DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ defeu-2010-1 baciu Bacillus subtilis tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. DIP original experiment: DIP-216018X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein. Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. Only protein-protein interactions imex curation defeu-2010-2 baciu Bacillus subtilis bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. DIP original experiment: DIP-216024X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein. Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. Only protein-protein interactions imex curation defeu-2010-3 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. DIP original experiment: DIP-216022X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein. Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. Only protein-protein interactions imex curation defeu-2010-4 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. DIP original experiment: DIP-216020X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein. Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. Only protein-protein interactions imex curation defeu-2010-5 baciu Bacillus subtilis tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. DIP original experiment: DIP-216019X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein. Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. Only protein-protein interactions imex curation defeu-2010-6 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. DIP original experiment: DIP-216023X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein. Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. Only protein-protein interactions imex curation defeu-2010-7 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. DIP original experiment: DIP-216021X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein. Defeu Soufo HJ., Reimold C., Linne U., Knust T., Gescher J., Graumann PL. Only protein-protein interactions imex curation eftu_bacsu Elongation factor Tu P-40 BSU01130 tuf tufA protein protein bacsu Bacillus subtilis MAKEKFDRSKSHANIGTIGHVDHGKTTLTAAITTVLHKKSGKGTAMAYDQIDGAPEERERGITISTAHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSKNVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEYDFPGDDVPVVKGSALKALEGDAEWEAKIFELMDAVDEYIPTPERDTEKPFMMPVEDVFSITGRGTVATGRVERGQVKVGDEVEIIGLQEENKKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREEIQRGQVLAKPGTITPHSKFKAEVYVLSKEEGGRHTPFFSNYRPQFYFRTTDVTGIIHLPEGVEMVMPGDNTEMNVELISTIAIEEGTRFSIREGGRTVGSGVVSTITE BC096F47AAE6F72A ch60_bacsu Chaperonin GroEL groEL 60 kDa chaperonin Chaperonin-60 groL mopA BSU06030 protein protein bacsu Bacillus subtilis MAKEIKFSEEARRAMLRGVDALADAVKVTLGPKGRNVVLEKKFGSPLITNDGVTIAKEIELEDAFENMGAKLVAEVASKTNDVAGDGTTTATVLAQAMIREGLKNVTAGANPVGVRKGMEQAVAVAIENLKEISKPIEGKESIAQVAAISAADEEVGSLIAEAMERVGNDGVITIEESKGFTTELEVVEGMQFDRGYASPYMVTDSDKMEAVLDNPYILITDKKITNIQEILPVLEQVVQQGKPLLLIAEDVEGEALATLVVNKLRGTFNAVAVKAPGFGDRRKAMLEDIAVLTGGEVITEDLGLDLKSTQIAQLGRASKVVVTKENTTIVEGAGETDKISARVTQIRAQVEETTSEFDREKLQERLAKLAGGVAVIKVGAATETELKERKLRIEDALNSTRAAVEEGIVSGGGTALVNVYNKVAAVEAEGDAQTGINIVLRALEEPIRQIAHNAGLEGSVIVERLKNEEIGVGFNAATGEWVNMIEKGIVDPTKVTRSALQNAASVAAMFLTTEAVVADKPEENGGGAGMPDMGGMGGMGGMM 25B72B4E88FF381D mreb_bacsu Cell shape-determining protein MreB mreB Actin-like MreB protein Rod shape-determining protein MreB BSU28030 protein protein bacsu Bacillus subtilis MFGIGARDLGIDLGTANTLVFVKGKGIVVREPSVVALQTDTKSIVAVGNDAKNMIGRTPGNVVALRPMKDGVIADYETTATMMKYYINQAIKNKGMFARKPYVMVCVPSGITAVEERAVIDATRQAGARDAYPIEEPFAAAIGANLPVWEPTGSMVVDIGGGTTEVAIISLGGIVTSQSIRVAGDEMDDAIINYIRKTYNLMIGDRTAEAIKMEIGSAEAPEESDNMEIRGRDLLTGLPKTIEITGKEISNALRDTVSTIVEAVKSTLEKTPPELAADIMDRGIVLTGGGALLRNLDKVISEETKMPVLIAEDPLDCVAIGTGKALEHIHLFKGKTR F554AAA82AD8853F mbl_bacsu Cell shape-determining protein Mbl Actin-like Mbl protein mbl Rod shape-determining protein Mbl BSU36410 protein protein bacsu Bacillus subtilis MFARDIGIDLGTANVLIHVKGKGIVLNEPSVVALDKNSGKVLAVGEEARRMVGRTPGNIVAIRPLKDGVIADFEVTEAMLKHFINKLNVKGLFSKPRMLICCPTNITSVEQKAIKEAAEKSGGKHVYLEEEPKVAAIGAGMEIFQPSGNMVVDIGGGTTDIAVISMGDIVTSSSIKMAGDKFDMEILNYIKREYKLLIGERTAEDIKIKVATVFPDARHEEISIRGRDMVSGLPRTITVNSKEVEEALRESVAVIVQAAKQVLERTPPELSADIIDRGVIITGGGALLNGLDQLLAEELKVPVLVAENPMDCVAIGTGVMLDNMDKLPKRKLS D1599B7EEDEAF8A9 efts_bacsu Elongation factor Ts tsf BSU16500 protein protein bacsu Bacillus subtilis MAITAQQVKELREKTGAGMMDCKKALTETDGDMDKAIDLLREKGIAKAAKKADRIAAEGSTLIKTDGNKGVILEVNSETDFVAKNEGFKELLNTLADHLLANTPADVEEAMGQKMENGSTVEEYITSAVAKIGEKITLRRFTVLTKDDSSAFGAYLHMGGRIGVLTVLNGTTDEETAKDIAMHVAAVNPRYISRDQVSEEETNHERQILTQQALQEGKPENIVAKMVEGRLNKFFEEICLLDQAFVKNPDEKVKQVIAAKNATVQTFVRYEVGEGIEKRQENFAEEVMNQVKK E26C2ECD4A0173EA mint rs2_bacsu 30S ribosomal protein S2 rpsB BS1 Vegetative protein 209 BSU16490 protein protein bacsu Bacillus subtilis MSVISMKQLLEAGVHFGHQTRRWNPKMKRYIFTERNGIYIIDLQKTVKKVEEAYNFTKNLAAEGGKILFVGTKKQAQDSVKEEAQRSGMYYVNQRWLGGTLTNFETIQKRIKRLKDIEKMQENGTFDVLPKKEVVQLKKELERLEKFLGGIKDMKDLPDALFIIDPRKERIAVAEARKLNIPIIGIVDTNCDPDEIDVVIPANDDAIRAVKLLTSKMADAILEAKQGEEEAEVAEETAPETETTTA 624D11EE6A12CAD5 mreb-ef_tu-1 3203087 N/A 3203094 unspecified role unspecified role prey prey baciu Bacillus subtilis N/A 3203095 unspecified role unspecified role prey prey baciu Bacillus subtilis N/A 3203096 unspecified role unspecified role bait bait TAP-tag TAP-tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false baciu Bacillus subtilis association association false false false ef_tu-mreb-1 3203088 N/A 3203094 unspecified role unspecified role unspecified role unspecified role C-terminal of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false baciu Bacillus subtilis N/A 3203096 unspecified role unspecified role unspecified role unspecified role N-terminal of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false baciu Bacillus subtilis direct interaction direct interaction false false false mbi-ef_tu-1 3203089 N/A 3203097 unspecified role unspecified role bait bait Strep-tag Strep-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3203094 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false mreb-ef_tu-2 3203090 N/A 3203094 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3203096 unspecified role unspecified role bait bait Strep-tag Strep-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false ef_tu-ef_ts-1 3203091 N/A 3203097 unspecified role unspecified role prey prey baciu Bacillus subtilis N/A 3203094 unspecified role unspecified role bait bait TAP-tag TAP-tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false baciu Bacillus subtilis N/A 3203098 unspecified role unspecified role prey prey baciu Bacillus subtilis N/A 3203099 unspecified role unspecified role prey prey baciu Bacillus subtilis N/A 3203096 unspecified role unspecified role prey prey baciu Bacillus subtilis association association false false false mbi-ef_tu-2 3203092 N/A 3203097 unspecified role unspecified role bait bait Strep-tag Strep-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3203094 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli direct interaction direct interaction false false false mreb-ef_tu-3 3203093 N/A 3203094 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3203096 unspecified role unspecified role bait bait Strep-tag Strep-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false