DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ an-2010-2 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined An JY., Kim EA., Jiang Y., Zakrzewska A., Kim DE., Lee MJ., Mook-Jung I., Zhang Y., Kwon YT. DIP original experiment: DIP-219201X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination. An JY., Kim EA., Jiang Y., Zakrzewska A., Kim DE., Lee MJ., Mook-Jung I., Zhang Y., Kwon YT. Only protein-protein interactions imex curation an-2010-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined An JY., Kim EA., Jiang Y., Zakrzewska A., Kim DE., Lee MJ., Mook-Jung I., Zhang Y., Kwon YT. DIP original experiment: DIP-219199X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination. An JY., Kim EA., Jiang Y., Zakrzewska A., Kim DE., Lee MJ., Mook-Jung I., Zhang Y., Kwon YT. Only protein-protein interactions imex curation an-2010-4 in vitro In vitro comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western An JY., Kim EA., Jiang Y., Zakrzewska A., Kim DE., Lee MJ., Mook-Jung I., Zhang Y., Kwon YT. DIP original experiment: DIP-219202X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination. An JY., Kim EA., Jiang Y., Zakrzewska A., Kim DE., Lee MJ., Mook-Jung I., Zhang Y., Kwon YT. Only protein-protein interactions imex curation an-2010-5 in vitro In vitro pull down pull down predetermined predetermined participant predetermined An JY., Kim EA., Jiang Y., Zakrzewska A., Kim DE., Lee MJ., Mook-Jung I., Zhang Y., Kwon YT. DIP original experiment: DIP-219200X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination. An JY., Kim EA., Jiang Y., Zakrzewska A., Kim DE., Lee MJ., Mook-Jung I., Zhang Y., Kwon YT. Only protein-protein interactions imex curation an-2010-6 in vitro In vitro comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western An JY., Kim EA., Jiang Y., Zakrzewska A., Kim DE., Lee MJ., Mook-Jung I., Zhang Y., Kwon YT. DIP original experiment: DIP-219203X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination. An JY., Kim EA., Jiang Y., Zakrzewska A., Kim DE., Lee MJ., Mook-Jung I., Zhang Y., Kwon YT. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG h2a3_human Histone H2A type 3 H2AW HIST3H2A protein protein human Homo sapiens Human MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK ube2b_human Ubiquitin-conjugating enzyme E2 B Ubiquitin-protein ligase B Ubiquitin carrier protein B RAD6 homolog B Ubiquitin-conjugating enzyme E2-17 kDa UBE2B RAD6B E2 ubiquitin-conjugating enzyme B protein protein human Homo sapiens Human MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS d5mth0_rat E3 ubiquitin-protein ligase Ubr2 UBR2 protein protein rat Rattus norvegicus (Rat) Rat MASEMEPEVQAIDRSLLECSAEEIAGKWLQATDLTREVYQHLAHCVPKIYCQGPNPFPQKEDTLAQHILLGPMEWYICGEDPALGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMQCFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQRHELSSSEVVEEEDPLVHLSEDVIARTYNIFAIMFRYAVDMLTWEKESELPEDLEVGEKTDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCDQAKTVIVRNTSRQTKPLKVQVMHSSVAAHQNFGLKALSWLGSVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYERLQRDYVTDDHDREFSVADLSVQIFTVPSLARMLITEENLMTVIIKAFMDHLKHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDELRQKFLQGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMVQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPISRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEVTHKDVVQQNNTLIEEMLYLIIMLVGERFSPGVGQVGATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIETVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRENKEDTALPPPALPPFCPLFGSLVNILQCDVMLSIMGTILQWAVERHGSAWSESMLQRVLHLIGMALQEEKHHLENAVEGHVQTFTFTQKISKPGDAPNNSPSILAMLETLQNAPSLEVHKDMIRWLLKMFNTIKKIRESSSSSPVAEAEGAIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTIELDASASAALDNSPLVPDAALTALGPAQTQVPEPRQFVTCILCQEEQEVTVESRAMVLAAFVQRSTVLSKDRSKVIEDPEKYDPLFMHPDLSCGTHTGSCGHAMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRSILSRRLNFSDQPDLAQWTRTIAQQIKVIQMLRKGDSAADKPSSEDTEDMNIIPIPEGFRPDFYPRNPYSDSIKEMLTTFGTATYKVGLKVHPNEDDPRVPIMCWGTCAYTIQSIERILSEEEKPVFGPLPCRLDDCLRSLTRFAAAHWTVALLPVVQGHFCKLFASLVPSDGSEDLPCILDIDMFHLLVGLVLSFPALQCQDFSGSSLATGDLHIFHLVTLAHIVQILLTSCTEENGMDQENPTGEEELAILSLHKTLHRCTGSALDEAPSGWHLWRSVRAGILPFLKCSALFFHYLNGVPAPPDLQVSGASHFEHLCNYLSLPTNLIRLFQENSDIMNSLIESWCQNSEVKRYLNGERGAISYPRGANKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGSLLCSQSYCCQAELEGEDVGACTAHTYSCGSGAGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCQERFRKIQKLWQQHSITEEIGHAQEANQTLVGIDWQHL hist3h2a-ub-1 3202313 N/A 3202318 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3202319 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ube2b-hist3h2a-1 3202314 N/A 3202320 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3202321 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat N/A 3202319 unspecified role unspecified role prey prey ecolx Escherichia coli association association false false false hist3h2a-ub-2 3202315 N/A 3202318 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli N/A 3202319 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ube2b-hist3h2a-2 3202316 N/A 3202319 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3202320 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false hist3h2a-ub-3 3202317 N/A 3202318 unspecified role unspecified role unspecified role unspecified role HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli N/A 3202319 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false