mRNA-capping enzyme subunit alpha CEG1 YGL130W G2853 mRNA guanylyltransferase GTP--RNA guanylyltransferase protein protein yeast Saccharomyces cerevisiae Baker's yeast MVLAMESRVAPEIPGLIQPGNVTQDLKMMVCKLLNSPKPTKTFPGSQPVSFQHSDVEEKLLAHDYYVCEKTDGLRVLMFIVINPVTGEQGCFMIDRENNYYLVNGFRFPRLPQKKKEELLETLQDGTLLDGELVIQTNPMTKLQELRYLMFDCLAINGRCLTQSPTSSRLAHLGKEFFKPYFDLRAAYPNRCTTFPFKISMKHMDFSYQLVKVAKSLDKLPHLSDGLIFTPVKAPYTAGGKDSLLLKWKPEQENTVDFKLILDIPMVEDPSLPKDDRNRWYYNYDVKPVFSLYVWQGGADVNSRLKHFDQPFDRKEFEILERTYRKFAELSVSDEEWQNLKNLEQPLNGRIVECAKNQETGAWEMLRFRDDKLNGNHTSVVQKVLESINDSVSLEDLEEIVGDIKRCWDERRANMAGGSGRPLPSQSQNATLSTSKPVHSQPPSNDKEPKYVDEDDWSD F7F5A3E59EEFA15F cet1_yeast mRNA-capping enzyme subunit beta CET1 mRNA 5'-phosphatase mRNA 5'-triphosphate monophosphatase P1433 YPL228W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSYTDNPPQTKRALSLDDLVNHDENEKVKLQKLSEAANGSRPFAENLESDINQTETGQAAPIDNYKESTGHGSHSQKPKSRKSSNDDEETDTDDEMGASGEINFDSEMDFDYDKQHRNLLSNGSPPMNDGSDANAKLEKPSDDSIHQNSKSDEEQRIPKQGNEGNIASNYITQVPLQKQKQTEKKIAGNAVGSVVKKEEEANAAVDNIFEEKATLQSKKNNIKRDLEVLNEISASSKPSKYRNVPIWAQKWKPTIKALQSINVKDLKIDPSFLNIIPDDDLTKSVQDWVYATIYSIAPELRSFIELEMKFGVIIDAKGPDRVNPPVSSQCVFTELDAHLTPNIDASLFKELSKYIRGISEVTENTGKFSIIESQTRDSVYRVGLSTQRPRFLRMSTDIKTGRVGQFIEKRHVAQLLLYSPKDSYDVKISLNLELPVPDNDPPEKYKSQSPISERTKDRVSYIHNDSCTRIDITKVENHNQNSKSRQSETTHEVELEINTPALLNAFDNITNDSKEYASLIRTFLNNGTIIRRKLSSLSYEIFEGSKKVM 2B946CC53D66F824 ceg1-cet1-2 3204472 N/A 3204475 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3204476 unspecified role unspecified role prey prey Triphosphatase domain Region 241-549 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false ceg1-cet1-3 3204473 N/A 3204475 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3204476 unspecified role unspecified role unspecified role unspecified role Triphosphatase domain Region 241-549 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ceg1-cet1-4 3204474 N/A 3204475 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3204476 unspecified role unspecified role unspecified role unspecified role Triphosphatase domain Region 241-549 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false