DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ sarma-2010-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Sarma GN., Kinderman FS., Kim C., von Daake S., Chen L., Wang BC., Taylor SS. DIP original experiment: DIP-133873X Structure (London, England : 1993) 2010 IMEx Structure of D-AKAP2:PKA RI complex: insights into AKAP specificity and selectivity. Sarma GN., Kinderman FS., Kim C., von Daake S., Chen L., Wang BC., Taylor SS. Only protein-protein interactions imex curation sarma-2010-2 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Sarma GN., Kinderman FS., Kim C., von Daake S., Chen L., Wang BC., Taylor SS. DIP original experiment: DIP-133876X Structure (London, England : 1993) 2010 IMEx Structure of D-AKAP2:PKA RI complex: insights into AKAP specificity and selectivity. Sarma GN., Kinderman FS., Kim C., von Daake S., Chen L., Wang BC., Taylor SS. Only protein-protein interactions imex curation sarma-2010-3 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sarma GN., Kinderman FS., Kim C., von Daake S., Chen L., Wang BC., Taylor SS. DIP original experiment: DIP-133874X Structure (London, England : 1993) 2010 IMEx Structure of D-AKAP2:PKA RI complex: insights into AKAP specificity and selectivity. Sarma GN., Kinderman FS., Kim C., von Daake S., Chen L., Wang BC., Taylor SS. Only protein-protein interactions imex curation sarma-2010-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sarma GN., Kinderman FS., Kim C., von Daake S., Chen L., Wang BC., Taylor SS. DIP original experiment: DIP-133875X Structure (London, England : 1993) 2010 IMEx Structure of D-AKAP2:PKA RI complex: insights into AKAP specificity and selectivity. Sarma GN., Kinderman FS., Kim C., von Daake S., Chen L., Wang BC., Taylor SS. Only protein-protein interactions imex curation kap0_bovin cAMP-dependent protein kinase type I-alpha regulatory subunit PRKAR1A protein protein bovin Bos taurus (Bovine) Bovine MASGTTASEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFEKLEKEEAKQIQNLQKAGSRADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV aka10_human A-kinase anchor protein 10, mitochondrial Protein kinase A-anchoring protein 10 Dual specificity A kinase-anchoring protein 2 AKAP10 protein protein human Homo sapiens Human MRGAGPSPRQSPRTLRPDPGPAMSFFRRKVKGKEQEKTSDVKSIKASISVHSPQKSTKNHALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEAAHFGDLGRSCLDYQTQETKSSLSKTLEQVLHDTIVLPYFIQFMELRRMEHLVKFWLEAESFHSTTWSRIRAHSLNTVKQSSLAEPVSPSKKHETTASFLTDSLDKRLEDSGSAQLFMTHSEGIDLNNRTNSTQNHLLLSQECDSAHSLRLEMARAGTHQVSMETQESSSTLTVASRNSPASPLKELSGKLMKSIEQDAVNTFTKYISPDAAKPIPITEAMRNDIIARICGEDGQVDPNCFVLAQSIVFSAMEQEHFSEFLRSHHFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLTAPGSVGPPDESHPGSSDSSASQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMTFGRVSDLGQFIRESEPEPDVRKSKGSMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDIMQQAQYDQPLEKSTKL prkar1a-1 3204417 N/A 3204421 unspecified role unspecified role unspecified role unspecified role Dimerization and docking domain Region 13-62 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false akap10-prkar1a-1 3204418 N/A 3204422 unspecified role unspecified role unspecified role unspecified role PKA-RII binding domain Region 623-650 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide c-term range C-terminal range c-term range c-term range C-terminal range c-term range false in vitro In vitro N/A 3204421 unspecified role unspecified role unspecified role unspecified role P00514:p.Cys17Ala C17A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P00514:p.Cys38Ala C38A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P00514:p.Tyr20Ala Y20A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P00514:p.His24Ala H24A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false prkar1a-2 3204419 N/A 3204421 unspecified role unspecified role unspecified role unspecified role Dimerization and docking domain Region 13-62 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false akap10-prkar1a-2 3204420 N/A 3204422 unspecified role unspecified role unspecified role unspecified role PKA-RII binding domain Region 623-662 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204421 unspecified role unspecified role unspecified role unspecified role Dimerization and docking domain Region 13-62 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false