DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ natrajan-2009-1 in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134817X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134812X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134824X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-4 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134818X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-5 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134813X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-6 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 3 hybrid three hybrid 3-hybrid tri hybrid tri-hybrid assay 3 hybrid predetermined predetermined participant predetermined Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134822X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-7 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134825X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-8 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134814X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-9 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134819X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-10 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134823X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-11 in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134820X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-12 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134815X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-13 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134816X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation natrajan-2009-14 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. DIP original experiment: DIP-134821X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The structure of a DnaA/HobA complex from Helicobacter pylori provides insight into regulation of DNA replication in bacteria. Natrajan G., Noirot-Gros MF., Zawilak-Pawlik A., Kapp U., Terradot L. Only protein-protein interactions imex curation o25828_helpy HP_1230 protein protein helpy Helicobacter pylori Campylobacter pylori MKNFYDWIKEFVRDQGEFIAQQSGWLELERSSYAKLIAQTISHVLNGGSLLVSADSSRHWFLNYILSNLNPKDLKERPLLSVIDFNASSFYPKNDANLSLATIEMTYQNPMFWHVGKIENEGLKTILLSKIPSFLWLFEELKEDCLLLKEHDSLLDYKLLQLFKLFENALFSVLYNKVTL 91FE4AE113B32E15 dnaa_helpy Chromosomal replication initiator protein DnaA dnaA HP_1529 protein protein helpy Helicobacter pylori Campylobacter pylori MDTNNNIEKEILALVKQNPKVSLIEYENYFSQLKYNPNASKSDIAFFYAPNQVLCTTITAKYGALLKEILSQNKVGMHLAHSVDVRIEVAPKIQINAQSNINYKAIKTSVKDSYTFENFVVGSCNNTVYEIAKKVAQSDTPPYNPVLFYGGTGLGKTHILNAIGNHALEKHKKVVLVTSEDFLTDFLKHLDNKTMDSFKAKYRHCDFFLLDDAQFLQGKPKLEEEFFHTFNELHANSKQIVLISDRSPKNIAGLEDRLKSRFEWGITAKVMPPDLETKLSIVKQKCQLNQITLPEEVMEYIAQHISDNIRQMEGAIIKISVNANLMNASIDLNLAKTVLEDLQKDHAEGSSLENILLAVAQSLNLKSSEIKVSSRQKNVALARKLVVYFARLYTPNPTLSLAQFLDLKDHSSISKMYSGVKKMLEEEKSPFVLSLREEIKNRLNELNDKKTAFNSSE 975B30C9AB3A3C97 hoba-1 3199453 N/A 3199467 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false dnaa-hoba-1 3199454 N/A 3199468 unspecified role unspecified role unspecified role unspecified role Region 1-112 Region 1-112 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3199467 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hoba-hoba-1 3199455 N/A 3199467 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3199467 unspecified role unspecified role prey prey O25828:p.Ile41Thr I41T mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.His43Arg H43R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Val44Ala V44A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu51Phe L51F mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Asp55Gly D55G mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Phe61Cys F61C mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ser67Pro S67P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Arg77Cys R77C mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Val82Ala V82A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ile83Met I83M mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ser99Pro S99P mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Tyr107His Y107H mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Met111Val M111V mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Phe112Ser F112S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Val115Asp V115D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ile118Thr I118T mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Glu119Gly E119G mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu128Pro L128P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Phe138Leu F138L/S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu141Pro L141P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu146Ser L146S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu162Pro L162P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ala169Val A169V mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu170Pro L170P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Phe138Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false dnaa-hoba-2 3199456 N/A 3199468 unspecified role unspecified role bait bait Region 1-112 Region 1-112 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3199467 unspecified role unspecified role prey prey O25828:p.[Glu76Ala;Arg77Ala;Pro78Ala] E76A/R77A/P78A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu80Arg L80R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Tyr175Glu Y175E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dnaa-hoba-3 3199457 N/A 3199468 unspecified role unspecified role bait bait Region 1-112 Region 1-112 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3199467 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association false false false dnaa-dnaa-1 3199458 N/A 3199468 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3199468 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3199467 unspecified role unspecified role ancillary ancillary yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false dnaa-hoba-4 3199459 N/A 3199468 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3199467 unspecified role unspecified role prey prey Region 177-180 Region 177-180 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false dnaa-hoba-5 3199460 N/A 3199468 unspecified role unspecified role unspecified role unspecified role Region 1-112 Region 1-112 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3199467 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false dnaa-hoba-6 3199461 N/A 3199468 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3199467 unspecified role unspecified role prey prey O25828:p.Leu45Pro L45P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu174Pro L174P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ile41Thr I41T mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.His43Arg H43R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Val44Ala V44A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu51Phe L51F mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Asp55Gly D55G mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Phe61Cys F61C mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ser67Pro S67P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Arg77Cys R77C mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Val82Ala V82A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ile83Met I83M mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ser99Pro S99P mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Tyr107His Y107H mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Met111Val M111V mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Phe112Ser F112S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Val115Asp V115D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ile118Thr I118T mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Glu119Gly E119G mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu128Pro L128P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Phe138Leu F138L/S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu141Pro L141P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu146Ser L146S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu162Pro L162P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ala169Val A169V mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu170Pro L170P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Phe138Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false hoba-dnaa-1 3199462 N/A 3199468 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3199467 unspecified role unspecified role bait bait O25828:p.Leu80Arg L80R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.His43Arg H43R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu51Phe L51F/S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ser67Pro S67P mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu69Pro L69P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ser88Pro S88P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu127Pro L127P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu137Pro L137P mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Phe138Ser F138S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu146Ser L146S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Phe166Ser F166S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu174Pro L174P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.[Glu76Ala;Arg77Ala;Pro78Ala] E76A/R77A/P78A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O25828:p.Tyr175Glu Y175E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu51Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false dnaa-hoba-7 3199463 N/A 3199468 unspecified role unspecified role unspecified role unspecified role Region 1-112 Region 1-112 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3199467 unspecified role unspecified role unspecified role unspecified role O25828:p.[Glu76Ala;Arg77Ala;Pro78Ala] E76A/R77A/P78A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu80Arg L80R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Tyr175Glu Y175E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false hoba-2 3199464 N/A 3199467 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false dnaa-hoba-8 3199465 N/A 3199468 unspecified role unspecified role unspecified role unspecified role Region 1-112 Region 1-112 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3199467 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hoba-hoba-2 3199466 N/A 3199467 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3199467 unspecified role unspecified role bait bait O25828:p.Leu80Arg L80R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.His43Arg H43R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu51Phe L51F/S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ser67Pro S67P mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu69Pro L69P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Ser88Pro S88P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu127Pro L127P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu137Pro L137P mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Phe138Ser F138S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu146Ser L146S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Phe166Ser F166S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O25828:p.Leu51Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false