DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ vives_bauza-2010-1 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Vives-Bauza C., Zhou C., Huang Y., Cui M., de Vries RL., Kim J., May J., Tocilescu MA., Liu W., Ko HS., Magrané J., Moore DJ., Dawson VL., Grailhe R., Dawson TM., Li C., Tieu K., Przedborski S. DIP original experiment: DIP-215833X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx PINK1-dependent recruitment of Parkin to mitochondria in mitophagy. Vives-Bauza C., Zhou C., Huang Y., Cui M., de Vries RL., Kim J., May J., Tocilescu MA., Liu W., Ko HS., Magrané J., Moore DJ., Dawson VL., Grailhe R., Dawson TM., Li C., Tieu K., Przedborski S. Only protein-protein interactions imex curation vives_bauza-2010-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Vives-Bauza C., Zhou C., Huang Y., Cui M., de Vries RL., Kim J., May J., Tocilescu MA., Liu W., Ko HS., Magrané J., Moore DJ., Dawson VL., Grailhe R., Dawson TM., Li C., Tieu K., Przedborski S. DIP original experiment: DIP-215835X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx PINK1-dependent recruitment of Parkin to mitochondria in mitophagy. Vives-Bauza C., Zhou C., Huang Y., Cui M., de Vries RL., Kim J., May J., Tocilescu MA., Liu W., Ko HS., Magrané J., Moore DJ., Dawson VL., Grailhe R., Dawson TM., Li C., Tieu K., Przedborski S. Only protein-protein interactions imex curation vives_bauza-2010-3 human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Vives-Bauza C., Zhou C., Huang Y., Cui M., de Vries RL., Kim J., May J., Tocilescu MA., Liu W., Ko HS., Magrané J., Moore DJ., Dawson VL., Grailhe R., Dawson TM., Li C., Tieu K., Przedborski S. DIP original experiment: DIP-215834X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx PINK1-dependent recruitment of Parkin to mitochondria in mitophagy. Vives-Bauza C., Zhou C., Huang Y., Cui M., de Vries RL., Kim J., May J., Tocilescu MA., Liu W., Ko HS., Magrané J., Moore DJ., Dawson VL., Grailhe R., Dawson TM., Li C., Tieu K., Przedborski S. Only protein-protein interactions imex curation prkn_human E3 ubiquitin-protein ligase parkin PRKN PARK2 Parkin RBR E3 ubiquitin-protein ligase Parkinson juvenile disease protein 2 protein protein human Homo sapiens Human MIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELRNDWTVQNCDLDQQSIVHIVQRPWRKGQEMNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFCRECKEAYHEGECSAVFEASGTTTQAYRVDERAAEQARWEAASKETIKKTTKPCPRCHVPVEKNGGCMHMKCPQPQCRLEWCWNCGCEWNRVCMGDHWFDV q9bxm7-1 Serine/threonine-protein kinase PINK1, mitochondrial PINK1 BRPK PTEN-induced putative kinase protein 1 protein protein human Homo sapiens Human MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAAGPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG park2-pink1-2 3200323 N/A 3200326 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3200327 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false pink1-ub-1 3200324 N/A 3200328 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human N/A 3200327 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false pink1-park2-2 3200325 N/A 3200326 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3200327 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human direct interaction direct interaction false false false