DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ abankwa-2010-1 crisp Cricetidae sp. Hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Abankwa D., Gorfe AA., Inder K., Hancock JF. DIP original experiment: DIP-219192X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ras membrane orientation and nanodomain localization generate isoform diversity. Abankwa D., Gorfe AA., Inder K., Hancock JF. Only protein-protein interactions imex curation abankwa-2010-2 crisp Cricetidae sp. Hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Abankwa D., Gorfe AA., Inder K., Hancock JF. DIP original experiment: DIP-219197X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ras membrane orientation and nanodomain localization generate isoform diversity. Abankwa D., Gorfe AA., Inder K., Hancock JF. Only protein-protein interactions imex curation abankwa-2010-3 crisp Cricetidae sp. Hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Abankwa D., Gorfe AA., Inder K., Hancock JF. DIP original experiment: DIP-219194X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ras membrane orientation and nanodomain localization generate isoform diversity. Abankwa D., Gorfe AA., Inder K., Hancock JF. Only protein-protein interactions imex curation abankwa-2010-4 crisp Cricetidae sp. Hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Abankwa D., Gorfe AA., Inder K., Hancock JF. DIP original experiment: DIP-219196X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ras membrane orientation and nanodomain localization generate isoform diversity. Abankwa D., Gorfe AA., Inder K., Hancock JF. Only protein-protein interactions imex curation abankwa-2010-5 crisp Cricetidae sp. Hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Abankwa D., Gorfe AA., Inder K., Hancock JF. DIP original experiment: DIP-219193X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ras membrane orientation and nanodomain localization generate isoform diversity. Abankwa D., Gorfe AA., Inder K., Hancock JF. Only protein-protein interactions imex curation abankwa-2010-6 crisp Cricetidae sp. Hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Abankwa D., Gorfe AA., Inder K., Hancock JF. DIP original experiment: DIP-219195X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ras membrane orientation and nanodomain localization generate isoform diversity. Abankwa D., Gorfe AA., Inder K., Hancock JF. Only protein-protein interactions imex curation pk3ca_bovin Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Phosphoinositide-3-kinase catalytic alpha polypeptide Serine/threonine protein kinase PIK3CA PIK3CA Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha protein protein bovin Bos taurus (Bovine) Bovine MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLITIKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPMDCFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLRAICTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQHALN rash_human GTPase HRas HRAS HRAS1 Transforming protein p21 p21ras H-Ras-1 c-H-ras Ha-Ras protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS p01116-2 GTPase KRas KRAS KRAS2 RASK2 K-Ras 2 Ki-Ras c-K-ras c-Ki-ras K-Ras4B protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM Variant in position: 152:V->G (in NS3). Variant in position: 153:D->V (in CFC2 and NS3, exhibits only minor alterations in its in vitro biochemical behavior compared to wild-type protein). Variant in position: 156:F->I (in NS3/CFC2). Variant in position: 156:F->L (found in a patient with Costello syndrome, exhibits an increase in intrinsic and guanine nucleotide exchange factor catalyzed nucleotide exchange in combination with an impaired GTPase-activating protein-stimulated GTP hydrolysis but functional in interaction with effectors). raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF rasm_human Ras-related protein M-Ras MRAS RRAS3 Ras-related protein R-Ras3 protein protein human Homo sapiens Human MATSAVPSDNLPTYKLVVVGDGGVGKSALTIQFFQKIFVPDYDPTIEDSYLKHTEIDNQWAILDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEHVDRFHQLILRVKDRESFPMILVANKVDLMHLRKITREQGKEMATKHNIPYIETSAKDPPLNVDKAFHDLVRVIRQQIPEKSQKKKKKTKWRGDRATGTHKLQCVIL leg1_rat Galectin-1 Lectin galactoside-binding soluble 1 Beta-galactoside-binding lectin L-14-I S-Lac lectin 1 Galaptin 14 kDa lectin RL 14.5 Lgals1 Lactose-binding lectin 1 protein protein rat Rattus norvegicus (Rat) Rat MACGLVASNLNLKPGECLKVRGELAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDDGTWGTEQRETAFPFQPGSITEVCITFDQADLTIKLPDGHEFKFPNRLNMEAINYMAADGDFKIKCVAFE rasn_human GTPase NRas Transforming protein N-Ras NRAS HRAS1 protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKKLNSSDDGTQGCMGLPCVVM hras-pik3ca-3 3201909 N/A 3201915 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept RBD domain Region 173-292 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crisp Cricetidae sp. Hamster N/A 3201916 unspecified role unspecified role fluorescence donor fluorescence donor GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P01112:p.[Arg169Ala;Lys170Ala] R169A/K170A mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false crisp Cricetidae sp. Hamster direct interaction direct interaction false false false kras-raf1-3 3201910 N/A 3201917 unspecified role unspecified role fluorescence donor fluorescence donor GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P01116-2:p.[Lys128Ala;Arg135Ala] K128A/R135A mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false crisp Cricetidae sp. Hamster N/A 3201918 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept RBD-CRD domain Region 51-200 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crisp Cricetidae sp. Hamster direct interaction direct interaction false false false mras-raf1-1 3201911 N/A 3201919 unspecified role unspecified role fluorescence donor fluorescence donor GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crisp Cricetidae sp. Hamster N/A 3201918 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept RBD-CRD domain Region 51-200 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crisp Cricetidae sp. Hamster direct interaction direct interaction false false false hras-raf1-7 3201912 N/A 3201918 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept RBD-CRD domain Region 51-200 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crisp Cricetidae sp. Hamster N/A 3201916 unspecified role unspecified role fluorescence donor fluorescence donor GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crisp Cricetidae sp. Hamster direct interaction direct interaction false false false hras-lgals1-1 3201913 N/A 3201920 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crisp Cricetidae sp. Hamster N/A 3201916 unspecified role unspecified role fluorescence donor fluorescence donor GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P01112:p.[Arg169Ala;Lys170Ala] R169A/K170A mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false crisp Cricetidae sp. Hamster direct interaction direct interaction false false false nras-raf1-1 3201914 N/A 3201921 unspecified role unspecified role fluorescence donor fluorescence donor GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P01111:p.[Lys128Ala;Lys135Ala] K128A/K135A mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P01111:p.[Lys169Ala;Lys170Ala] K169A/K170A mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false crisp Cricetidae sp. Hamster N/A 3201918 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept RBD-CRD domain Region 51-200 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crisp Cricetidae sp. Hamster direct interaction direct interaction false false false