DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhou-2010-8 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhou Q., Kapoor M., Guo M., Belani R., Xu X., Kiosses WB., Hanan M., Park C., Armour E., Do MH., Nangle LA., Schimmel P., Yang XL. DIP original experiment: DIP-133963X Nature structural & molecular biology 2010 IMEx Orthogonal use of a human tRNA synthetase active site to achieve multifunctionality. Zhou Q., Kapoor M., Guo M., Belani R., Xu X., Kiosses WB., Hanan M., Park C., Armour E., Do MH., Nangle LA., Schimmel P., Yang XL. Only protein-protein interactions imex curation zhou-2010-13 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhou Q., Kapoor M., Guo M., Belani R., Xu X., Kiosses WB., Hanan M., Park C., Armour E., Do MH., Nangle LA., Schimmel P., Yang XL. DIP original experiment: DIP-133966X Nature structural & molecular biology 2010 IMEx Orthogonal use of a human tRNA synthetase active site to achieve multifunctionality. Zhou Q., Kapoor M., Guo M., Belani R., Xu X., Kiosses WB., Hanan M., Park C., Armour E., Do MH., Nangle LA., Schimmel P., Yang XL. Only protein-protein interactions imex curation zhou-2010-14 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhou Q., Kapoor M., Guo M., Belani R., Xu X., Kiosses WB., Hanan M., Park C., Armour E., Do MH., Nangle LA., Schimmel P., Yang XL. DIP original experiment: DIP-133965X Nature structural & molecular biology 2010 IMEx Orthogonal use of a human tRNA synthetase active site to achieve multifunctionality. Zhou Q., Kapoor M., Guo M., Belani R., Xu X., Kiosses WB., Hanan M., Park C., Armour E., Do MH., Nangle LA., Schimmel P., Yang XL. Only protein-protein interactions imex curation zhou-2010-15 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhou Q., Kapoor M., Guo M., Belani R., Xu X., Kiosses WB., Hanan M., Park C., Armour E., Do MH., Nangle LA., Schimmel P., Yang XL. DIP original experiment: DIP-133964X Nature structural & molecular biology 2010 IMEx Orthogonal use of a human tRNA synthetase active site to achieve multifunctionality. Zhou Q., Kapoor M., Guo M., Belani R., Xu X., Kiosses WB., Hanan M., Park C., Armour E., Do MH., Nangle LA., Schimmel P., Yang XL. Only protein-protein interactions imex curation zhou-2010-16 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhou Q., Kapoor M., Guo M., Belani R., Xu X., Kiosses WB., Hanan M., Park C., Armour E., Do MH., Nangle LA., Schimmel P., Yang XL. DIP original experiment: DIP-133967X Nature structural & molecular biology 2010 IMEx Orthogonal use of a human tRNA synthetase active site to achieve multifunctionality. Zhou Q., Kapoor M., Guo M., Belani R., Xu X., Kiosses WB., Hanan M., Park C., Armour E., Do MH., Nangle LA., Schimmel P., Yang XL. Only protein-protein interactions imex curation cadh5_human Cadherin-5 CDH5 Vascular endothelial cadherin 7B4 antigen protein protein human Homo sapiens Human MQRLMMLLATSGACLGLLAVAAVAAAGANPAQRDTHSLLPTHRRQKRDWIWNQMHIDEEKNTSLPHHVGKIKSSVSRKNAKYLLKGEYVGKVFRVDAETGDVFAIERLDRENISEYHLTAVIVDKDTGENLETPSSFTIKVHDVNDNWPVFTHRLFNASVPESSAVGTSVISVTAVDADDPTVGDHASVMYQILKGKEYFAIDNSGRIITITKSLDREKQARYEIVVEARDAQGLRGDSGTATVLVTLQDINDNFPFFTQTKYTFVVPEDTRVGTSVGSLFVEDPDEPQNRMTKYSILRGDYQDAFTIETNPAHNEGIIKPMKPLDYEYIQQYSFIVEATDPTIDLRYMSPPAGNRAQVIINITDVDEPPIFQQPFYHFQLKENQKKPLIGTVLAMDPDAARHSIGYSIRRTSDKGQFFRVTKKGDIYNEKELDREVYPWYNLTVEAKELDSTGTPTGKESIVQVHIEVLDENDNAPEFAKPYQPKVCENAVHGQLVLQISAIDKDITPRNVKFKFILNTENNFTLTDNHDNTANITVKYGQFDREHTKVHFLPVVISDNGMPSRTGTSTLTVAVCKCNEQGEFTFCEDMAAQVGVSIQAVVAILLCILTITVITLLIFLRRRLRKQARAHGKSVPEIHEQLVTYDEEGGGEMDTTSYDVSVLNSVRRGGAKPPRPALDARPSLYAQVQKPPRHAPGAHGGPGEMAAMIEVKKDEADHDGDGPPYDTLHIYGYEGSESIAESLSSLGTDSSDSDVDYDFLNDWGPRFKMLAELYGSDPREELLY sywc_human Tryptophan--tRNA ligase, cytoplasmic WARS1 IFI53 Interferon-induced protein 53 Tryptophanyl-tRNA synthetase WARS WRS protein protein human Homo sapiens Human MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGGNCDVDVSFMYLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRKEVTDEIVKEFMTPRKLSFDFQ cadh5_bovin Cadherin-5 CDH5 Vascular endothelial cadherin protein protein bovin Bos taurus (Bovine) Bovine MQALVMLLATGATYYLGLLAAAAAAVNPGRPNTPGSLPAHRRQKRDWIWNQMHIDEERNDSLPHYVGKIKSSVDPKKTEYQLRGESAGKVFRVDKNTGDVYALERLDREKISEYHLTALVVDKDSKKNLESPSSFTIKVHDVNDNWPVFTHRVFNASVPEMSGIGTSVIQVTAMDADDPTVADHASVVYQLTKGKENFDIRGSGLIVTMNKHLDRETQDKYEVVVKAEDAQGRRGESGTATVFITLQDVNDNFPIFTQNRYTFSVPEDIRVGSPLGSLFVEDPDEPQNRKTKYSFVQGEYRDTFTIDTDPSHNEGIIKPIKPLDYERIRQYSFTIEATDPTIDLRYLGSTSPKNIARVIINVTDVDEPPIFQQPFYHFQLQENQKKPLIGSVLAKDPDAAQRDIRYSIRRTSDKGQFFGITKKGGIYNDKELDREVYPWYNLTVEAKEVDLSGTPTGKESIVQVHIEVMDENDNAPEFAKPYEPKVCENAPQGKLVVQISATDKDITPRDVKFKFSLSTEDSNFTLIDNHDNTANIIVKYGYFDRERAKVHHLPVLISDNGRPSLTGTSTLHVTVCKCNEHGEFTLCEEAAAQVGISIQALVAIFLCILTFTVITLLIILRRRLRKQARAHGKSVPEIHEQLVTYDEEGGGEMDTTSYDVSVLNSARHGGAKPPRPALDARPSLYAQVQKPPRQAPGAHAPGEMAAMIGVKKDEADHDGGGPPYDTLHIYGYEGAESIAESLSSLGTDSSDSDIDYDFLNDWGTRFKMLAELYGSDPQEELVY cdh5-wars-1 3200907 N/A 3200912 unspecified role unspecified role bait bait Extracellular domain Region 48-593 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Fc-tag Fc-tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3200913 unspecified role unspecified role prey prey T2-TrpRS subunit Region 94-471 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false cdh5-wars-2 3200908 N/A 3200912 unspecified role unspecified role bait bait Extracellular domain Region 48-593 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Fc-tag Fc-tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P33151:p.Trp51Ala W51A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P33151:p.Trp49Ala W49A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P33151:p.[Trp49Ala;Trp51Ala] W49A/W51A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3200913 unspecified role unspecified role prey prey T2-TrpRS subunit Region 94-471 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false cdh5-wars-3 3200909 N/A 3200912 unspecified role unspecified role bait bait Extracellular domain Region 48-593 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Fc-tag Fc-tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse Mus musculus Mouse N/A 3200913 unspecified role unspecified role prey prey T2-TrpRS subunit Region 94-471 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdh5-wars-4 3200910 N/A 3200912 unspecified role unspecified role bait bait Extracellular domain Region 48-593 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Fc-tag Fc-tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3200913 unspecified role unspecified role prey prey T2-TrpRS subunit Region 94-471 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false P23381:p.Ala310Trp A310W mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P23381:p.Gly172Met G172M mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P23381:p.Gly161Trp G161W mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P23381:p.[Tyr159Ala;Gln194Ala] Y159A/Q194A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false wars-cdh5-1 3200911 N/A 3200914 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine N/A 3200913 unspecified role unspecified role bait bait T2-TrpRS subunit Region 94-471 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false