DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ melcher-2009-1 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219627X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-2 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219616X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219639X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-4 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219617X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-5 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219637X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-6 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219618X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-7 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219638X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-8 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219628X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-9 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219630X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-10 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219619X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-11 in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219641X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-12 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219629X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-13 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219631X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-14 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219620X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-15 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219640X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-16 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219621X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-17 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219622X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-18 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219632X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-19 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219642X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-20 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219634X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-21 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219623X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-22 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219633X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-23 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219624X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-24 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219635X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-25 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219625X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-26 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219636X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation melcher-2009-27 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. DIP original experiment: DIP-219626X Nature 2009 IMEx A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K., Ng LM., Zhou XE., Soon FF., Xu Y., Suino-Powell KM., Park SY., Weiner JJ., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson FC., Jensen DR., Yong EL., Volkman BF., Cutler SR., Zhu JK., Xu HE. Only protein-protein interactions imex curation o04719-1 Protein phosphatase 2C 77 PP2C77 ABI2 Protein ABSCISIC ACID-INSENSITIVE 2 Protein phosphatase 2C ABI2 MHM17.19 At5g57050 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDEVSPAVAVPFRPFTDPHAGLRGYCNGESRVTLPESSCSGDGAMKDSSFEINTRQDSLTSSSSAMAGVDISAGDEINGSDEFDPRSMNQSEKKVLSRTESRSLFEFKCVPLYGVTSICGRRPEMEDSVSTIPRFLQVSSSSLLDGRVTNGFNPHLSAHFFGVYDGHGGSQVANYCRERMHLALTEEIVKEKPEFCDGDTWQEKWKKALFNSFMRVDSEIETVAHAPETVGSTSVVAVVFPTHIFVANCGDSRAVLCRGKTPLALSVDHKPDRDDEAARIEAAGGKVIRWNGARVFGVLAMSRSIGDRYLKPSVIPDPEVTSVRRVKEDDCLILASDGLWDVMTNEEVCDLARKRILLWHKKNAMAGEALLPAEKRGEGKDPAAMSAAEYLSKMALQKGSKDNISVVVVDLKGIRKFKSKSLN pyl3_arath Abscisic acid receptor PYL3 PYL3 RCAR13 PYR1-like protein 3 Regulatory components of ABA receptor 13 F3N23.20 At1g73000 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNLAPIHDPSSSSTTTTSSSTPYGLTKDEFSTLDSIIRTHHTFPRSPNTCTSLIAHRVDAPAHAIWRFVRDFANPNKYKHFIKSCTIRVNGNGIKEIKVGTIREVSVVSGLPASTSVEILEVLDEEKRILSFRVLGGEHRLNNYRSVTSVNEFVVLEKDKKKRVYSVVLESYIVDIPQGNTEEDTRMFVDTVVKSNLQNLAVISTASPT p2c16_arath Protein phosphatase 2C 16 HAB1 P2C-HA Protein phosphatase 2C HAB1 AtP2C-HA Protein HYPERSENSITIVE TO ABA 1 F28P22.4 At1g72770 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEMTPAVAMTLSLAANTMCESSPVEITQLKNVTDAADLLSDSENQSFCNGGTECTMEDVSELEEVGEQDLLKTLSDTRSGSSNVFDEDDVLSVVEDNSAVISEGLLVVDAGSELSLSNTAMEIDNGRVLATAIIVGESSIEQVPTAEVLIAGVNQDTNTSEVVIRLPDENSNHLVKGRSVYELDCIPLWGTVSIQGNRSEMEDAFAVSPHFLKLPIKMLMGDHEGMSPSLTHLTGHFFGVYDGHGGHKVADYCRDRLHFALAEEIERIKDELCKRNTGEGRQVQWDKVFTSCFLTVDGEIEGKIGRAVVGSSDKVLEAVASETVGSTAVVALVCSSHIVVSNCGDSRAVLFRGKEAMPLSVDHKPDREDEYARIENAGGKVIQWQGARVFGVLAMSRSIGDRYLKPYVIPEPEVTFMPRSREDECLILASDGLWDVMNNQEVCEIARRRILMWHKKNGAPPLAERGKGIDPACQAAADYLSMLALQKGSKDNISIIVIDLKAQRKFKTRT pyl1_arath Abscisic acid receptor PYL1 At5g46790 PYL1 RCAR12 PYR1-like protein 1 ABI1-binding protein 6 Regulatory components of ABA receptor 9 MZA15.21 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANSESSSSPVNEEENSQRISTLHHQTMPSDLTQDEFTQLSQSIAEFHTYQLGNGRCSSLLAQRIHAPPETVWSVVRRFDRPQIYKHFIKSCNVSEDFEMRVGCTRDVNVISGLPANTSRERLDLLDDDRRVTGFSITGGEHRLRNYKSVTTVHRFEKEEEEERIWTVVLESYVVDVPEGNSEEDTRLFADTVIRLNLQKLASITEAMNRNNNNNNSSQVR pyl2_arath Abscisic acid receptor PYL2 PYL2 RCAR14 PYR1-like protein 2 Regulatory components of ABA receptor 14 T19L18.15 At2g26040 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSSPAVKGLTDEEQKTLEPVIKTYHQFEPDPTTCTSLITQRIHAPASVVWPLIRRFDNPERYKHFVKRCRLISGDGDVGSVREVTVISGLPASTSTERLEFVDDDHRVLSFRVVGGEHRLKNYKSVTSVNEFLNQDSGKVYTVVLESYTVDIPEGNTEEDTKMFVDTVVKLNLQKLGVAATSAPMHDDE p2c56_arath Protein phosphatase 2C 56 Protein ABSCISIC ACID-INSENSITIVE 1 ABI1 At4g26080 F20B18.190 Protein phosphatase 2C ABI1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEVSPAIAGPFRPFSETQMDFTGIRLGKGYCNNQYSNQDSENGDLMVSLPETSSCSVSGSHGSESRKVLISRINSPNLNMKESAAADIVVVDISAGDEINGSDITSEKKMISRTESRSLFEFKSVPLYGFTSICGRRPEMEDAVSTIPRFLQSSSGSMLDGRFDPQSAAHFFGVYDGHGGSQVANYCRERMHLALAEEIAKEKPMLCDGDTWLEKWKKALFNSFLRVDSEIESVAPETVGSTSVVAVVFPSHIFVANCGDSRAVLCRGKTALPLSVDHKPDREDEAARIEAAGGKVIQWNGARVFGVLAMSRSIGDRYLKPSIIPDPEVTAVKRVKEDDCLILASDGVWDVMTDEEACEMARKRILLWHKKNAVAGDASLLADERRKEGKDPAAMSAAEYLSKLAIQRGSKDNISVVVVDLKPRRKLKSKPLN pyl4_arath Abscisic acid receptor PYL4 At2g38310 PYL4 ABIP2 RCAR10 PYR1-like protein 4 ABI1-binding protein 2 Regulatory components of ABA receptor 10 T19C21.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLAVHRPSSAVSDGDSVQIPMMIASFQKRFPSLSRDSTAARFHTHEVGPNQCCSAVIQEISAPISTVWSVVRRFDNPQAYKHFLKSCSVIGGDGDNVGSLRQVHVVSGLPAASSTERLDILDDERHVISFSVVGGDHRLSNYRSVTTLHPSPISGTVVVESYVVDVPPGNTKEETCDFVDVIVRCNLQSLAKIAENTAAESKKKMSL srk2e_arath Serine/threonine-protein kinase SRK2E SNRK2.6 Serine/threonine-protein kinase OST1 Protein OPEN STOMATA 1 SNF1-related kinase 2.6 F17I5.140 SRK2E OST1 At4g33950 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDRPAVSGPMDLPIMHDSDRYELVKDIGSGNFGVARLMRDKQSNELVAVKYIERGEKIDENVKREIINHRSLRHPNIVRFKEVILTPTHLAIVMEYASGGELFERICNAGRFSEDEARFFFQQLISGVSYCHAMQVCHRDLKLENTLLDGSPAPRLKICDFGYSKSSVLHSQPKSTVGTPAYIAPEVLLKKEYDGKVADVWSCGVTLYVMLVGAYPFEDPEEPKNFRKTIHRILNVQYAIPDYVHISPECRHLISRIFVADPAKRISIPEIRNHEWFLKNLPADLMNDNTMTTQFDESDQPGQSIEEIMQIIAEATVPPAGTQNLNHYLTGSLDIDDDMEEDLESDLDDLDIDSSGEIVYAM pyl5_arath Abscisic acid receptor PYL5 At5g05440 PYL5 ABIP3 RCAR8 PYR1-like protein 5 ABI1-binding protein 3 Regulatory components of ABA receptor 8 K18I23.25 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRSPVQLQHGSDATNGFHTLQPHDQTDGPIKRVCLTRGMHVPEHVAMHHTHDVGPDQCCSSVVQMIHAPPESVWALVRRFDNPKVYKNFIRQCRIVQGDGLHVGDLREVMVVSGLPAVSSTERLEILDEERHVISFSVVGGDHRLKNYRSVTTLHASDDEGTVVVESYIVDVPPGNTEEETLSFVDTIVRCNLQSLARSTNRQ pyr1_arath Abscisic acid receptor PYR1 PYR1 ABIP6 At4g17870 RCAR11 Protein PYRABACTIN RESISTANCE 1 ABI1-binding protein 6 Regulatory components of ABA receptor 11 T6K21.50 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPSELTPEERSELKNSIAEFHTYQLDPGSCSSLHAQRIHAPPELVWSIVRRFDKPQTYKHFIKSCSVEQNFEMRVGCTRDVIVISGLPANTSTERLDILDDERRVTGFSIIGGEHRLTNYKSVTTVHRFEKENRIWTVVLESYVVDMPEGNSEDDTRMFADTVVKLNLQKLATVAEAMARNSGDGSGSQVT pyl6_arath Abscisic acid receptor PYL6 T3G21 At2g40330 PYL6 ABIP5 RCAR9 PYR1-like protein 6 ABI1-binding protein 5 Regulatory components of ABA receptor 9 T7M7.15 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPTSIQFQRSSTAAEAANATVRNYPHHHQKQVQKVSLTRGMADVPEHVELSHTHVVGPSQCFSVVVQDVEAPVSTVWSILSRFEHPQAYKHFVKSCHVVIGDGREVGSVREVRVVSGLPAAFSLERLEIMDDDRHVISFSVVGGDHRLMNYKSVTTVHESEEDSDGKKRTRVVESYVVDVPAGNDKEETCSFADTIVRCNLQSLAKLAENTSKFS abi2-pyl3-1 3198582 N/A 3198609 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198610 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hab1-pyl1-1 3198583 N/A 3198611 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198612 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hab1-pyl2-1 3198584 N/A 3198613 unspecified role unspecified role unspecified role unspecified role Region 14-188 Region 14-188 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198611 unspecified role unspecified role unspecified role unspecified role Region 172-511 Region 172-511 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false abi1-pyl1-7 3198585 N/A 3198614 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198612 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pyl1-2 3198586 N/A 3198612 unspecified role unspecified role unspecified role unspecified role Region 36-211 Region 36-211 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false abi2-pyl1-1 3198587 N/A 3198609 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198612 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pyl1-4 3198588 N/A 3198612 unspecified role unspecified role unspecified role unspecified role Region 36-211 Region 36-211 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false hab1-pyl4-1 3198589 N/A 3198615 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198611 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false abi2-pyl4-1 3198590 N/A 3198609 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198615 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hab1-pyl2-2 3198591 N/A 3198613 unspecified role unspecified role unspecified role unspecified role O80992:p.His119Ala H119A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O80992:p.Arg120Ala R120A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198611 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hab1-srk2e-1 3198592 N/A 3198616 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 170-180 Region 170-180 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false pS175 pS175 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3198611 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation false false false abi1-pyl4-1 3198593 N/A 3198614 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198615 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hab1-pyl5-5 3198594 N/A 3198617 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198611 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false abi1-pyl2-2 3198595 N/A 3198614 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198613 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hab1-pyl2-3 3198596 N/A 3198613 unspecified role unspecified role unspecified role unspecified role Region 14-188 Region 14-188 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198611 unspecified role unspecified role unspecified role unspecified role Region 172-511 Region 172-511 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false abi2-pyl2-1 3198597 N/A 3198609 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198613 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hab1-pyr1-3 3198598 N/A 3198618 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198611 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false abi1-pyl5-1 3198599 N/A 3198614 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198617 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pyr1-hab1-5 3198600 N/A 3198618 unspecified role unspecified role bait bait O49686:p.His115Ala H115A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3198611 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false hab1-pyl6-3 3198601 N/A 3198619 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198611 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false abi1-pyr1-1 3198602 N/A 3198614 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198618 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false abi2-pyl5-1 3198603 N/A 3198609 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198617 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false abi2-pyr1-1 3198604 N/A 3198609 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198618 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false abi1-pyl6-1 3198605 N/A 3198614 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198619 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hab1-pyl3-1 3198606 N/A 3198610 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198611 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false abi2-pyl6-1 3198607 N/A 3198609 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198619 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false abi1-pyl3-1 3198608 N/A 3198614 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3198610 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false