DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yin-2009-15 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N. DIP original experiment: DIP-133331X Nature structural & molecular biology 2009 IMEx Structural insights into the mechanism of abscisic acid signaling by PYL proteins. Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N. Only protein-protein interactions imex curation yin-2009-16 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N. DIP original experiment: DIP-133333X Nature structural & molecular biology 2009 IMEx Structural insights into the mechanism of abscisic acid signaling by PYL proteins. Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N. Only protein-protein interactions imex curation yin-2009-17 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N. DIP original experiment: DIP-133328X Nature structural & molecular biology 2009 IMEx Structural insights into the mechanism of abscisic acid signaling by PYL proteins. Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N. Only protein-protein interactions imex curation yin-2009-18 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N. DIP original experiment: DIP-133329X Nature structural & molecular biology 2009 IMEx Structural insights into the mechanism of abscisic acid signaling by PYL proteins. Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N. Only protein-protein interactions imex curation yin-2009-19 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N. DIP original experiment: DIP-133330X Nature structural & molecular biology 2009 IMEx Structural insights into the mechanism of abscisic acid signaling by PYL proteins. Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N. Only protein-protein interactions imex curation yin-2009-20 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N. DIP original experiment: DIP-133332X Nature structural & molecular biology 2009 IMEx Structural insights into the mechanism of abscisic acid signaling by PYL proteins. Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N. Only protein-protein interactions imex curation pyl1_arath Abscisic acid receptor PYL1 At5g46790 PYL1 RCAR12 PYR1-like protein 1 ABI1-binding protein 6 Regulatory components of ABA receptor 9 MZA15.21 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANSESSSSPVNEEENSQRISTLHHQTMPSDLTQDEFTQLSQSIAEFHTYQLGNGRCSSLLAQRIHAPPETVWSVVRRFDRPQIYKHFIKSCNVSEDFEMRVGCTRDVNVISGLPANTSRERLDLLDDDRRVTGFSITGGEHRLRNYKSVTTVHRFEKEEEEERIWTVVLESYVVDVPEGNSEEDTRLFADTVIRLNLQKLASITEAMNRNNNNNNSSQVR p2c56_arath Protein phosphatase 2C 56 Protein ABSCISIC ACID-INSENSITIVE 1 ABI1 At4g26080 F20B18.190 Protein phosphatase 2C ABI1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEVSPAIAGPFRPFSETQMDFTGIRLGKGYCNNQYSNQDSENGDLMVSLPETSSCSVSGSHGSESRKVLISRINSPNLNMKESAAADIVVVDISAGDEINGSDITSEKKMISRTESRSLFEFKSVPLYGFTSICGRRPEMEDAVSTIPRFLQSSSGSMLDGRFDPQSAAHFFGVYDGHGGSQVANYCRERMHLALAEEIAKEKPMLCDGDTWLEKWKKALFNSFLRVDSEIESVAPETVGSTSVVAVVFPSHIFVANCGDSRAVLCRGKTALPLSVDHKPDREDEAARIEAAGGKVIQWNGARVFGVLAMSRSIGDRYLKPSIIPDPEVTAVKRVKEDDCLILASDGVWDVMTDEEACEMARKRILLWHKKNAVAGDASLLADERRKEGKDPAAMSAAEYLSKLAIQRGSKDNISVVVVDLKPRRKLKSKPLN pyl2_arath Abscisic acid receptor PYL2 PYL2 RCAR14 PYR1-like protein 2 Regulatory components of ABA receptor 14 T19L18.15 At2g26040 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSSPAVKGLTDEEQKTLEPVIKTYHQFEPDPTTCTSLITQRIHAPASVVWPLIRRFDNPERYKHFVKRCRLISGDGDVGSVREVTVISGLPASTSTERLEFVDDDHRVLSFRVVGGEHRLKNYKSVTSVNEFLNQDSGKVYTVVLESYTVDIPEGNTEEDTKMFVDTVVKLNLQKLGVAATSAPMHDDE abi1-pyl1-4 3198493 N/A 3198499 unspecified role unspecified role prey prey Region 19-210 Region 19-210 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198500 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Core-domain Region 118-425 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false abi1-pyl1-5 3198494 N/A 3198499 unspecified role unspecified role unspecified role unspecified role Region 19-210 Region 19-210 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198500 unspecified role unspecified role unspecified role unspecified role Core-domain Region 118-425 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pyl2-1 3198495 N/A 3198501 unspecified role unspecified role unspecified role unspecified role O80992:p.[Phe66Ala;Ile88Ala;Asn157Ala;Met164Ala;Phe165Ala] F66A/I88A/N157A/M164A/F165A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pyl2-2 3198496 N/A 3198501 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false abi1-pyl2-1 3198497 N/A 3198500 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3198501 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false abi1-pyl1-6 3198498 N/A 3198499 unspecified role unspecified role unspecified role unspecified role Region 19-210 Region 19-210 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198500 unspecified role unspecified role unspecified role unspecified role Core-domain Region 118-425 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false