IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ reed-2010-4 mouse-kidney Mus musculus kidney kidney [Renal] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Reed AA., Loh NY., Terryn S., Lippiat JD., Partridge C., Galvanovskis J., Williams SE., Jouret F., Wu FT., Courtoy PJ., Nesbit MA., Rorsman P., Devuyst O., Ashcroft FM., Thakker RV. rajesh.thakker@ndm.ox.ac.uk Results from figure 1G have not been entered as the species origin of Myc CLC-4 and Myc-CLC-7is not stated, and the authors did not reply to a request for information. American journal of physiology. Renal physiology 2010 Only protein-protein interactions imex curation imex curation reed-2010-5 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Reed AA., Loh NY., Terryn S., Lippiat JD., Partridge C., Galvanovskis J., Williams SE., Jouret F., Wu FT., Courtoy PJ., Nesbit MA., Rorsman P., Devuyst O., Ashcroft FM., Thakker RV. rajesh.thakker@ndm.ox.ac.uk Results from figure 1G have not been entered as the species origin of Myc CLC-4 and Myc-CLC-7is not stated, and the authors did not reply to a request for information. American journal of physiology. Renal physiology 2010 Only protein-protein interactions imex curation imex curation reed-2010-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Reed AA., Loh NY., Terryn S., Lippiat JD., Partridge C., Galvanovskis J., Williams SE., Jouret F., Wu FT., Courtoy PJ., Nesbit MA., Rorsman P., Devuyst O., Ashcroft FM., Thakker RV. rajesh.thakker@ndm.ox.ac.uk Results from figure 1G have not been entered as the species origin of Myc CLC-4 and Myc-CLC-7is not stated, and the authors did not reply to a request for information. adult human kidney cDNA library American journal of physiology. Renal physiology 2010 Only protein-protein interactions imex curation imex curation reed-2010-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Reed AA., Loh NY., Terryn S., Lippiat JD., Partridge C., Galvanovskis J., Williams SE., Jouret F., Wu FT., Courtoy PJ., Nesbit MA., Rorsman P., Devuyst O., Ashcroft FM., Thakker RV. rajesh.thakker@ndm.ox.ac.uk Results from figure 1G have not been entered as the species origin of Myc CLC-4 and Myc-CLC-7is not stated, and the authors did not reply to a request for information. American journal of physiology. Renal physiology 2010 Only protein-protein interactions imex curation imex curation reed-2010-3 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Reed AA., Loh NY., Terryn S., Lippiat JD., Partridge C., Galvanovskis J., Williams SE., Jouret F., Wu FT., Courtoy PJ., Nesbit MA., Rorsman P., Devuyst O., Ashcroft FM., Thakker RV. rajesh.thakker@ndm.ox.ac.uk Results from figure 1G have not been entered as the species origin of Myc CLC-4 and Myc-CLC-7is not stated, and the authors did not reply to a request for information. American journal of physiology. Renal physiology 2010 Only protein-protein interactions imex curation imex curation reed-2010-6 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Reed AA., Loh NY., Terryn S., Lippiat JD., Partridge C., Galvanovskis J., Williams SE., Jouret F., Wu FT., Courtoy PJ., Nesbit MA., Rorsman P., Devuyst O., Ashcroft FM., Thakker RV. rajesh.thakker@ndm.ox.ac.uk Results from figure 1G have not been entered as the species origin of Myc CLC-4 and Myc-CLC-7is not stated, and the authors did not reply to a request for information. American journal of physiology. Renal physiology 2010 Only protein-protein interactions imex curation imex curation clcn5_mouse H(+)/Cl(-) exchange transporter 5 Clcn5 Clc5 Chloride transporter ClC-5 Chloride channel protein 5 protein protein mouse Mus musculus Mouse MAMWQGAMDNRGFHQGSFSSFQSSSSDEDLMDIPGTAMDFSMRDDVPPLDREIEGNKSYNGGGIGSSNRVMDFLEEPIPGVGTYDDFNTIDWVREKSRDRDRHREITNKSKESTWALIHSVSDAFSGWLLMLLIGLLSGSLAGLIDISAHWMTDLKEGICTGGFWFNHEHCCWNSEHVTFEHRDKCPEWNSWAQLIINTDQGAFAYIVNYFMYVLWALLFAFLAVSLVKAFAPYACGSGIPEIKTILSGFIIRGYLGKWTLVIKTITLVLAVSSGLSLGKEGPLVHVACCCGNILCHCFNKYRKNEAKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEFHTPWHLFELVPFIVLGIFGGLWGALFIRTNIAWCRKRKTTQLGKYPVVEVLIVTAITAILAFPNEYTRMSTSELISELFNDCGLLDSSKLCDYENHFNTSKGGELPDRPAGVGIYSAMWQLALTLILKIVITIFTFGMKIPSGLFIPSMAVGAIAGRLLGVGMEQLAYYHHDWGIFNSWCSQGADCITPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAMTSKWVADALGREGIYDAHIRLNGYPFLEAKEEFAHKTLAMDVMKPRRNDPLLTVLTQDSMTVEDVETIISETTYSGFPVVVSRESQRLVGFVLRRDLIISIENARKKQDGVVSTSIIYFTEHSPPMPPYTPPTLKLRNILDLSPFTVTDLTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDVLKHIAQMANQDPDSILFN mint Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May play an important role in renal tubular function kif3b_mouse Kinesin-like protein KIF3B Kif3b Microtubule plus end-directed kinesin motor 3B protein protein mouse Mus musculus Mouse MSKLKSSESVRVVVRCRPMNGKEKAASYDKVVDVDVKLGQVSVKNPKGTSHEMPKTFTFDAVYDWNAKQFELYDETFRPLVDSVLQGFNGTIFAYGQTGTGKTYTMEGVRGDPEKRGVIPNSFDHIFTHISRSQNQQYLVRASYLEIYQEEIRDLLSKDQTKRLELKERPDTGVYVKDLSSFVTKSVKEIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVITIECSEVGLDGENHIRVGKLNLVDLAGSERQAKTGAQGERLKEATKINLSLSALGNVISALVDGKSTHIPYRDSKLTRLLQDSLGGNAKTVMVANVGPASYNVEETLTTLRYANRAKNIKNKPRVNEDPKDALLREFQEEIARLKAQLEKRSIGRRKRREKRREGGGSGGGGEEEEEEGEEGEEDGDDKDDYWREQQEKLEIEKRAIVEDHSLVAEEKMRLLKEKEKKMEDLRREKDAAEMLGAKIKAMESKLLVGGKNIVDHTNEQQKILEQKRQEIAEQKRREREIQQQMESRDEETLELKETYTSLQQEVDIKTKKLKKLFSKLQAVKAEIHDLQEEHIKERQELEQTQNELTRELKLKHLIIENFIPLEEKNKIMNRSFFDDEEDHWKLHPITRLENQQMMKRPVSAVGYKRPLSQHARMSMMIRPEPRYRAENIMLLELDMPSRTTRDYEGPAISPKVQAALDAALQDEDEIQVDASSFESTASRKPKARPKSGRKSGSSSSSSGNPASQFYPQSRGLVPK kif3a_mouse Kinesin-like protein KIF3A Kif3a Kif3 Microtubule plus end-directed kinesin motor 3A protein protein mouse Mus musculus Mouse MPINKSEKPESCDNVKVVVRCRPLNEREKSMCYRQAVSVDEMRGTITVHKTDSSNEPPKTFTFDTVFGPESKQLDVYNLTARPIIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAVPGLRGVIPNSFAHIFGHIAKAEGDTRFLVRVSYLEIYNEEVRDLLGKDQTQRLEVKERPDVGVYIKDLSAYVVNNADDMDRIMTLGHKNRSVGATNMNEHSSRSHAIFTITIECSEKGVDGNMHVRMGKLHLVDLAGSERQAKTGATGQRLKEATKINLSLSTLGNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKARINEDPKDALLRQFQKEIEELKKKLEEGEEVSGSDISGSEEDDEEGELGEDGEKKKKRRDQAGKKKVSPDKMVEMQAKIDEERKALETKLDMEEEERNKARAELERREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRRRAEQLRKELEEKEQERLDIEEKYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPQDYQEMIENYVHWNEDIGEWQLKCVAYTGNNMRKQTPVPDKKERDPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKGKARPKMGRRKRSAKPETVIDSLLQ kifa3_mouse Kinesin-associated protein 3 Kifap3 protein protein mouse Mus musculus Mouse MQGEDARYLKRKVKGGNIDVHPSEKALIVQYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSLARKVVEECKLIHPSKLSEVEQLLYYLQNRRDSLPGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSHFHGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDLENQTLRKDYDKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNENYKQIAMCVLYHISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKLKDPLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISSDEEEEFVIECLGTLANLTIPDLDWELVLKEYKLVPFLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVESRQLDESEQYLYGDDRIEPYIHEGDILERPDLFYNSDGLITSEGAISPDFFNDFHLQNGDVVGQHAFPGSLGMDGFGQPLGILGRPATAYGFRPDEPYYYSFGSR kif3b_human Kinesin-like protein KIF3B KIF3B KIAA0359 HH0048 Microtubule plus end-directed kinesin motor 3B protein protein human Homo sapiens Human MSKLKSSESVRVVVRCRPMNGKEKAASYDKVVDVDVKLGQVSVKNPKGTAHEMPKTFTFDAVYDWNAKQFELYDETFRPLVDSVLQGFNGTIFAYGQTGTGKTYTMEGIRGDPEKRGVIPNSFDHIFTHISRSQNQQYLVRASYLEIYQEEIRDLLSKDQTKRLELKERPDTGVYVKDLSSFVTKSVKEIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVITIECSEVGLDGENHIRVGKLNLVDLAGSERQAKTGAQGERLKEATKINLSLSALGNVISALVDGKSTHIPYRDSKLTRLLQDSLGGNAKTVMVANVGPASYNVEETLTTLRYANRAKNIKNKPRVNEDPKDALLREFQEEIARLKAQLEKRSIGRRKRREKRREGGGSGGGGEEEEEEGEEGEEEGDDKDDYWREQQEKLEIEKRAIVEDHSLVAEEKMRLLKEKEKKMEDLRREKDAAEMLGAKIKAMESKLLVGGKNIVDHTNEQQKILEQKRQEIAEQKRREREIQQQMESRDEETLELKETYSSLQQEVDIKTKKLKKLFSKLQAVKAEIHDLQEEHIKERQELEQTQNELTRELKLKHLIIENFIPLEEKSKIMNRAFFDEEEDHWKLHPITRLENQQMMKRPVSAVGYKRPLSQHARMSMMIRPEARYRAENIVLLELDMPSRTTRDYEGPAIAPKVQAALDAALQDEDEIQVDASSFESTANKKSKARPKSGRKSGSSSSSSGTPASQLYPQSRGLVPK clcn5_human H(+)/Cl(-) exchange transporter 5 CLCN5 CLCK2 Chloride channel protein 5 Chloride transporter ClC-5 protein protein human Homo sapiens Human MAMWQGAMDNRGFQQGSFSSFQNSSSDEDLMDIPATAMDFSMRDDVPPLDREVGEDKSYNGGGIGSSNRIMDFLEEPIPGVGTYDDFNTIDWVREKSRDRDRHREITNKSKESTWALIHSVSDAFSGWLLMLLIGLLSGSLAGLIDISAHWMTDLKEGICTGGFWFNHEHCCWNSEHVTFEERDKCPEWNSWSQLIISTDEGAFAYIVNYFMYVLWALLFAFLAVSLVKVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLVIKTITLVLAVSSGLSLGKEGPLVHVACCCGNILCHCFNKYRKNEAKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEFHTPWHLFELVPFILLGIFGGLWGALFIRTNIAWCRKRKTTQLGKYPVIEVLVVTAITAILAFPNEYTRMSTSELISELFNDCGLLDSSKLCDYENRFNTSKGGELPDRPAGVGVYSAMWQLALTLILKIVITIFTFGMKIPSGLFIPSMAVGAIAGRLLGVGMEQLAYYHQEWTVFNSWCSQGADCITPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAMTSKWVADALGREGIYDAHIRLNGYPFLEAKEEFAHKTLAMDVMKPRRNDPLLTVLTQDSMTVEDVETIISETTYSGFPVVVSRESQRLVGFVLRRDLIISIENARKKQDGVVSTSIIYFTEHSPPLPPYTPPTLKLRNILDLSPFTVTDLTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDVLKHIAQMANQDPDSILFN q345tq354q-1 2876470 N/A 2876476 unspecified role unspecified role bait bait N/A 2876477 unspecified role unspecified role prey prey physical association physical association false false false 1D-1 kifap3-kif3a-1 2876470 N/A 2876478 unspecified role unspecified role prey prey N/A 2876479 unspecified role unspecified role bait bait physical association physical association false false false 1D-2 kifap3-kif3a-3 2876470 N/A 2876478 unspecified role unspecified role prey prey N/A 2876477 unspecified role unspecified role bait bait N/A 2876479 unspecified role unspecified role prey prey association association false false false 1D-3 clcn5-kif3b-4 2876471 N/A 2876480 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cc_domain sufficient to bind sufficient binding region sufficient to bind range range range range false globular_domain sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 2876481 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1 E/F clcn5-kif3b-5 2876471 N/A 2876480 unspecified role unspecified role bait bait cc_domain sufficient to bind sufficient binding region sufficient to bind range range range range false globular_domain sufficient to bind sufficient binding region sufficient to bind range range range range false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2876481 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1 E/Fi clcn5-kif3b-1 2876472 N/A 2876480 unspecified role unspecified role prey prey c-terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2876481 unspecified role unspecified role bait bait c_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false no figure clcn5-kif3b-2 2876473 N/A 2876480 unspecified role unspecified role prey prey N/A 2876481 unspecified role unspecified role bait bait c_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B clcn5-kif3b-3 2876474 N/A 2876480 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false coiled_coil+globular_domains sufficient to bind sufficient binding region sufficient to bind range range range range false ecoli Escherichia coli (strain K12) N/A 2876481 unspecified role unspecified role prey prey cell lysate cell lysate in vitro translated in vitro translated protein in vitro translated c-terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association false false false 1C Two bands were detected for CLC-5. clcn5-kif3b-6 2876475 N/A 2876481 unspecified role unspecified role neutral component neutral component n_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2876480 unspecified role unspecified role neutral component neutral component n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 2A CLC-5 was concentrated at the plasma membrane, and in punctate structures throughout the cytoplasm, where it showed partial colocalization with KIF3B.