DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ganesan-2009-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. DIP original experiment: DIP-132190X Structure (London, England : 1993) 2009 IMEx Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. Only protein-protein interactions imex curation ganesan-2009-2 in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa tag visualisation tag visualisation Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. DIP original experiment: DIP-132193X Structure (London, England : 1993) 2009 IMEx Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. Only protein-protein interactions imex curation ganesan-2009-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. DIP original experiment: DIP-132191X Structure (London, England : 1993) 2009 IMEx Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. Only protein-protein interactions imex curation ganesan-2009-4 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. DIP original experiment: DIP-132192X Structure (London, England : 1993) 2009 IMEx Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. Only protein-protein interactions imex curation kqlr KQLR inhibitor peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KQLR hgfa_human Hepatocyte growth factor activator HGFAC protein protein human Homo sapiens Human MGRWAWVPSPWPPPGLGPFLLLLLLLLLLPRGFQPQPGGNRTESPEPNATATPAIPTILVTSVTSETPATSAPEAEGPQSGGLPPPPRAVPSSSSPQAQALTEDGRPCRFPFRYGGRMLHACTSEGSAHRKWCATTHNYDRDRAWGYCVEATPPPGGPAALDPCASGPCLNGGSCSNTQDPQSYHCSCPRAFTGKDCGTEKCFDETRYEYLEGGDRWARVRQGHVEQCECFGGRTWCEGTRHTACLSSPCLNGGTCHLIVATGTTVCACPPGFAGRLCNIEPDERCFLGNGTGYRGVASTSASGLSCLAWNSDLLYQELHVDSVGAAALLGLGPHAYCRNPDNDERPWCYVVKDSALSWEYCRLEACESLTRVQLSPDLLATLPEPASPGRQACGRRHKKRTFLRPRIIGGSSSLPGSHPWLAAIYIGDSFCAGSLVHTCWVVSAAHCFSHSPPRDSVSVVLGQHFFNRTTDVTQTFGIEKYIPYTLYSVFNPSDHDLVLIRLKKKGDRCATRSQFVQPICLPEPGSTFPAGHKCQIAGWGHLDENVSGYSSSLREALVPLVADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLACEKNGVAYLYGIISWGDGCGRLHKPGVYTRVANYVDWINDRIRPPRRLVAPS o43278-1 Kunitz-type protease inhibitor 1 UNQ223/PRO256 HAI-1B Hepatocyte growth factor activator inhibitor type 1 SPINT1 HAI1 protein protein human Homo sapiens Human MAPARTMARARLAPAGIPAVALWLLCTLGLQGTQAGPPPAPPGLPAGADCLNSFTAGVPGFVLDTNASVSNGATFLESPTVRRGWDCVRACCTTQNCNLALVELQPDRGEDAIAACFLINCLYEQNFVCKFAPREGFINYLTREVYRSYRQLRTQGFGGSGIPKAWAGIDLKVQPQEPLVLKDVENTDWRLLRGDTDVRVERKDPNQVELWGLKEGTYLFQLTVTSSDHPEDTANVTVTVLSTKQTEDYCLASNKVGRCRGSFPRWYYDPTEQICKSFVYGGCLGNKNNYLREEECILACRGVQGGPLRGSSGAQATFPQGPSMERRHPVCSGTCQPTQFRCSNGCCIDSFLECDDTPNCPDASDEAACEKYTSGFDELQRIHFPSDKGHCVDLPDTGLCKESIPRWYYNPFSEHCARFTYGGCYGNKNNFEEEQQCLESCRGISKKDVFGLRREIPIPSTGSVEMAVAVFLVICIVVVVAILGYCFFKNQRKDFHGHHHHPPPTPASSTVSTTEDTEHLVYNHTTRPL hgf_human Hepatocyte growth factor HGF HPTA Scatter factor Hepatopoietin-A protein protein human Homo sapiens Human MWVTKLLPALLLQHVLLHLLLLPIAIPYAEGQRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS hgfac-kqlr-1 3200816 N/A 3200820 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3200821 unspecified role unspecified role unspecified role unspecified role Region 373-655 Region 373-655 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false hgfac-spint1-2 3200817 N/A 3200822 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster N/A 3200821 unspecified role unspecified role bait bait Region 373-655 Region 373-655 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false hgfac-kqlr-2 3200818 N/A 3200820 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3200821 unspecified role unspecified role unspecified role unspecified role Region 373-655 Region 373-655 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false hgf-hgfac-1 3200819 N/A 3200821 enzyme enzyme unspecified role unspecified role Region 373-655 Region 373-655 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3200823 enzyme target enzyme target substrate substrate unspecified role unspecified role 125I-label 125I-label 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster cleavage cleavage reaction cleavage false false false